A5080889493- Advanced NMR Techniques and Applications
- Advanced MRI Techniques and Applications
- Solid-state spectroscopy and crystallography
- Pediatric Urology and Nephrology Studies
- MRI in cancer diagnosis
- Electron Spin Resonance Studies
- Chronic Kidney Disease and Diabetes
- Atomic and Subatomic Physics Research
- Renal and Vascular Pathologies
- Diet and metabolism studies
Aarhus University
2020-2025
This study aims to investigate the effects of β-3-hydroxybutyrate (β-3-OHB) infusion on myocardial metabolic flexibility using hyperpolarized [2-13C]pyruvate magnetic resonance spectroscopy (MRS) in pig heart. We hypothesized that β-3-OHB will cause rapid, quantifiable alterations tricarboxylic acid (TCA) cycle flux as measured non-invasively by 13C MRS and reflect work. Five female Danish landrace pigs underwent during a hyperinsulinemic euglycemic clamp (HEC). Cardiac metabolism...
Acute ischemic stroke patients benefit from reperfusion in a short time-window after debut. Later treatment may be indicated if viable brain tissue is demonstrated and this outweighs the inherent risks of late reperfusion. Magnetic resonance imaging (MRI) with hyperpolarized [1-13C]pyruvate an emerging technology that directly images metabolism. Here, we investigated its potential to detect stroke. Stroke was induced pigs by intracerebral injection endothelin 1. During ischemia, rate...
Abstract Purpose Normothermic perfusion is an emerging strategy for donor organ preservation and therapy, incited by the high worldwide demand organs transplantation. Hyperpolarized MRI MRS using [1‐ 13 C]pyruvate other C‐labeled molecules pose a novel way to acquire highly detailed information about metabolism function in noninvasive manner. This study investigates use of this methodology as means monitor state ex vivo perfused porcine kidneys, context kidney graft research. Methods Kidneys...
Objectives Fibrosis is the final common pathway for chronic kidney disease and best predictor progression. Besides invasive biopsies, biomarkers its detection are lacking. To address this, we used hyperpolarized 13 C-pyruvate MRI to detect metabolic changes associated with fibrogenic activity of myofibroblasts. Materials Methods Hyperpolarized was performed in 2 pig models fibrosis (unilateral ureteral obstruction ischemia-reperfusion injury). The imaging data were correlated histology,...
Abstract Chronic kidney disease (CKD) is common and has huge implications for health mortality. It aggravated by intrarenal fibrosis, but the assessment of fibrosis limited to biopsies, which carry a risk complications sampling errors. This calls noninvasive modality diagnosing staging fibrosis. The current, exploratory study evaluates multiparametric MRI protocol including sodium imaging ( 23 Na‐MRI) determine opportunities within this assess injury as surrogate endpoint includes 43 pigs...
Purpose Increasing worldwide demand for cardiac transplantation has spurred new developments to increase the donor pool. Normothermic preservation of heart grafts is an emerging strategy improve utilization marginal grafts. Hyperpolarized MR using metabolic tracers such as [1‐ 13 C]pyruvate, provide a novel means investigating status without use ionizing radiation. We demonstrate this methodology examine ex vivo perfused porcine Methods Hearts from three 40‐kg Danish domestic pigs were...
Purpose The number of glomeruli is different in men and women, as they also present prevalence progression chronic kidney disease. A recent study has demonstrated a potential difference renal metabolism between sexes, explanation could be the differences number. This investigates correlation glomerular pyruvate healthy kidneys. Methods an experimental with rats ( N = 12). We used cationized‐ferritin MRI to visualize count hyperpolarized [1‐ 13 C]pyruvate map metabolism. Dynamic...