Red blood cells (RBC) circulate the human body several hundred thousand times in their life span. Therefore, deformability is really important, especially when they pass through a local capillary whose diameter can be as narrow 3 μm. While there have been number of works discussing simulated such microchannel, far we examined literature, no work focusing on change shape after reciprocated mechanical stress has reported so far. One reasons that appropriate experimental systems to achieve...

ZigBee is a communication standard which considered to be suitable for wireless sensor networks. In ZigBee, device (with permanent 64-bit MAC address) said join network if it can successfully obtain 16-bit address from parent device. Parent devices calculate addresses their child by distributed assignment scheme. This easy implement, but restricts the number of children and depth network. We observe that policy too conservative, thus usually making utilization pool poor. Those cannot receive...

This paper proposes a new index for evaluating the stiffness-based deformability of cell using microchannel. In conventional approaches, transit time through microchannel is often utilized evaluation deformability. However, such includes both information stiffness and viscosity. this paper, we eliminate effect from viscosity, focus on only. We find that velocity varies when it enters channel, eventually reaches to equilibrium where becomes constant. The constant defined as cell, define...

This paper presents experimental investigations of passive mixing in a microfluidic channel with different zigzag angles. Zigzag is commonly used for because it does not need an additional control unit and can be easily implemented lab-on-a-chip system. In this work, channels six angles, from θ = 0° to 75°, are tested under ten flow rates corresponding Reynolds number 0.309 309. Two colored liquids mixed the performance evaluated based on color pixels region interest captured images....

Abstract Large deformability of erythrocytes in microvasculature is a prerequisite to realize smooth circulation. We develop novel tool for the three-step “Catch-Load-Launch” manipulation human erythrocyte based on an ultra-high speed position control by microfluidic “robotic pump”. Quantification shape recovery as function loading time uncovered critical window transition between fast and slow recoveries. The comparison with under depletion adenosine triphosphate revealed that cytoskeletal...

An on-chip deformability checker is proposed to improve the velocity–deformation correlation for red blood cell (RBC) evaluation. RBC has been found related human diseases, and can be evaluated based on velocity through a microfluidic constriction as in conventional approaches. The between transit amount of deformation provides statistical information deformability. However, such correlations are usually only moderate, or even weak, practical evaluations due limited range deformation. To...

The effect of hydrogen peroxide (H2O2) treatment on the microstructure and luminescent properties ZnO thin films has been investigated. Governed by high-resolution transmission electron microscopy selected-area diffraction patterns, oxygen radicals dissociated from H2O2 solution at room temperature substantially changed polycrystalline film into an insulator. In addition, photoluminescence spectra showed that had nearly no intensity ultraviolet emission, whereas it significantly enhanced...

A microfluidic design for evaluating red blood cell deformability with geometrical alignment mechanism is proposed.

Cell migration is crucial in physiological and pathological processes such as embryonic development wound healing; strongly guided by the surrounding nanostructured extracellular matrix. Previous studies have extensively studied cell on anisotropic nanotopographic surfaces; however, only a few reported isotropic surfaces. We herein, for first time, propose novel concept of adherable area using nanopore surfaces with sufficient depth adopting high aspect ratio. As pore size surface was...

A vision-based on-chip sensor for sensing local pressure inside a microfluidic device is proposed and evaluated in this paper. The determined from the change of color intensity chamber which pre-filled with colored fluid. working principle based on polydimethylsiloxane deformation. at point interest guided into deformation chamber, where structural stiffness softened by geometry, thus, deforms as result changes. Such transmitted to same-layer concentric chamber. causes fluid flowing or out...

OBJECTIVE: Various prediction scores have been developed to predict mortality in trauma patients, such as the shock index (SI), modified SI (mSI), age-adjusted (aSI), and (SI) multiplied by alert/verbal/painful/unresponsive (AVPU) score (SIAVPU). The SIAVPU is a novel scoring system but its accuracy for outcomes remains need of further validation. Therefore, we investigated four systems, including SI, mSI, aSI, SIAVPU, predicting mortality, admission intensive care unit (ICU), prolonged...

