Osteoarthritis (OA) is represented by the accumulation and adhesion of M1 macrophages into synovium tissues in joint microenvironment subsequent inflammatory response. Cordycerebroside A, a cerebroside compound isolated from Cordyceps militaris, exhibits anti-inflammatory activity, but has not yet been examined during OA disease. Our results indicate higher expression macrophage markers tissue patients compared with normal healthy controls. Records Gene Expression Omnibus (GEO) data set our...

Tumor malignancy is associated with several features such as proliferation ability and frequency of metastasis. Osteopontin (OPN), which abundantly expressed in bone matrix, involved cell adhesion, migration, invasion via interaction its receptor, that is, alphavbeta3 integrin. However, the effect OPN on migration activity human chondrosarcoma cells mostly unknown. Here we found increased expression matrix metalloproteinase (MMP)-9 (JJ012 cells). RGD peptide, monoclonal antibody MAPK kinase...

Abstract CCL5 (previously called RANTES) is in the CC‐chemokine family and plays a crucial role migration metastasis of human cancer cells. On other hand, effect mediated via CCR receptor. RT‐PCR flow cytometry studies demonstrated CCR5 but not CCR1 CCR3 mRNA oral cell lines, especially higher those with high invasiveness (SCC4) as compared lower levels HSC3 cells SCC9 Stimulation directly increased metalloproteinase‐9 (MMP‐9) production. MMP‐9 small interfering RNA inhibited CCL5‐induced...

Osteosarcoma, the most common primary malignant bone tumor, shows potent capacity for local invasion and distant metastasis. Connective tissue growth factor (CTGF/CCN2), a secreted protein, binds to integrins, modulates invasive behavior of certain human cancer cells. Effect CTGF in metastasis osteosarcoma is unknown. We found overexpression increasing matrix metalloproteinases (MMPs)-2 MMP-3 expression as well promoting cell migration. MicroRNA (miRNA) analysis CTGF-overexpressed versus...

Abstract Objective CCL5 (RANTES) was originally identified as a product of activated T cells and plays crucial role in the inflammatory response. This study undertaken to investigate intracellular signaling pathways involved CCL5‐induced interleukin‐6 (IL‐6) production human synovial fibroblasts. Methods CCL5‐mediated IL‐6 expression assessed by quantitative polymerase chain reaction enzyme‐linked immunosorbent assay. The mechanisms action different were studied using Western blotting....

Angiogenesis is the formation of new capillaries from preexisting vasculature. The perpetuation angiogenesis plays a critical role in pathogenesis various disease states including rheumatoid arthritis (RA). Cysteine-rich 61 (Cyr61 or CCN1) an important proinflammatory cytokine RA. Here, we investigated CCN1 associated with vascular endothelial growth factor (VEGF) production and osteoblasts. We found higher expression VEGF synovial fluid RA patients compared healthy controls. induced...

Abstract Endothelial progenitor cells (EPCs) promote angiogenesis and are therefore key contributors to a wide variety of angiogenesis-related autoimmune diseases such as rheumatoid arthritis (RA). However, the signaling mechanisms through which these influence RA pathogenesis remain unknown. The aim this study was examine whether resistin plays role in associated with by circulating EPCs. We found that levels synovial fluid tissue from patients mice collagen-induced were overexpressed...

Abstract The pro‐inflammatory cytokine interleukin 1 beta (IL‐1β) plays a critical role in osteoarthritis (OA) disease pathogenesis. MicroRNA (miRNA) activity is related to inflammation OA and some miRNAs specifically regulate IL‐mediated degradation of cartilage type II collagen. Previous studies have indicated that miR‐144‐3p useful target the regulation cytokines different diseases. However, unclear. In this study, we observed negative correlation between IL‐1β expression OA. mimic...

Abstract 2‐Methoxyestradiol (2ME) is an endogenous metabolite with estrogen receptor‐independent anti‐tumor activity. The current study seeks to determine the mechanism of activity 2ME on human chondrosarcoma. caused a time‐ and dose‐dependent cytotoxity in chondrosarcoma cells, while primary chondrocytes were minimally affected. Cells accumulated G0/G1 phase response DAPI stain indicated induction apoptosis. Bax, Cytochrome C, Caspase‐3 protein expression increased, p53 was decreased. A...

Background Connective tissue growth factor (CTGF; also known as CCN2) is an inflammatory mediator, and shows elevated levels in regions of severe injury diseases. CTGF abundantly expressed osteoarthritis (OA). However, the relationship between IL-6 OA synovial fibroblasts (OASFs) mostly unknown. Methodology/Principal Findings OASFs showed significant expression CTGF, was higher than normal SFs. stimulation with induced concentration-dependent increases expression. mediated production...

