Abstract Extracellular vesicles (EVs) derived from human adipose-derived stem cells (hADSCs) possess the proangiogenic potential for ischaemic diseases. Thus, our study aimed to evaluate therapeutic effects of hADSC-EVs on fat grafting and explore mechanism promoting angiogenesis. The EVs released by hADSCs incubated under normal or hypoxic conditions were employed supplement in a nude mouse model. proliferation, migration, tube formation vascular endothelial growth factor (VEGF) secretion...

Background Preventing scar formation during wound healing has important clinical implications. Numerous studies have indicated that adipose-derived stem cell culture mediums, which are rich in cytokines and extracellular vesicles (EVs), regulate matrix remodeling prevent after healing. Therefore, using a rabbit model, we tried to demonstrate factor mediums plays major role preventing (EVs or cytokines), as well revealing the underlying mechanism. Methods Human cells (hASCs) were isolated...

Cyclic diguanosine monophosphate (c-di-GMP) is a second messenger that plays crucial role in regulating biofilm development, yet the Gram-positive bacteria remains elusive. Here, we demonstrated dispersed cells from biofilms of Bacillus velezensis FZB42 exhibit unique phenotype and gene expression compared to planktonic cells. Transcriptomic analysis revealed 1327 downregulated 1298 upregulated genes, among which c-di-GMP phosphodiesterase coding yuxH was remarkably upregulated. Deletion led...

Background: Growing evidence has demonstrated that adipose-derived stem cell-derived extracellular vesicles enhance the survival of fat grafts and browning white adipose tissue. We evaluated whether supplementation with promotes grafts. Methods: Extracellular derived from cells were injected into C57BL/6 mice once per week until postgraft 12. The collected weighed after weeks 2, 4, histological morphology, neovascularization, proportion M2 macrophages evaluated. ability to promote macrophage...

To investigate the effects of adipose-derived stem cell released exosomes (ADSC-Exos) on wound healing in diabetic mice.The ADSCs were isolated from adipose tissue donated by patients and cultured enzymatic digestion. The supernatant 3rd generation was used to extract Exos (ADSC-Exos). morphology ADSC-Exos observed transmission electron microscopy. membrane-labeled proteins (Alix CD63) detected Western blot, particle size distribution nanoparticle tracking analyzer. fibroblasts skin 5th with...

Osteogenesis and angiogenesis are tightly coupled processes during bone development formation. It is thus well known that the enhancement of vascularization great importance in tissue engineering. As a potential approach for repairing defects, constructs should therefore replicate essential components vivo microenvironments to promote cell osteogenic differentiation while at same time induce angiogenic response. In light standpoint above, combination human bone-derived scaffolds BMSCs...

Currently, there is a lack in therapy that promotes the reepithelialization of diabetic wounds as an alternative to skin grafting. Here, authors hypothesized extracellular vesicles from adipose-derived stem cells (ADSC-EVs) could accelerate wound closure through rescuing function keratinocytes mice.The effect ADSC-EVs on biological human keratinocyte was assayed vitro. In vivo, 81 male severe combined immune deficiency mice aged 8 weeks were divided randomly into vesicle-treated diabetes...

Subcutaneous transplantation of decellularized adipose tissue was capable recellularization during soft repair. However, further improvements are required to promote angiogenesis and adipogenesis. Here, the authors proposed a neo-mechanical protocol isolate tissue-derived extracellular vesicles (ATEVs) through lipoaspirate as mediator for both adipogenesis, prepared ATEV-rich hydrogel engineering.Adipose liquid extract lipid-devoid were extracted homogenization repeated freeze thaw cycles....

To investigate the effect of adipose-derived stem cell derived exosomes (ADSC-Exos) on angiogenesis after skin flap transplantation in rats.ADSCs were isolated and cultured by enzymatic digestion from voluntary donated adipose tissue patients undergoing liposuction. The 3rd generation cells observed under microscopy identified flow cytometry oil red O staining at 14 days induction adipogenesis. After as ADSCs, ADSC-Exos was extracted density gradient centrifugation. And morphology...

Background Random flaps are widely used for wound repair. However, flap necrosis is a serious complication leading to the failure of operation. Our previous study demonstrated great proangiogenic potential hypoxia-treated adipose-derived stem cells–extracellular vesicles (HT-ASC-EVs). Thus, we aim evaluate effect HT-ASC-EVs in survival and angiogenesis random skin rats. Methods Adipose-derived were respectively isolated from cell culture medium 3 donors via ultracentrifugation. The...

The healing of diabetic wounds is poor due to a collagen deposition disorder. Matrix metalloproteinase-9 (MMP-9) closely related in the process tissue repair. Many studies have demonstrated that extracellular vesicles derived from adipose-derived stem cells (ADSC-EVs) promote wound by enhancing deposition.In this study, we explored whether ADSC-EVs could downregulate expression MMP-9 and improving deposition. potential effects on were tested both vitro vivo.We first evaluated effect...

To investigate the effect of circulating estrogen level on outcome free fat grafting in nude mice.Eighteen female mice aged 6-8 weeks (weighing, 20-25 g) were randomly divided into 3 groups ( n=6). The ovariectomized group treated with ovariectomy. high and normal only made same incision to enter peritoneum without given estradiol (0.2 mg/g) every days for 30 days. other two amount PBS At after operation, tail vein blood detected by ELISA kit, (0.3 mL) donated females was injected sub-scalp...

To discuss the possibility of constructing injectable tissue engineered adipose tissue, and to provide a new approach for repairing soft defects.

Dewanjee, M. K.; Wu, S.; Hsu, L. C.; Burke, G. W.; Twardock, A. R.; Gross, D. R. Author Information