Abstract BACKGROUND Botrytis cinerea is one of the most serious plant diseases and severely threatens agricultural production. The rapidly intensifying resistance makes commercial chemical fungicides lose control efficacy. Developing new with novel structures modes action an effective measure to solve this problem. RESULTS In work, 29 sulfonamide compounds containing pinacolone scaffolds were designed synthesized based on active substructure splicing strategy. biological activity assay three...

Formononetin has been demonstrated to protect against cerebral ischemia–reperfusion injury, however its mechanism be further researched. This study examined the effect of formononetin on injury in rats using PARP-1/PARG/Iduna signaling pathway. In male SD rats, a model was developed. Animals were randomly assigned one eight groups: Sham operation, operation + formononetin, MCAO, MCAO PARP inhibitor (PJ34) PJ34 PARG (Ethacridine lactate) and ethacridine lactate formononetin. The neurological...

A health survey was conducted on 199 workers engaged in dividing and packaging pyrethroids. The subjects were exposed to fenvalerate at 0.012-0.055 mg/m3 deltamethrin 0.005-0.012 the air with simultaneous skin contact for 0.5-4.5 months. Burning sensations tightness or numbness face appeared two thirds of one third had sniffs sneezes. Abnormal facial sensations, dizziness, fatigue, miliary red papules more evident summer than winter. Neither abnormalities other organs systems nor symptoms...

Drought stress is the most harmful one among other abiotic stresses with negative impacts on crop growth and development. Drought-hardening a feasible widely used method in tobacco seedlings cultivation. It has gained extensive interests due to its role improving drought tolerance. This research aimed investigate of drought-hardening unravel multiple mechanisms underlying tolerance adaptation.This study was designed which various treatments (CK (no drought-hardening), T1 (drought-hardening...

Drought stress hinders the growth and development of crop plants ultimately its productivity. It is expected that drought will be frequent intense in future due to drastic changes global climate. necessary make more resilient through various techniques; drought-hardening one them. Defining metabolic strategies used by tobacco confer tolerance important for maintaining plant physiological functions, but studies addressing this topic are limited. This study was designed elucidate adaptation...

Type 3 resistant starch from Canna edulis (Ce-RS3) is an insoluble dietary fiber which could improve blood lipids in animals, but clinically robust evidence still lacking. We performed a double-blind randomized controlled trial to assess the effects of Ce-RS3 on mild hyperlipidemia. One hundred and fifteen patients were included followed recruitment criteria, randomly allocated receive or placebo (native edulis) for 12 weeks (20 g/day). In addition serum lipids, complete counts, inflammatory...

Abstract The chloroplast protein ferredoxin 1 (FD1), with roles in the electron transport chain, is known to interact coat proteins (CPs) of Tomato mosaic virus and Cucumber virus. However, our understanding FD1 infection remains limited. Here, we report that Potato X (PVX) p25 interacts FD1, whose mRNA levels are reduced by PVX or transient expression p25. Silencing Tobacco rattle virus-based virus-induced gene silencing (VIGS) promoted local systemic plants PVX. Use a drop-and-see (DANS)...

Chilli veinal mottle virus (ChiVMV), which belongs to the genus Potyvirus of family Potyviridae, mainly infects solanaceous plants and has caused serious economic losses in Asia Africa. Tobacco infected with ChiVMV suffered from punctate necrosis leaves, leaf deformation, systemic leaves stems, eventually plant death. However, infection could not usually be identified given lack rapid efficient detection assays tobacco plants. Therefore, an isolate tobacco-infecting (ChiVMV-LZ) was obtained,...

Pepper mild mottle virus (PMMoV) is a member in the genus Tobamovirus and infects mainly solanaceous plants. However, mechanism of virus-host interactions remains unclear. To explore responses pepper plants to PMMoV infection, we analyzed transcriptomic changes after infection using high-throughput RNA sequencing approach explored roles host autophagy regulating infection.A total 197 differentially expressed genes (DEGs) were obtained including 172 significantly up-regulated 25...

Major latex proteins (MLPs) belong to the MLP subfamily in Bet v 1 protein family and respond both biotic abiotic stresses, which play critical roles plant disease resistance. As type species of widely distributed economically devastating Potyvirus, Potato virus Y (PVY) is one major constraints important crop plants including tobacco (Nicotiana benthamiana) worldwide. Despite great losses owing PVY infection tobacco, there no previous study investigating potential role MLPs developing...

