Parthenocarpy is a desired trait in tomato because it can overcome problems with fruit setting under unfavorable environmental conditions. A parthenocarpic cultivar, 'MPK-1', gene, Pat-k, exhibits stable parthenocarpy that produces few seeds. Because 'MPK-1' seeds, seedlings are propagated inefficiently via cuttings. It was reported Pat-k located on chromosome 1. However, the gene had not been isolated and relationship between low seed set remained unclear. In this study, we to clarify...

Next-generation sequencing (NGS) library construction often involves using restriction enzymes to decrease genome complexity, enabling versatile polymorphism detection in plants. However, plant leaves frequently contain impurities, such as polyphenols, necessitating DNA purification before enzymatic reactions. To overcome this problem, we developed a PCR-based method for expeditious NGS preparation, offering flexibility number of detected polymorphisms. By substituting segment the simple...

Plants are exposed to high light intensity, leaf temperatures and air-to-leaf water vapor pressure deficit (ALVPD) during the day. These environmental stresses cause stomatal closure photoinhibitory damage, leading midday depression of photosynthesis. Chloroplast positioning is essential for efficient operation However, chloroplast behavior before, during, even after photosynthesis remains unknown. We investigated changes in intracellular chloroplasts photosynthetic traits under a diurnal...

MIG-seq (Multiplexed inter-simple sequence repeats genotyping by sequencing) has been developed as a low cost technology, although the number of polymorphisms obtained is assumed to be minimal, resulting in application this technique analyses agricultural plants. We applied 12 plant species that include various crops and investigated relationship between genome size bases can stably sequenced. The loci, which sequenced MIG-seq, are positively correlated. This due linkage simple (SSRs)...

'No.80' (Capsicum chinense) from the Caribbean is a valuable genetic source aspect of its non-pungent and highly aromatic traits. In present study, non-pungency, volatile components, phylogenetic origin were analyzed with another C. chinense cultivar, 'No.2' Brazil, which also but less aromatic. Expressions deduced amino acid sequences acyltransferase (Pun1) normal compared pungent 'Habanero'. Insertions 7-bp 8-bp resulting in frameshift mutations found coding regions putative...

Tomato yellow leaf curl virus (TYLCV) infections result in decreased tomato growth and reduced yields, the production is almost entirely lost if plants are infected during early growth. 'Kyo-temari' commercial name for parthenocarpic 'MPK-1', which has been vegetatively propagated distributed to local farmers Kyoto City cultivation. During winter of 2013, typical symptoms TYLCV were observed 10 cultivars, including maintained as mother stock vegetative propagation at University. When...

Parthenocarpy is a trait where fruit set and growth are triggered without pollination fertilization.In the tomato (Solanum lycopersicum L.), this considered attractive as it reduces cost labor requirements for setting.In study, we investigated inheritance of parthenocarpy in 'MPK-1'-a parthenocarpic cultivar derived from cross between variant self-fertilization posterity 'Severianin', which exhibited strong non-parthenocarpic cultivar.It was reported that 'MPK-1' contains pat-2 gene because...

Mesophyll (M) chloroplasts in finger millet are known to aggregate the bundle sheath side when leaves constantly irradiated with extremely high-intensity light. This aggregative movement of M is also observed natural environment, but whether a light regime effective inducing response remains unclear. Abscisic acid reported trigger not only stomatal closure, photosynthetic responses accompanying remain unknown.We investigated changes chloroplast positioning and traits under diurnal patterns...

'Kyo-temari', a parthenocarpic tomato cultivar with the pat-2 gene, produces only few seeds. To elucidate cause of seed formation restriction in we observed pollen tube elongation and ovule morphology some tomatoes. In 'Renaissance', was inhibited at style base; however, tubes elongated normally but did not enter ovules. The percentage ovules which entered 70.4% 'Louis 60', 0.8% 5.1% 'Renaissance'. From observing transverse sections 'Kyo-temari' ovules, there were many an abnormal micropyle....

The participation of O-methyltransferase (COMT) in phenylpropanoid-mediated capsaicinoid biosynthesis has long been proposed. Ferulic acid, a phenylpropanoid intermediate, is precursor and produced from caffeic acid by the action COMT. As previously reported that silencing Comt expression caused drastic decrease accumulation, it was presumed loss-of-function mutation would cause loss pungency Capsicum. This hypothesis tested cloning Comt1 Comt2 placenta tissue pungent cultivar Habanero....

In pat-2 parthenocarpic tomatoes (Solanum lycopersicum L.), inhibition of seed production prevents their breeding and development. Low set is thought to be caused by a pleiotropic effect the gene because number seeds from tomato cultivars generally low; however, research on relationship between low lacking. this study, clarify develop method for promoting in tomatoes, we examined aminoethoxyvinylglycine (AVG), an indole-3-acetic acid (IAA) biosynthesis inhibitor, 'Renaissance', cultivar. F2...