Accumulating evidence indicates that subchondral bone might play an essential role in rheumatoid arthritis (RA). Osteopontin (OPN) induces the production of important proinflammatory cytokine involved pathogenesis RA. This study evaluated activation oncostatin M (OSM) by OPN human primary osteoblasts to understand RA and characterized intracellular signaling pathways this activation. Quantitative PCR, ELISA, Western blot results indicated stimulation with OSM expression through αvβ3...

YKL-40, also known as human cartilage glycoprotein-39 or chitinase-3-like-1, is a pro-inflammatory protein that highly expressed in rheumatoid arthritis (RA) patients. Angiogenesis critical step the pathogenesis of RA, promoting infiltration inflammatory cells into joints and providing oxygen nutrients to RA pannus. In this study, we examined effects YKL-40 production cytokine interleukin-18 (IL-18), stimulation angiogenesis accumulation osteoblasts. We observed induces IL-18 osteoblasts...

Rheumatoid arthritis (RA) is characterised by the infiltration of a number proinflammatory cytokines into synovial fluid. Soya-cerebroside, an extract from Cordyceps militaris, inhibits inflammation and reduces cartilage damage in osteoarthritis model, although role soya-cerebroside inflammatory cytokine expression RA uncertain. In this study, Gene Expression Omnibus (GEO) database records revealed higher levels interleukin (IL)-1β, IL-6 IL-8 tissue compared with normal tissue. Elevated...

Chemokine (C-C motif) ligand 3 (CCL3), also known as macrophage inflammatory protein-1α, is a cytokine involved in inflammation and activation of polymorphonuclear leukocytes. CCL3 has been detected infiltrating cells tumor cells. Chondrosarcoma highly malignant that causes distant metastasis. However, the effect on human chondrosarcoma metastasis still unknown. Here, we found increased cellular migration expression matrix metalloproteinase (MMP)-2 Pre-treatment with MMP-2 inhibitor or...

Rheumatoid arthritis (RA), a common autoimmune disorder, is associated with chronic inflammatory response and unbalanced bone metabolism within the articular microenvironment. Adiponectin, an adipokine secreted by adipocytes, involved in multiple functions, including lipid pro-inflammatory activity. However, mechanism of adiponectin performance arthritic inflammation remains unclear. In this study, we observed effect on expression oncostatin M (OSM), cytokine, human osteoblastic cells....

Cysteine-rich 61 (Cyr61 or CCN1), a secreted protein from the CCN family, is an important proinflammatory cytokine. Migration and infiltration of mononuclear cells to inflammatory sites play critical role in pathogenesis rheumatoid arthritis (RA). Monocyte chemoattractant protein-1 (MCP-1/CCL2) key chemokine that regulates migration monocytes. Here, we examined CCN1 monocyte migration, CCL2 expression osteoblasts. We found higher levels synovial fluid RA patients compared with non-RA...

Hepatocellular carcinoma (HCC) is a liver malignancy and major cause of cancer mortality worldwide. AURKA (aurora kinase A) mitotic serine/threonine that functions as an oncogene plays critical role in hepatocarcinogenesis. We report on the association between 4 single nucleotide polymorphisms (SNPs) gene (rs1047972, rs2273535, rs2064836, rs6024836) HCC susceptibility well clinical outcomes 312 patients with 624 cancer-free controls. found carriers TT allele variant rs1047972 were at greater...

Osteoarthritis (OA) is characterized by the infiltration and adhesion of monocyte into joint synovium. Vascular cell molecule 1 (VCAM-1) a critical that controls motility during OA progression. Cordycerebroside A, cerebroside compound isolated from Cordyceps militaris, inhibits production inducible nitric oxide synthase (iNOS) cyclooxygenase-2 (COX-2) in synovial macrophages, but has not yet been investigated OA. Gene Expression Omnibus (GEO) dataset analysis revealed higher levels VCAM-1...

Fat embolism syndrome (FES) is a potentially fatal complication of long bone fractures. There have been no reports FES in fractures this decade Taiwan. The purpose study was to review the experiences tertiary referral center between January 1997 and February 2008.Between 2008, 13 patients with documented our institution were reviewed. diagnosed clinically by at least 2 major criteria or 1 4 minor signs Gurd's criteria.The incidences FES, less than those reported literature, 0.15% fracture...