The occurrence of plant diseases is closely associated with the imbalance tissue microecological environment. regulation phyllosphere microbial communities has become a new and alternative approach to biological control foliar diseases. In this study, Bacillus velezensis SYL-3 isolated from Luzhou exhibited an effective inhibitory effect against Alternaria alternata tobacco mosaic virus (TMV). analysis microbiome by PacBio sequencing indicated that treatment significantly altered fungal...

Drought stress is one of the main factors limiting crop production, which provokes a number changes in plants at physiological, anatomical, biochemical and molecular level. To unravel various mechanisms underpinning tobacco (Nicotiana tabacum L.) drought tolerance, we conducted comprehensive transcriptome analyses three cultivars (i.e., HongHuaDaJinYuan (H), NC55 (N) Yun Yan-100 (Y)) seedlings that had been exposed to stress. As result, H maintained higher growth term less reduction plant...

Novel anti-viral natural product ε-poly-l-lysine (ε-PL) produced by Streptomyces is a homopolymer of l-lysine, which the underlying molecular mode action remains to be further elucidated. In this study, ε-PL induced significant fragmentation tobacco mosaic virus (TMV) virions and delayed systemic infection TMV-GFP as well wild-type TMV in plants. treatment also markedly inhibited RNA accumulation BY-2 protoplasts. The results RNA-seq indicated that agent significantly differential expression...

The mevalonate (MVA) pathway in eukaryotic organisms produces isoprenoids, sterols, ubiquinone, and dolichols. These molecules are vital for diverse cellular functions, ranging from signaling to membrane integrity, post-translational modification energy homeostasis. However, information on the MVA Phytophthora species is limited. In this study, we identified genes reconstructed complete sojae silico. We characterized function of P. by treatment with enzyme inhibitor lovastatin, deletion...

Microbial secondary metabolites produced by Streptomyces have diverse application prospects in the control of plant diseases. Herein, fermentation filtrate SN40 effectively inhibited infection tobacco mosaic virus (TMV) Nicotiana glutinosa and systemic potato Y (PVY) benthamiana. Additionally, metabolomic analysis indicated that anisomycin (C14H19NO4) trans-3-indoleacrylic acid (C11H9NO2) were highly abundant crude extract suppressed TMV as well PVY. Subsequently, transcriptomic was...

Obesity is a most prevalent human health problem. Several studies showed that appropriate modulation of gut microbiota could help reshape the metabolic profile obese individuals, thereby altering development obesity. A nutritional strategy for treating obesity includes prebiotics. Type 3 Resistant Starch from Canna edulis (Ce-RS3) dietary fiber exerts potential effects on intestinal microbial community; however, landscape and anti-obesity mechanism remain unclear. In present study, mice were...

Abstract This article explores augmented reality (AR) possibilities for the preservation of national cultural art, popularization Chinese musical instruments among youth, and meeting tourist interest in heritage China a fascinating interactive form. Using generated database containing information on traditional Guizhou province, authors have developed an AR application adapted educational purposes. Its central aim was to acquaint people with context familiarization culture China. Testing...

Potato virus Y (PVY) mainly infects Solanaceous crops, resulting in considerable losses the yield and quality. Iron (Fe) is involved various biological processes plants, but its roles resistance to PVY infection has not been reported. In this study, foliar application of Fe could effectively inhibit early PVY, a full-length transcriptome Illumina RNA sequencing was performed investigate modes action PVY-infected Nicotiana tabacum. The results showed that 18,074 alternative splicing variants,...

Expression of the GH3 promoter/GUS reporter (GH3/GUS) gene in transgenic tobacco is silent most vegetative tissues and organs, but can be specifically induced by auxin all cell types throughout plant, indicating that a limiting factor for activation GH3/GUS gene. In absence exogenous auxin, expression either correlates with physiological events which are presumably mediated or occurs cells where relatively high levels endogenous expected. For example, expressed dormant lateral buds, tips...

Ningnanmycin (NNM) belongs to microbial pesticides that display comprehensive antiviral activity against plant viruses. NNM treatment has been shown efficiently delay or suppress the disease symptoms caused by tobacco mosaic virus (TMV) infection in local-inoculated systemic-uninoculated leaves, respectively. However, underlying molecular mechanism of NNM-mediated remains be further elucidated. In this study, 414 differentially expressed genes (DEGs), including 383 which were up-regulated...

Histone H2B protein is not only structurally important for chromosomal DNA packaging but also involved in the regulation of gene expression, including immune response plants against pathogens. In this study, we show that potato virus X (PVX) infection resulted reduced expression at both mRNA and level