A5082636959- Food Allergy and Anaphylaxis Research
- Allergic Rhinitis and Sensitization
- Dermatology and Skin Diseases
- Asthma and respiratory diseases
- Immunotherapy and Immune Responses
- Veterinary Oncology Research
- Virus-based gene therapy research
- T-cell and B-cell Immunology
- Immune Response and Inflammation
- Mast cells and histamine
- Animal Genetics and Reproduction
- Antimicrobial Peptides and Activities
- Contact Dermatitis and Allergies
- Herpesvirus Infections and Treatments
- Immune Cell Function and Interaction
- Peptidase Inhibition and Analysis
- Cancer Research and Treatments
- RNA Interference and Gene Delivery
- HIV Research and Treatment
- Probiotics and Fermented Foods
- Glycosylation and Glycoproteins Research
- Cancer-related Molecular Pathways
- NF-κB Signaling Pathways
- SARS-CoV-2 and COVID-19 Research
- Chemokine receptors and signaling
RIKEN Center for Sustainable Resource Science
2024
Allergy Therapeutics (United Kingdom)
2008-2021
RIKEN Center for Integrative Medical Sciences
2004-2021
Kansai University
2017
The University of Tokyo
1999-2015
RIKEN
2004-2012
Iwate Medical University
2012
Yamaguchi University
2001-2012
North Central State College
2007
Teijin (Japan)
1993-2007
A novel trypsin-like protease was purified to homogeneity from the sputum of patients with chronic airway diseases, by sequential chromatographic procedures. The enzyme migrated on SDS-polyacrylamide gel electrophoresis a position corresponding molecular weight 28 kDa under both reducing and non-reducing conditions, showed an apparent 27 filtration, indicating that it exists as monomer. It had NH2-terminal sequence Ile-Leu-Gly-Gly-Thr-Glu-Ala-Glu-Glu-Gly-Ser-Trp-Pro-Trp-Gln-Val-Ser-Leu-...
Previously we isolated a trypsin-like enzyme designated human airway protease from the sputum of patients with chronic diseases. This paper describes cDNA cloning, characterization primary protein structure deduced cDNA, and gene expression this in various tissues. We obtained an entire 1517-base pair sequence open reading frame encoding polypeptide 418-amino acid residues. The consisted 232-residue catalytic region 186-residue noncatalytic hydrophobic putative transmembrane domain near NH2...
We investigated the hematologic abnormalities and prognoses in 16 cats with myelodysplastic syndromes (MDS). Nonregenerative anemia, thrombocytopenia, neutropenia were observed 15, 13, 4, respectively, of MDS. Morphologic characteristic MDS included megaloblastoid rubricytes (9 cats), hyposegmentation neutrophils (7 nuclear abnormality (10 cats) (13 micromegakaryocytes cats). Disease these was subclassified into refractory anemia (RA; 8 RA excess blasts (RAEB; 5 RAEB transformation (RAEB T;...
To determine the effects of manual acupuncture stimulation (MAS) on skin sympathetic nerve activity (SSNA), SSNA and blood flow (SBF) were measured during a resting period MAS. Twelve healthy male subjects divided into an group (n = 7) control 5). was recorded from left median at elbow using microneurography, while SBF laser Doppler flowmeter. In group, MAS delivered to LI 4 point in right thenar muscle. The needle retained for 2 minutes before being removed. recordings performed total 12...
Japanese cedar (Cryptomeria japonica) pollinosis is the most prevalent allergy in Japan. Recently, pollen allergen Cry j 3 was cloned as a homologue of Jun 3, which major from mountain (Juniperus ashei) pollen. However, native has not been isolated and there are no reports on its allergenic activity. The aims this study were to isolate assess immunoglobulin E (IgE)-binding capacity patients with pollinosis.Native purified by multidimensional chromatography. We assessed IgE-binding using sera...
Both innate and adaptive immunity are crucial for cancer immunosurveillance, but precise therapeutic equations to restore immunosurveillance in patients with have yet be developed. In murine models, α-galactosylceramide (α-GalCer)-loaded, tumor antigen-expressing syngeneic or allogeneic cells can act as cellular adjuvants, linking the immune systems. current study, we established human artificial adjuvant vector (aAVC) consisting of HEK293 embryonic kidney stably transfected natural killer T...
The purposes of this study were to investigate the effect our recently developed truncated secretory leukoprotease inhibitor (SLPI) on bleomycin (BLM)-induced lung injury and fibrosis in hamsters, which is widely used as a model interstitial pulmonary fibrosis, assess possible involvement neutrophil elastase pathogenesis fibrosis. was prepared by administration BLM (10 mg/kg) intratracheally hamsters. SLPI administered at dose 5 or 15 mg/kg intraperitoneally twice day only for 10 d starting...
Two different allergy tests, antigen-specific immunoglobulin E quantification (IgE test) and flow cytometric analysis of proliferation peripheral lymphocytes (lymphocyte test), were performed to examine differences in allergic reactions food allergens dogs with (FA). Thirteen diagnosed as FA based on clinical findings elimination diet trials. Seven clinically canine atopic dermatitis (CAD) used a disease control group, 5 healthy negative group. In the 19 33 allergen identified using serum...
Canine atopic-like dermatitis (ALD) is suspected to be associated with food allergies, particularly those mediated by lymphocytes. In this study, 54 cases were included as ALD dogs, based on the negative IgE test results. percentage of activated cells in helper-T lymphocytes was measured flow cytometry using cultured peripheral under allergen stimulation. We observed that 49 dogs (90.7%) had positive lymphocyte reactions against one or more allergens. The most common soybean, showing results...
Lymphocyte blastogenic responses against food allergens in dogs with hypersensitivity were evaluated this study. Eleven hypersensitivity, based on elimination and oral provocation tests allergic to allergens, examined by various such as intradermal testing, antigen‐specific IgE lymphocyte responses. The number kinds of identified positive these compared the offending that found an test. In 9 (82%) 11 there was close agreement for between results test; however, little testing those test (11%...
Lymphocyte blastogenic responses against food allergens in dogs with hypersensitivity were evaluated this study. Eleven hypersensitivity, based on elimination and oral provocation tests allergic to allergens, examined by various such as intradermal testing, antigen-specific IgE lymphocyte responses. The number kinds of identified positive these compared the offending that found an test. In 9 (82%) 11 there was close agreement for between results test; however, little testing those test (11%...
Pneumocystis carinii pneumonia was diagnosed by postmortem examination of a one-year-old Cavalier King Charles Spaniel with four-week history dyspnea. Cytologic and histologic lung tissues revealed numerous P. trophozoites cysts, specific DNA detected polymerase chain reaction. The dog showed hypogammagloblinemia extremely low levels serum IgG. It considered that in this case associated an immunodeficient condition which has already been reported Miniature Dachshunds.
Using both in vivo and vitro tests, dogs with atopic dermatitis were examined for sensitization Japanese cedar(Cryptomeria japonica, CJ)pollen allergen.Ten clinical manifestation of shown to be sensitized CJ pollen based on the results intradermal skin test serum antigen−specific IgE test.In lymphocyte stimulation showed blastogenic response after crude antigen all 5 cases examined.The peripheral leukocytes increased histamine release 2 examined.These data indicate that a proportion is...