A rigid plate end-effector at the tip of a high-speed manipulator can remotely manipulate an object without grasping it. This paper discusses dynamic nonprehensile manipulation strategy to rotate thin deformable objects on with two degrees freedom (DOFs). The deformation due effects is exploited produce fast and stable rotation. By varying frequency rotational component plate's motion, we show that behavior mimics either sliding, walking, or running gait biped. We introduce model simulate...

An on-chip actuation transmitter for achieving fast and accurate cell manipulation is proposed. Instead of manipulating position by a directly connected macro-scale pump, polydimethylsiloxane deformation used as medium to transmit the generated from pump control position. This has three main advantages. First, dynamic response faster than conventional method with direct flow based on both theoretical modeling experimental results. The can be manipulated in simple harmonic motion up 130 Hz...

Fused deposition modeling (FDM) is the most commonly used 3D printing technique that directly extrudes molten materials layer by and overcomes constraints of fabricating complicate geometries. This fast, relatively easy to perform, thus more economical compared other methods. However, can be extruded FDM are limited polymers at present time. study aimed develop a fused for metals (FDMm) system deposit metallic structures on platform. An extruding nozzle designed researchers was connected...

Thin films of lithium manganese oxides were deposited by radio frequency magnetron sputtering. Negative electric bias was applied on the substrates during deposition. The exhibited a defected spinel structure, , as characterized X-ray diffraction and micro-Raman spectroscopy. As substrate increased, developed stronger (111) orientation. At high bias, tended to lose second phase oxide developed. Postanneal also carried out fabricate well-crystallized thin for comparison. film morphology...

Triple negative breast cancer (TNBC) is considered the most aggressive with high relapse rates and poor prognosis. Although great advances in development of therapy have been witnessed over past decade, treatment options for TNBC remain limited. In this study, we investigated effect potential underlying mechanism Hsp70 inhibitors, compound 1 6, on stem cells (BCSCs) cells. Our results showed that 6 exhibited potent tumor suppressive effects cell viability proliferation, effectively inhibited...

This study reports the preparation of CuInS2 (CIS) thin films on Mo-coated glass substrates using in situ sulfurization thermally evaporated Cu–In precursor a thermal evaporation chamber. The were at 1.2 × 10−6 Torr and further was carried out 8 10% H2S, balanced by Ar. alloy consist three types layered structures with fixed total thickness 800 nm. Different temperature–time profiles used during processes. Various phases identified both X-ray diffractometry (XRD) Raman spectroscopy, latter...

Photovoltaic CuInS2 (CIS) thin films were prepared from thermally evaporated Cu/In precursors, having various ratios, under different sulfurization conditions. The was carried out in a quartz tube furnace steady or flowing H2S/Ar gas mixture at temperatures ranging 450 to 550 °C. effects of the ratio and condition on grain size, surface morphology, roughness, microstructure defect formation resulting CIS examined. It found that depend processing Particularly, we show sub-bandgap generated...

An automatic system for evaluating single cell viscoelasticity is proposed and tested in this paper. The includes three main operations, they are the operations of catch, load launch. In catch operation, capable capturing a target by high-speed vision high-frequency flow control. captured pushed into narrow constriction operation. Different durations loading time applied to cells viscoelasticity. Finally, launched out from constriction, recovery response monitored characterization. Human red...

In this paper, we employ Fung's viscoelastic model discussed by Tiezzi and Kao to study the experimental data presented Sakamoto et al. for grasping objects using a parallel-jaw gripper. The contact modeling in paper is characterized two separate responses: elastic response temporal response. Two main intriguing results were found analysis of data. first consistency on normalized coefficients curve fitting during relaxation period grasping. Such suggests that proposed applicable task at...