A5102964475- Plant Pathogens and Fungal Diseases
- Liver Disease Diagnosis and Treatment
- Plant-Microbe Interactions and Immunity
- Fungal Plant Pathogen Control
- Bacterial Genetics and Biotechnology
- Bacterial biofilms and quorum sensing
- Legume Nitrogen Fixing Symbiosis
- Diet, Metabolism, and Disease
- Plant Pathogens and Resistance
- Entomopathogenic Microorganisms in Pest Control
- Mitochondrial Function and Pathology
- Metabolomics and Mass Spectrometry Studies
- Pharmacological Effects of Natural Compounds
- Bacteriophages and microbial interactions
- Mycorrhizal Fungi and Plant Interactions
- Microbial Metabolism and Applications
- Lipid metabolism and disorders
- Tuberous Sclerosis Complex Research
- Plant Micronutrient Interactions and Effects
- Microbial Natural Products and Biosynthesis
- Microbial Community Ecology and Physiology
- Hepatitis B Virus Studies
- Mesenchymal stem cell research
- RNA regulation and disease
- Neurogenesis and neuroplasticity mechanisms
Nanjing Forestry University
2017-2025
Heilongjiang Bayi Agricultural University
2018
Northeastern University
2017
South China Normal University
2008
Soybean root rot caused by the oomycete Phytophthora sojae is a serious soilborne disease threatening soybean production in China. Bacillus velezensis FZB42 model strain for Gram-positive plant growth-promoting rhizobacteria and able to produce multiple antibiotics. In this study, we demonstrated that B. can efficiently antagonize P. sojae. The underlying mechanism inhibition was then investigated. mutants deficient synthesis of lipopeptides (bacillomycin D fengycin), known have antifungal...
Cyclic diguanosine monophosphate (c-di-GMP) is a second messenger that plays crucial role in regulating biofilm development, yet the Gram-positive bacteria remains elusive. Here, we demonstrated dispersed cells from biofilms of Bacillus velezensis FZB42 exhibit unique phenotype and gene expression compared to planktonic cells. Transcriptomic analysis revealed 1327 downregulated 1298 upregulated genes, among which c-di-GMP phosphodiesterase coding yuxH was remarkably upregulated. Deletion led...
Bacteria switch between free-living and a multicellular state, known as biofilms, in response to cellular environmental cues. It is important understand how these cues influence biofilm development biofilms are not only ubiquitous nature but also causative agents of infectious diseases. often believed that any stress triggers formation means bacterial protection. In this study, we propose new mechanism for DNA damage may formation. We demonstrate Bacillus subtilis prevents cell...
Currently, almost all known regulators involved in bacterial phosphorus metabolism are proteins. In this study, we identified a conserved new small regulatory RNA (sRNA), named PhoS, encoded the 3' untranslated region (UTR) of phoPR genes Bacillus velezensis and B. subtilis. Expression phoS is strongly induced upon scarcity stimulated by transcription factor PhoP. Conversely, PhoS positively regulates PhoP translation binding to ribosome site (RBS) phoP mRNA. can promote biofilm formation...
Prodigiosin, a secondary metabolite extracted from Serratia marcescens (S. marcescens), could induce apoptosis in various cancer cells, with however low toxicity on normal cells. The red pigment was strain S. NJZT-1 isolated soil, which had antibacterial activity. Spectral analyses (LC-ESI-MS, UV-VIS spectrophotometry, infrared spectra and HPLC) TLC indicated the presence of prodigiosin extracellular bacterial culture extracts. effectively killed TSC2-null whose mutation resulted progressive...
Study results in this paper have indicated that the Holocene climate Xinjiang, Northwestern China has been alternating between wet and dry conditions, was punctuated with a series of abrupt shifts. A sediment core taken from Barkol Lake northern Xinjiang Northwest analyzed at 1cm interval for grain-size distribution. Abrupt shifts revealed by proxy occurred ca 1.4, 3.0, 4.3, 5.6, 8.0 cal kyr B.P., which were well correlated to both recorded North Atlantic Ocean (NAO) sea surface temperature...
This study is to investigate the association between hepatic expression of Yin Yang 1 (YY1) and progression non-alcoholic fatty liver disease (NAFLD) in patients undergoing bariatric surgery.Obese surgery were included. Liver tissues subjected quantitative real-time PCR, Western blot analysis, immunohistochemical assay, determine levels YY1.Totally 88 According NAFLD activity score (NAS), these divided into control (n = 12), steatosis 20), non-defining NASH 38), 18) groups. Significant...
Mycorrhizal helper bacteria (MHB) play an important role in mediating mycorrhizal symbiosis, which improves the growth and nutrient uptake of plants. This study examined growth-promoting effects mechanisms pine after inoculation with MHB Bacillus pumilus HR10 and/or Hymenochaete sp. Rl. The effect B. on Rl growth, enzyme activity gene expression related to mycorrhiza formation were determined. root activity, nitrogen, phosphorus, potassium content chlorophyll fluorescence Pinus thunbergii...
