A5083738577- Periodontal Regeneration and Treatments
- Dental Implant Techniques and Outcomes
- Dental Radiography and Imaging
- Oral microbiology and periodontitis research
- dental development and anomalies
- Dental Trauma and Treatments
- Oral and Maxillofacial Pathology
- Temporomandibular Joint Disorders
- Endodontics and Root Canal Treatments
- Bone and Dental Protein Studies
- Dental Health and Care Utilization
- Rheology and Fluid Dynamics Studies
- Oral and gingival health research
- Bone Tissue Engineering Materials
- Melanoma and MAPK Pathways
- Cancer Immunotherapy and Biomarkers
- Engineering Applied Research
- Facial Trauma and Fracture Management
- Aluminum Alloy Microstructure Properties
- Salivary Gland Disorders and Functions
- Intellectual Property and Patents
- Bone health and treatments
- Streptococcal Infections and Treatments
- Advanced Polymer Synthesis and Characterization
- CAR-T cell therapy research
Fujifilm (Japan)
2024
Nihon Bunka University
2024
Nihon University
1995-2024
Nagoya City University
2019-2024
Tokyo Dental College
2011-2023
The Open University of Japan
2023
Japan Petroleum Energy Center
2023
Kanagawa Dental University
2022
Kasugai Municipal Hospital
2022
Tokyo Medical and Dental University
1963-2020
In the oral and maxillofacial surgery dentistry fields, use of three-dimensional (3D) patient-specific organ models is increasing, which has increased cost obtaining them. We developed an environment in our facility we can design, fabricate, 3D called "One-stop printing lab". The lab made it possible to quickly inexpensively produce that are indispensable for surgery. report model fabrication after determining dimensional accuracy with different laminating pitches (; layer thickness)...
We recently reported a new method to purify the induced pluripotent stem (iPS)-derived osteoprogenitors (iPSop). In this paper, we optimized procedure and characterized cells at each process step. observed that 10 days of treatment with FGF-2, IGF-1 TGF-β (FIT) resulted in early-phase osteoblasts 14 late-phase osteoblasts. found 1,25(OH)2 vitamin D3 increased expression osteocalcin decreased tissue-non-specific alkaline phosphatase runt-related transcription factor 2 (RUNX2) iPSop-day14...
Anti-programmed cell death 1 antibodies (anti-PD-1 Abs) are widely used for advanced melanoma, but the efficacy of an anti-PD-1 Abs is limited in Asian population. There remains unmet need to improve therapeutic effects treatment, particularly melanoma patients who refractory Abs. The aim was evaluate treatment combination with TM5614 (plasminogen activator inhibitor-1: PAI-1 inhibitor) unresectable melanoma.
Background Enhanced glucose metabolism has been reported in many cancers. Glucose-6-phosphate dehydrogenase (G6PD) is a rate-limiting enzyme involved the pentose phosphate pathway, which maintains NADPH levels and protects cells from oxidative damage. We recently found that low G6PD expression correlates with active tumor immunity. However, mechanism involving immunity remained unclear. Methods conducted vitro studies using G6PD-knocked down malignant melanoma cells, pathway analysis GEO...
Abstract Sera from a number of patients with periodontal diseases were shown to have specific immunoglobulin G (IgG) antibodies against fimbriae and membrane proteins Bacteroides gingivalis , suspected pathogen, by using Western blotting analysis. The sera had strong tendency react fimbriae, or exactly oligomeric structures native β ‐structure rich‐conformation. However, the did not fimbrilin, constituent protein which is denatured sodium dodecyl sulfate.
Anti-PD-1-based immunotherapy is considered a preferred first-line treatment for advanced BRAF V600-mutant melanoma. However, recent international multi-center study suggested that the efficacy of poorer in Asian patients non-acral cutaneous subtype. We hypothesized optimal may be different.We retrospectively collected data with melanoma treated BRAF/MEK inhibitors (BRAF/MEKi), anti-PD-1 monotherapy (Anti-PD-1), and nivolumab plus ipilimumab (PD-1/CTLA-4) between 2016 2021 from 28...
Abstract— The extent of infraposition replanted and subsequently ankylosed permanent incisors was examined in a longitudinal study 52 patients. Study cast models were made during the follow‐up period. Infraposition evaluated on frontal photos taken parallel with occlusal plane. defined as difference between position incisal edge incisor adjacent non‐injured apicocoronal direction, measured digital caliper at 50 X magnification negatives. precision this measuring procedure 2.9% accuracy 2.0%....
The purpose of these studies was to investigate the effects melatonin on production steroids (progesterone, testosterone, and estradiol) cAMP by preovulatory follicles examine changes in concentrations ovary during estrous cycle. Adult cyclic hamsters were used this study. Melatonin ovary, pineal gland, serum measured at mid-light mid-dark Effects steroidogenesis follicles, thecae, granulosa cells examined, its effect also investigated. (0.1-10 ng/ml) had no steroid absence hCG, but...
A 75-kilodalton major protein (75K protein) was purified to homogeneity from the cell lysate fraction and envelope of Bacteroides gingivalis 381. The 75K originally present in outer membrane or outermost part this organism as a large, stable complex with an apparent molecular weight about 2,000,000. Heating at 80 degrees C higher temperatures presence sodium dodecyl sulfate needed completely dissociate it monomers. Amino acid analysis revealed that had 50% nonpolar amino acids. Various...
The objective of this study was to three-dimensionally observe the morphological characteristics mesiobuccal root canals Japanese maxillary first molars using microcomputed tomography (Micro-CT) and classify canal variations. This used 90 molars. Three-dimensional reconstruction performed data obtained by Micro-CT, cross-sections were observed. Moreover, morphology classified configuration diameter, evaluated for occurrence classification Weine et al. (1969) as a reference. Overall, single...