Parthenocarpy is a trait in which fruit set and development are induced without fertilization, thereby contributing to labor saving production. In Cucurbita species, parthenocarpic cultivars have been reported pepo, but not maxima moschata. this regard, the identification of clarification their characteristics will contribute greatly research breeding species. study, we aimed genetically screen diverse accessions for high ability elucidate discovered by screening. We screened 33 from squash...

An understanding of the genetic mechanism underlying capsaicinoid biosynthesis is important for breeding both pungent and non-pungent peppers. Although Capsicum one earliest domesticated plant genera, only mutations in acyltransferase (Pun1) putative aminotransferase (p-AMT) have been reported as causes loss pungency. ‘No.3341’ (C. chinense) from Bolivia a cultivar. To determine reasons non-pungency ‘No.3341’, its expression levels deduced amino acid sequences Pun1 p-AMT were analyzed. In...

Histological investigations of the fruit abscission zone and morphological changes in cells ponkan (C. reticulata Blanco), hyuganatsu tamurana hort. ex Tanaka), ‘Kiyomi’ unshiu × C. sinensis (L.) Osbeck), satsuma mandarin (Citrus Marcow.) were conducted using detached fruits incubated agar medium under 25°C, 4 weeks after anthesis during secondary physiological drop. In 96 h induction by detaching fruits, cumulative ratio was 100% ponkan, 22% ‘Kiyomi’, below 10% mandarin. Fruit began at 36...

Artificially occurring parthenocarpy can be induced by exogenous application of plant hormones and is useful in the fruit production many crops trees. In cucumber (Cucumis sativus L.), a model species Cucurbitaceae family, auxin, gibberellin, cytokinin, brassinosteroids are known to induce parthenocarpy. tropical squash (Cucurbita moschata synthetic auxins parthenocarpy, but effects still unknown. addition, there few published reports on quality parthenocarpic fruits squash, these remain...

Parthenocarpy is a phenomenon that induces fruit set and development without pollination fertilization. In tomato (Solanum lycopersicum L.) cultivation, parthenocarpy considered to be an attractive trait, as it reduces the financial labor costs involved in set. Parthenocarpic genes pat-2 pat-k are used source of parthenocarpic cultivars Japan. However, effect pat-2, pat-k, their combined on vegetative traits poorly understood, even though they important for breeding cultivars. this study, we...

A new method of inducing fruit abscission by incubating detached ovaries and fruits in agar medium was developed citrus. Ovary the satsuma mandarin, ‘Kiyomi’, hyuganatsu, ponkan during early physiological drop characterized using this method. For primary drop, could be divided into three patterns, including an type a late mandarin ponkan. The cumulative ratio four species cultivars over 80% at 96 h after treatment. However, for secondary initiation earliest latest mandarin. highest 84%...

Physiological fruit drop in citrus is the deeper problem for production and many researches have suggested that decreasing of carbohydrate translocation to involved abscission. The abscission process requires 3 stages successful abscission, which include “induction”, “decision”, “execution”. However, there almost no information molecular mechanism secondary physiological citrus. Here, anatomical observation microarray analysis demonstrated candidate genes after induction by incubating...

本研究では，光透過性を有する有機薄膜型太陽電池（OPV）の設置がトマトの生育と果実生産に及ぼす影響を明らかにすることを目的とし，OPVを設置した温室（OPV温室）と設置していない温室（対照温室）でトマトの果実の収量と品質，植物体の生育量および光合成関連形質を調査した．調査は秋冬栽培と春夏栽培で実施し，秋冬栽培では86台，春夏栽培では106台のOPVパネルをそれぞれOPV温室内側に設置した．秋冬栽培と春夏栽培の両方でOPV温室内の日射量は対照温室に比べ減少した．両栽培において収量では対照温室とOPV温室の間に有意な差は認められなかったものの，OPV温室の果実乾物重は日射量の少ない秋冬栽培で対照温室に比べ23％減少した．また，秋冬栽培と春夏栽培で両温室の植物体の生育量と光合成関連形質を調査した結果，光合成関連形質は両栽培においてOPV温室でほぼ同様に低下したのに対し，植物体の生育量は果実乾物重と同様に秋冬栽培で大きく減少した．これらの結果より，OPVパネルの設置が果実乾物重と植物体に及ぼす影響は特に日射量の少ない時期で大きくなることが明らかとなった．また，本研究に用いたOPVパネルの...

単為結果性トマト品種‘京てまり’の冬季無加温栽培の収量と品質の向上を目的に，新たに開発されたポリビニールアルコールフィルムとポリクロスを貼り合わせた厚さ25 μmのフィルム（以下PVAとする）を用い，無加温プラスティックフィルムハウス内にトンネル状に被覆して，果実生産に及ぼす影響を2011年10月～2012年3月まで調査した．トンネルの被覆資材の対照として，市販の塩化ビニールフィルム（以下PVCとする）を用いた．PVAは，PVCと比較して，2,500～25,000...