A5061310462- Mycotoxins in Agriculture and Food
- Wheat and Barley Genetics and Pathology
- Carcinogens and Genotoxicity Assessment
- Plant Pathogens and Fungal Diseases
- Pesticide Residue Analysis and Safety
- Spectroscopy and Chemometric Analyses
- Dye analysis and toxicity
- Indoor Air Quality and Microbial Exposure
- Plant and fungal interactions
- Nuts composition and effects
- Fermentation and Sensory Analysis
- Listeria monocytogenes in Food Safety
- Plant Disease Resistance and Genetics
- Metabolomics and Mass Spectrometry Studies
- Agriculture, Plant Science, Crop Management
- Microbial Natural Products and Biosynthesis
- Plant Pathogens and Resistance
- Cholinesterase and Neurodegenerative Diseases
- Bacillus and Francisella bacterial research
- Natural Antidiabetic Agents Studies
- Genetics and Plant Breeding
- Synthesis and biological activity
- Phytochemistry Medicinal Plant Applications
- Pharmacology and Obesity Treatment
- Nanocomposite Films for Food Packaging
Institute of Sciences of Food Production
2016-2025
National Research Council
2013-2025
National Academies of Sciences, Engineering, and Medicine
2015
Human exposure assessment to deoxynivalenol (DON), aflatoxin B1 (AFB1), fumonisin (FB1), zearalenone (ZEA) and ochratoxin A (OTA) can be performed by measuring their urinary biomarkers. Suitable biomarkers of for these mycotoxins are DON + de-epoxydeoxynivalenol (DOM-1), M1 (AFM1), FB1, ZEA α-zearalenol (α-ZOL) β-zearalenol (β-ZOL) OTA, respectively. An UPLC-MS/MS multi-biomarker method was used detect measure incidence levels in urine samples 52 volunteers resident Apulia region Southern...
Food safety and quality awareness have reached significant relevance as consumers are more interested in authentic foods drinks with specific organoleptic values. Among foodstuffs, grape products can be contaminated by Ochratoxin A (OTA), a mycotoxin that occur red after infection Aspergillus carbonarius. The high affinity of pomace OTA makes its use advantageous an adsorbing/decontaminating material whether the is fresh, has undergone pressing, or stabilizing process. effects different...
Forty-five samples of a landrace sweet pepper (Capsicum annuum) widely cultivated in Basilicata (Italy) were screened for 17 mycotoxins and potential toxigenic fungal species. Two different LC-MS/MS methods used the determination aflatoxins, ochratoxin A (OTA), Fusarium zearalenone (ZEA), fumonisins (FB 1 FB 2 ), nivalenol (NIV), deoxynivalenol (DON), T-2 HT-2 toxins Alternaria altenuene (ALT), alternariol (AOH), monomethyl ether (AME), tentoxin (TTX) tenuazonic acid (TeA). Frequency species...
The worrying and constant increase in the quantities of food beverage industry by-products wastes is one main factors contributing to global environmental pollution. Since this a direct consequence continuous population growth, it imperative reduce waste production keep under control. Re-purposing agro-industrial wastes, giving them new life directions use, good first step direction, and, production, vegetables fruits account for significant percentage. In paper, brewery waste, cocoa bean...
The multi-biomarker approach was used to validate urinary biomarkers in piglets administered boluses contaminated with mixtures of deoxynivalenol (DON), aflatoxin B 1 (AFB ), fumonisin (FB zearalenone (ZEA) and ochratoxin A (OTA) at different concentrations. Boluses mycotoxins were prepared by slurrying freezedrying feed material fortified culture extracts selected toxigenic fungi. Piglets individually placed metabolic cages collect urine before gavage 24 h post dose. Urine samples...
Aspergillus carbonarius is the main responsible fungus of ochratoxin A (OTA) contamination grapes and derived products. To date, biosynthetic mechanism this mycotoxin has been partially elucidated. Availability genome sequence A. allowed identification a putative gene cluster involved in OTA biosynthesis. This region hosts previously characterized AcOTAnrps AcOTApks genes encoding two key enzymes pathway. At about 4,400 nucleotides downstream these loci, flavin dependent-halogenase came out...