In this study, we developed a new method to stimulate osteogenic differentiation in tissue-nonspecific alkaline phosphatase (TNAP)-positive cells liberated from human induced pluripotent stem (hiPSCs)-derived embryoid bodies (EBs) with 14 days long TGF-β/IGF-1/FGF-2 treatment. TNAP is marker protein of osteolineage cells. We analyzed and isolated TNAP-positive E-cadherin-negative nonepithelial by fluorescence-activated cell sorting. Treating the combination transforming growth factor...
Here, we describe a case of autotransplantation mandibular horizontally impacted third molar using 3-dimensional (3D) model based on limited cone-beam computed tomography (CBCT) images for diagnosis, 3D morphological evaluation, preoperative treatment planning, and surgical simulation. A 27-year-old woman visited this hospital conservative the left second molar. Intraoral radiography CBCT revealed C-shaped root canal in mesial root, compressive resorption distal due to impingement crown...
Previously, we demonstrated that the inductive properties of bone morphogenetic protein (BMP) highly depend on nature carrier material used for implantation. In this paper, show administration BMP incorporated in a fibrous collagen membrane can help to regenerate periodontal ligament and cementum both cat canines monkey molars. The partially purified bovine was combined with one or two layers membrane. Although single layer approach showed partial regeneration defects, it also quite often...
Flanking DNA regions of the fimbrilin gene (designated fimA), which encodes major subunit protein Porphyromonas (Bacteroides) gingivalis fimbriae, were cloned in several manners from P. chromosome into Escherichia coli by screening with probes derived a 2.5-kb SacI fragment previously cloned. A total 10.4 kb fragments genome was pUC plasmid. Expression fimA and possible flanking genes examined plasmid vector system bacteriophage T7 RNA polymerase-promoter expression system. The results show...
Abstract – Vertical root fractures lead to advanced periodontal breakdown with deep pockets and vertical bone defects. The purpose of this study is evaluate clinically the healing fracture treatment using adhesive resin cement. In 22 patients, 23 teeth were treated 4‐META/MMA‐TBB Eleven fractured roots bonded through canal (group A) 12 extra‐orally replanted B). All then restored full cast crowns ( n =20) or coping =3). Mean probing depth was 6.6 mm at pre‐treatment 4.4 6 months after in...
Periodontal regeneration under application of bone morphogenetic protein (BMP) is compromised by ankylosis. Ankylosis disappearance following BMP has been observed in the case a small defect, which might be beneficial change for periodontal regeneration. However, histological observation ankylosis not demonstrated large defect. The purpose this present study was to confirm resolution during recombinant human BMP-2 (rhBMP-2) applied class III furcation defects.Class defects were created...
Runt-related transcription factor 2 (RUNX2) haploinsufficiency causes cleidocranial dysplasia (CCD) which is characterized by supernumerary teeth, short stature, clavicular dysplasia, and osteoporosis. At present, as a therapeutic strategy for osteoporosis, mesenchymal stem cell (MSC) transplantation therapy performed in addition to drug therapy. However, MSC-based osteoporosis CCD patients difficult due reduction the ability of MSCs differentiate into osteoblasts resulting from impaired...
The purpose of this study is to investigate the early responses human periodontal ligament cells attached recombinant platelet‐derived growth factor‐BB and bone morphogenetic protein‐2 applied EDTA‐demineralized dentin. One hundred seventy‐four root‐planed flat dentin blocks were prepared from mid‐third periodontally diseased tooth roots. After demineralization with 24% EDTA (pH 7.02) 120 treated 0.5 1 μg/ml rhPDGF‐BB, 3 rhBMP‐2 only MEM as control (24/group). Human (HPLC) seeded on these...
Background: To demonstrate the clinical characteristics of patients with cervical cord injury (CCI) without bony in Japan. Methods: Retrospective review 127 CCI treated between January 2003 and October 2005 at 11 institutions. Results: Prevalence was 32.2% among all CCIs 0.81% blunt traumas. Mean age 60.4 years (range, 19–90 years), 104 (82%) ≥46 old (older group). The major mechanism younger (<46 years) traffic injuries (39%), whereas minor falls (44%) predominated older patients....
Supernumerary teeth in the molar area are classified as paramolars or distomolars based on location. They occur frequently maxilla, but only rarely mandible. These fused with adjacent teeth. When this occurs, pulp cavities may also be connected. This makes diagnosis and planning of endodontic treatment extremely difficult. Here we report a case mandibular second paramolar, necessitating dental treatment. Intraoral panoramic radiographs were obtained for an evaluation diagnosis. Although...
This study investigated the effect of a high ratio fresh frozen plasma (FFP) to red blood cells (RBCs) within first 6 and 24 h after admission on mortality in patients with severe, blunt trauma.This retrospective observational included 189 trauma an Injury Severity Score (ISS) ≥16 requiring RBC transfusions h. Receiver operating characteristic (ROC) curve analysis was performed calculate cut-off values FFP/RBC for outcome. The were then divided into two groups according value. Patient...
Abstract The effects of three cationic surfactants, dodecylammonium chloride (DAC), dodecyldimethylammonium (DDAC), and (DTDAC), on the conformation poly( L ‐glutamic acid) at neutral pH were examined by CD. maximum extent α‐helix induction occurs for each surfactant when mixing ration is about unity. Different specific to surfactant, as described below, appear in range ratios larger than that required induction. In case DTAC, α‐helices disintegrate into random coils. DDAC, aggregation takes...