A5028188188- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- RNA modifications and cancer
- Cardiac Structural Anomalies and Repair
- Reproductive Biology and Fertility
- Congenital Diaphragmatic Hernia Studies
- Prenatal Screening and Diagnostics
- Photoreceptor and optogenetics research
- Neuroscience and Neuropharmacology Research
- Eosinophilic Esophagitis
- Immune Cell Function and Interaction
- Neural dynamics and brain function
- Renal and related cancers
- Developmental Biology and Gene Regulation
- HVDC Systems and Fault Protection
- Virus-based gene therapy research
- Animal Genetics and Reproduction
- IL-33, ST2, and ILC Pathways
- Microtubule and mitosis dynamics
Chinese Academy of Sciences
2023-2025
Center for Excellence in Brain Science and Intelligence Technology
2023-2025
State Key Laboratory of Neuroscience
2025
Shanghai Center for Brain Science and Brain-Inspired Technology
2023-2024
University of Chinese Academy of Sciences
2024
Institute of Microbiology
2024
Abstract Somatic cell nuclear transfer (SCNT) successfully clones cynomolgus monkeys, but the efficiency remains low due to a limited understanding of reprogramming mechanism. Notably, no rhesus monkey has been cloned through SCNT so far. Our study conducts comparative analysis multi-omics datasets, comparing embryos resulting from intracytoplasmic sperm injection (ICSI) with those SCNT. findings reveal widespread decrease in DNA methylation and loss imprinting maternally imprinted genes...
N
Abstract Innate lymphoid cell precursors (ILCPs) develop into distinct subsets of innate cells (ILCs) with specific functions. The epigenetic program underlying the differentiation ILCPs ILC remains poorly understood. Here, we reveal genome-wide distribution and dynamics DNA methylation hydroxymethylation in their respective precursors. Additionally, find that hydroxymethyltransferase TET1 suppresses ILC1 but not ILC2 or ILC3 differentiation. deficiency promotes by inhibiting TGF-β...
Abstract The efficiency of homology-directed repair (HDR) plays a crucial role in the development animal models and gene therapy. We demonstrate that microhomology-mediated end-joining (MMEJ) constitutes substantial proportion DNA during CRISPR-mediated editing. Using CasRx to downregulate key MMEJ factor, Polymerase Q ( Polq ), we improve targeted integration linearized fragments single-strand oligonucleotides (ssODN) mouse embryos offspring. CasRX-assisted (CATI) also leads improvements...
Cell fate determination and transition are of paramount importance in biology medicine. Naive pluripotency could be achieved by reprogramming differentiated cells. However, the mechanism is less clear. Osmolarity an essential physical factor that acts on living cells, especially for pluripotent but its significance cell remains unexplored. To investigate role osmolarity underlying mechanism. Flow cytometry, quantitative real-time PCR, teratoma chimeric mice assays were performed to assess...