Maize harvested in the Centane region of former Transkei, Eastern Cape Province, South Africa, by subsistence farmers has been shown over many seasons to be contaminated with fumonisin mycotoxins. However, there are limited data on presence other Two multimycotoxin LC-MS/MS methods were applied good and moldy maize samples, as separated themselves from 2011 harvest. One method involved extract cleanup multitoxin immunoaffinity columns before analysis for aflatoxins, fumonisins,...
Grape pomaces are increasingly being used as starting material in the industrial production of plant food supplements (PFS), coloring, and tartrates, but they at risk ochratoxin A (OTA) contamination, a mycotoxin with nephrotoxic carcinogenic effects. We analyzed 24 commercial PFS 13 coloring samples derived from Vitis vinifera, mainly pomaces, using HPLC-FLD method for OTA determination. was found 75% 69% levels <1.16-20.23 μg/kg <1.16-32.00 μg/kg, respectively. The four leavening agents...
Pancreatic lipase, a key enzyme for lipid absorption, is one of the most important targets treatment obesity, while natural compounds have recently attracted much interest as potential inhibitors this enzyme. Here, in an attempt to find new effective agents, methanolic extract from Moricandia arvensis (L.) DC. and its sub-extracts were investigated their inhibitory activity. The ability inhibit pancreatic lipase was verified through vitro evaluation prevention p-nitrophenyl caprylate...
The increasing pollution and wastage of food byproducts from agro-industrial production is an increasingly worrying issue. Grape one the most diffused fruit crops cultivated, grape pomace main solid byproduct obtained in winemaking process; interestingly, it rich health-beneficial bioactive molecules. In order to recover these molecules, this work, a green method has been developed, considering two pomaces different cultivars, namely, Petit Verdot Cabernet Sauvignon. extraction procedure, as...
Deoxynivalenol (DON) is an important mycotoxin produced by several species of Fusarium. It occurs often in wheat grain and frequently associated with significant levels its modified form DON-3-glucoside (DON-3-Glc). Ozone (O3) a powerful disinfectant oxidant, classified as GRAS (Generally Recognised As Safe), that reacts easily specific compounds including the mycotoxins aflatoxins, ochratoxin A, trichothecenes zearalenone. degrades DON aqueous solution can be effective for decontamination...
The efficacy of four agricultural byproducts (ABPs) and two commercial binders (CBs) to reduce the gastrointestinal absorption a mixture mycotoxins was tested in piglets using urinary mycotoxin biomarkers as indicator absorbed mycotoxins. Twenty-eight were administered bolus contaminated with containing or not ABP CB. Twenty-four hour urine collected analyzed for by multiantibody immunoaffinity-based LC-MS/MS method. Each contained 769 μg fumonisin B1 (FB1), 275 deoxynivalenol (DON), 29...
The determination of mycotoxin and metabolite concentrations in human animal urine is currently used for risk assessment intake measurement. In this study, pig (
A method for the determination of fumonisin B 1 (FB ) and 2 in different commercial maize-based products infants young children was developed tested a limited validation study involving 3 laboratories. The used extraction at 55 °C with an acidic mixture methanol-acetonitrile-phosphate/citrate buffer, clean-up through immunoaffinity column by high performance liquid chromatography automated pre-column derivatisation o -phthaldialdehyde. Recovery experiments were performed five spiking levels...
The performances of four LC-MS/MS methodologies for determination up to eight mycotoxin biomarkers in human urines were compared by involving three laboratories that analysed common urine samples spiked at two levels each biomarker. Each laboratory received a calibration solution, and the corresponding unspiked urine. spiking biomarker chosen considering naturally occurring limits quantification used participating laboratories. results evaluated their z- score values. percentage satisfactory...