A5008354048- Immune cells in cancer
- Energy and Environment Impacts
- Air Quality and Health Impacts
- Cardiac Structural Anomalies and Repair
- Cardiac Fibrosis and Remodeling
- Garlic and Onion Studies
- Apelin-related biomedical research
- Atherosclerosis and Cardiovascular Diseases
- Adipokines, Inflammation, and Metabolic Diseases
- Cardiovascular Disease and Adiposity
- Pharmacology and Obesity Treatment
- Inflammation biomarkers and pathways
- Phagocytosis and Immune Regulation
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Lymphatic System and Diseases
- Inflammatory Biomarkers in Disease Prognosis
- Cholesterol and Lipid Metabolism
- Renin-Angiotensin System Studies
- Ferroptosis and cancer prognosis
- Vitamin D Research Studies
- Phytochemistry and biological activities of Ficus species
- Inflammasome and immune disorders
- Liver Disease Diagnosis and Treatment
- Mesenchymal stem cell research
- IL-33, ST2, and ILC Pathways
University of Pittsburgh
2022-2024
National Institute of Pharmaceutical Education and Research
2018-2024
University of Pittsburgh Medical Center
2024
BACKGROUND: Aldosterone has been described to initiate cardiovascular diseases by triggering exacerbated sterile vascular inflammation. The functions of CCL5 (C-C motif chemokine ligand 5) and its receptor CCR5 are well known in infectious diseases, their contributions aldosterone-induced injury hypertension remain unknown. METHODS: We analyzed the profile, blood pressure, renal damage wild-type (CCR5 +/+ ) knockout −/− mice treated with aldosterone (600 µg/kg per day for 14 days) while...
There is a large body of evidence that cellular metabolism governs inflammation, and inflammation contributes to the progression atherosclerosis. However, whether mitochondrial DNA synthesis affects macrophage function atherosclerosis pathology not fully understood. Here we show, by transcriptomic analyzes plaque macrophages, spatial single cell transcriptomics atherosclerotic plaques, functional experiments, (mtDNA) in macrophages are triggered vascular adhesion molecule 1 (VCAM-1) under...
Background Recently, our group identified increased platelet-mediated inflammation in type 2 diabetes (T2DM) patients, and it is a well-established risk factor for complications, particularly the development of cardiovascular diseases (CVD). Furthermore, vitamin D reported to play an important role modulation platelet hyperactivity immune function, although effect on not well studied. Hence, we aimed investigate supplementation T2DM patients. Methods After screening total 201 subjects,...
Peripheral ischemia caused by peripheral artery disease is associated with systemic inflammation, which may aggravate underlying comorbidities such as atherosclerosis and heart failure. However, the mechanisms of increased inflammation inflammatory cell production in patients remain poorly understood.
Type 2 diabetes mellitus (T2DM) is a well-established risk factor for the development of atherosclerotic coronary artery disease. Platelet hyperactivity and inflammation are associated with disease (CAD) in T2DM patients. We investigated status immune cells, platelet activation, platelet-immune cell interactions T2DM_CAD patients.The study population consisted four groups subjects, healthy control (CT, n = 20), (n 44), CAD 20) 38). immunome profiling were analysed by flow cytometry. The...
Ischemic heart disease and subsequent myocardial infarction (MI) is one of the leading causes mortality in United States around world. In order to explore pathophysiological changes after design future treatments, research models MI are required. Permanent ligation left coronary artery (LCA) mice a popular model investigate cardiac function ventricular remodeling post MI. Here we describe less invasive, reliable, reproducible surgical murine by permanent LCA. Our comprises an easily...
Diabetes mellitus (DM) is a chronic metabolic disorder associated with higher risk of having cardiovascular disease. Platelets play promising role in the pathogenesis complications diabetes. Since last several decades, garlic and its bioactive components are extensively studied diabetes complications. Our aim was to explore antiplatelet property allyl methyl sulfide (AMS) focusing on ameliorating platelet activation We used streptozotocin- (STZ-) induced diabetic rats as model for type 1...
Insulin resistance is a compromised response to insulin in target tissues such as liver. Emerging evidence shows that vascular endothelial cells (ECs) are critical mediating glucose metabolism. However, how liver ECs can regulate inflammation the setting of still unknown. Using genome-wide transcriptome analysis isolated from diabetic mice, we found enrichment genes involved epidermal growth factor receptor (Egfr) signaling. In line with this, hepatic sinusoidal mice had elevated levels Egfr...
Atherosclerotic cardiovascular disease is a leading cause of adult mortality worldwide. Macrophages play key role in atherogenesis by sustaining the local inflammatory response, facilitating accumulation immune cells atheromas that contribute to plaque development. Mitochondrial metabolism plays governing gene expression macrophages. Yet, mitochondrial biogenesis and DNA synthesis regulating macrophage inflammation not known. Vascular cell adhesion protein 1 (VCAM-1) expressed endothelial...
Ischemic heart disease and subsequent myocardial infarction (MI) is one of the leading causes mortality in United States around world. In order to explore pathophysiological changes after design future treatments, research models MI are required. Permanent ligation left coronary artery (LCA) mice a popular model investigate cardiac function ventricular remodeling post MI. Here we describe less invasive, reliable, reproducible surgical murine by permanent LCA. Our comprises an easily...
Air pollution has become a growing invisible killer in recent years and is major cause of morbidity mortality globally. India stands 10th among the highly polluted countries with an average PM10 level 134μg/m3 per year. It also reported that 99% India's population comes across air exceed World Health Organization Quality Guideline (AQG), PM2.5 permissible levels 10 μg/m3. Maternal exposure to serious health outcome offspring because it can affect embryonic phases development...
Air pollution has become a growing invisible killer in recent years and is major cause of morbidity mortality globally. India stands 10th among the highly polluted countries with an average PM10 level 134μg/m3 per year. It also reported that 99% India's population comes across air exceed World Health Organization Quality Guideline (AQG), PM2.5 permissible levels 10 μg/m3. Maternal exposure to serious health outcome offspring because it can affect embryonic phases development during gestation...
There is a large body of evidence that cellular metabolism governs inflammation. However, whether mitochondrial biogenesis alters macrophage function and disease pathogenesis not understood. We observed augmented DNA synthesis in macrophages human mouse atherosclerotic plaques. Unexpectedly, was triggered by vascular cell adhesion molecule 1 (VCAM-1) expressed this myeloid subset inflammatory conditions. RNA sequencing, bone marrow chimera, gene silencing overexpression experiments revealed...