Bacillus velezensis FZB42 is a plant growth-promoting rhizobacterium (PGPR) and model microorganism for biofilm studies. Biofilms are required the colonization promotion of growth in rhizosphere. However, little known about how final stage life cycle regulated, when cells regain their motility escape mature to spread colonize new niches. In this study, non-annotated gene ccdC was found be involved process dispersion. We that ccdC-deficient strain maintained wrinkled state at late formation...
This study investigated the effect of Dendrobium officinale polysaccharide (DOP) on adipogenic differentiation rat bone marrow mesenchymal stem cells (BMSCs). DOP was extracted fresh officinale. Rat BMSCs were prepared, and then treated with 0 (control), 50, 100, 200, 400, 800 μg/mL DOP, respectively. The cell viability determined by MTT assay. quantitatively analyzed oil red O staining mRNA expressions related gene peroxisome proliferator-activated receptor gamma (PPARG), lipoprotein lipase...
This study is to investigate the effects of zoledronic acid (ZA) on TSC2-null cell proliferation and tumor progression recurrence in mouse models lymphangioleiomyomatosis (LAM).Subcutaneous LAM were established. Immunohistochemistry immunofluorescence performed detect protein expression levels. TUNEL assay was conducted apoptosis. Immunoprecipitation carried out determine interaction between proteins.ZA prevented growth cells both culture models. Compared with rapamycin, ZA more effectively...
Phosphorylation is crucial in regulating various biological processes. However, comprehensive phosphoproteomic profiling the termination of liver regeneration (LR) still missing. Here, we used Tandem Mass Tag (TMT) labeling coupled with phosphopeptide enrichment and two-dimensional (2D) liquid chromatography-mass spectrometry (LC-MS)/MS analysis to establish a global map mice at day 5 after partial hepatectomy (PH). Altogether, 9731 phosphosites from 3443 proteins were identified 7802 2980...
Abstract The prenylation of proteins is involved in a variety biological functions. However, it remains unknown whether plays an important role the morphogenesis cerebellum. To address this question, we generated mouse model, which geranylgeranyl pyrophosphate synthase ( Ggps1 ) gene inactivated neural progenitor cells developing We report that conditional knockout (cKO) leads to severe ataxia and deficient locomotion. identify underlying mechanisms, completed series cellular molecular...
Celtis sinensis is an adaptable species that widely grown in southern China. In June 2022, a leaf blotch disease of C. was observed Nanjing, Jiangsu, Based on morphological characterization, three isolates were identified as Alternaria species. Phylogenetic analysis combined ITS, GAPDH, TEF1-α, RPB2 and Alt 1 sequences the we obtained alternata koreana. Koch’s postulates fulfilled greenhouse, pathogenicity A. koreana determined by inoculation tests seedlings. The symptoms indoor consistent...
Celtis sinensis is a highly versatile species that commonly cultivated in the southern regions of China. In June 2022, leaf spot disease was detected C. Nanjing, Jiangsu, Based on morphological characteristics, three isolates were determined to be Alternaria species. A phylogenetic analysis combined ITS, GAPDH, TEF1-α, RPB2, and Alt 1 sequences identified we obtained as alternata A. koreana. We pathogenicity koreana leaves using inoculation through vivo experiments. Symptoms onset indoor...
S-ribosylhomocysteine lyase (LuxS) has been shown to regulate bacterial multicellular behaviors, typically biofilm formation. However, the mechanisms for regulation are still mysterious. We previously identified a malonylation modification on K124 and K130 of LuxS in plant growth-promoting rhizobacterium B. velezensis (FZB42). In this work, we investigated effects two sites formation other biological characteristics FZB42. The results showed that K124R mutation could severely impair...
Abstract Polycystic kidney disease (PKD) is a common hereditary disease. Although PKD occurrence associated with certain gene mutations, its onset regulatory mechanisms are still not well understood. Here, we first report that the key enzyme geranylgeranyl diphosphate synthase (GGPPS) specifically expressed in renal tubular epithelial cells of mouse kidneys. We aimed to explore role GGPPS PKD. In this study, established Ggpps fl/fl :Cdh16 cre model and compared phenotype wild‐type mice. A...
Abstract There are no known approved pharmacotherapies for non‐alcoholic fatty liver disease (NAFLD) in the clinical setting. Although studies have provided substantial evidence that geranylgeranyl diphosphate synthase (GGPPS) is a potential therapeutic target treatment of NAFLD corresponding drug screening rare. A GGPPS‐targeted inhibitor identified using structure‐based virtual small molecule method. The interaction 4‐AZ and GGPPS detected by microscale thermophoresis. degradation...
Phytophthora cinnamomi is a harmful microorganism that can infect Cinnamomum plants and cause the rotting of plant roots. It has been reported as infecting nearly 5000 types worldwide, such avocado trees, macadamia oak chestnut listed among 10 most destructive oomycetes 100 severely invasive species globally. A number chemical agents have applied in control phytophthora rot diseases because their easy application rapid effects. However, with extensive use agents, P. developed resistance....