A5101800224- Gut microbiota and health
- Microbial Community Ecology and Physiology
- Asthma and respiratory diseases
- Genomics and Phylogenetic Studies
- Probiotics and Fermented Foods
- Renal Transplantation Outcomes and Treatments
- Microbial Metabolic Engineering and Bioproduction
- Polyomavirus and related diseases
- MicroRNA in disease regulation
- Transplantation: Methods and Outcomes
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- RNA modifications and cancer
- Microtubule and mitosis dynamics
- IL-33, ST2, and ILC Pathways
- Clostridium difficile and Clostridium perfringens research
- Immune cells in cancer
- Protist diversity and phylogeny
- Bacteriophages and microbial interactions
- Cancer-related gene regulation
- Enzyme Structure and Function
- Species Distribution and Climate Change
- Sphingolipid Metabolism and Signaling
- Antenna Design and Analysis
- Advanced Proteomics Techniques and Applications
- Circular RNAs in diseases
University of Massachusetts Amherst
2020-2025
University of Illinois Chicago
2012-2020
Institute of Genomics and Integrative Biology
2010-2018
Council of Scientific and Industrial Research
2010-2018
Rationale: Bioactive lipid mediators, derived from membrane precursors, are released into the airway and airspace where they bind high-affinity cognate receptors may mediate asthma pathogenesis. Lysophosphatidic acid (LPA), a bioactive mediator generated by enzymatic activity of extracellular autotaxin (ATX), binds LPA receptors, resulting in an array biological actions on cell proliferation, migration, survival, differentiation, motility, therefore could pathogenesis.Objectives: To define...
Abstract Macrophages are a heterogeneous population of immune cells that essential for the initiation and containment inflammation. There 2 well-established populations inflammatory macrophages: classically activated M1 alternatively M2 macrophages. The FoxO family transcription factors plays key roles in number cellular processes, including cell growth, metabolism, survival, In this study, we determined whether expression FoxO1 contributes polarization macrophages toward M2-like phenotype...
Abstract Serotonin transporter (SERT) plays a critical role in regulating extracellular availability of serotonin (5-HT) the gut and brain. Mice with deletion SERT develop metabolic syndrome as they age. Changes microbiota are being increasingly implicated Metabolic Syndrome Diabetes. To investigate relationship between microbiome SERT, this study assessed fecal cecal profile 11 to 12 week-old +/+ −/− mice. Microbial DNA was isolated, processed for metagenomics shotgun sequencing, taxonomic...
Abstract Recent studies have established that the human urine contains a complex microbiome, including virome about which little is known. Following immunosuppression in kidney transplant patients, BK polyomavirus (BKV) has been shown to induce nephropathy (BKVN), decreasing graft survival. In this study we investigated profile of BKV+ and BKV− recipients. Virus-like particles were stained confirm presence VLP samples. Metagenomic DNA was purified, analyzed using metagenomic shotgun...
Diverse eukaryotic cells assemble microtubule networks that vary in structure and composition. While we understand how build with specialized functions, do not know diversify across deep evolutionary timescales. This problem has remained unresolved because most organisms use shared pools of tubulins for multiple networks, making it impossible to trace the evolution any single network. In contrast, amoeboflagellate
Abstract Aim Aging decreases the metabolic rate and increases risk of diseases, highlighting need for alternative strategies to improve health. Heat treatment (HT) has shown various benefits, but its ability counteract aging‐associated slowdown remains unclear. This study aimed investigate impact whole‐body HT on energy metabolism, explore potential mechanism involving heat sensor TRPV1, examine modulation gut microbiota. Methods Ten‐month‐old female C57BL/6 mice a high‐fat (HF) diet (45%...
Abstract Reactive oxygen species (ROS) generated by NADPH oxidase are generally known to be proinflammatory, and it seems counterintuitive that ROS play a critical role in regulating the resolution of inflammatory response. However, we observed deficiency p47phox component macrophages was associated with paradoxical accentuation inflammation whole animal model noninfectious sepsis induced endotoxin. We have confirmed this observation interrogating four separate vivo models use complementary...
The role of different lineage specific transcription factors in directing hematopoietic cell fate towards myeloid is well established but the status epigenetic modifications has not been defined during this important developmental process. We used non proliferating, PU.1 inducible progenitor cells and differentiating bone marrow derived macrophages to study dependent KLF4 transcriptional regulation its promoter demethylation monocyte/macrophage differentiation. Expression was regulated by...
Background Differences in asthma severity may be related to inflammation the airways. The lower airway microbiota has been associated with clinical features such as obstruction, symptom control, and response corticosteroids. Objective To assess relationship between local inflammation, of disease, atopic asthmatics. Methods A cohort young adult, asthmatics intermittent or mild/moderate persistent symptoms (n = 13) were assessed via bronchoscopy, lavage, spirometry. These individuals compared...
The production of microRNAs (miRNA) is influenced by various stimuli, including environmental stresses. We hypothesized that reactive oxygen species (ROS)-associated stress could regulate macrophage miRNA synthesis. miRNAs undergo unique steps maturation processing through either one two pathways cytoplasmic processing. Unlike the canonical pathway, regulation alternative has not been fully elucidated yet. cultured bone marrow derived macrophages (BMDM) from wild type (WT) and p47(phox-/-)...
Abstract The microbiome of dental clinic wastewater and its impact on mercury methylation remains largely unknown. Waste generated during procedures enters the sewer system contributes a significant fraction total (tHg) methyl (MeHg) load to treatment facilities. Investigating influence geochemical factors structure is critical step linking methylating microorganisms in (DWW) ecosystems. DWW samples from were collected over eight weeks analyzed for parameters, tHg, MeHg bacterio-toxic heavy...
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It is well accepted that dysbiosis of microbiota associated with disease; however, the biological mechanisms promote susceptibility or resilience to disease remain elusive. One major limitations previous microbiome studies has been lack complementary metatranscriptomic (functional) data complement interpretation metagenomics (bacterial abundance). The purpose this study was twofold, first evaluate bacterial diversity and differential gene expression gut using shotgun (MG) metatranscriptomics...
Hemozoin (Hz) is released from ruptured erythrocytes during malaria infection caused by Plasmodium sp., in addition the infected individuals are prone to bacterial sepsis. The molecular interactions between Hz, components and macrophages remains poorly investigated. In this report, we investigated combinatorial immune-modulatory effects of phagocytosed Interferon gamma (IFNγ) or lipopolysaccharide (LPS) macrophages. Macrophages were treated with various concentrations commercial synthetic...
We report here the complete genome sequence of polyomavirus BK subtype Ib-1, isolate AR11, identified in urine from a human kidney transplant recipient with clinical diagnosis viremia. The AR11 is closely related to reference strain 1 J2B-2 99% identity.