A5019594363- Mitochondrial Function and Pathology
- Bacterial Genetics and Biotechnology
- ATP Synthase and ATPases Research
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Structure and Function
- Bacteriophages and microbial interactions
- Protein Structure and Dynamics
- Bacterial biofilms and quorum sensing
- RNA and protein synthesis mechanisms
- Pneumonia and Respiratory Infections
- Antibiotic Resistance in Bacteria
- Metabolism and Genetic Disorders
- Gene Regulatory Network Analysis
- Metabolomics and Mass Spectrometry Studies
- Adipose Tissue and Metabolism
- Biomedical Research and Pathophysiology
- Pancreatic function and diabetes
- Digestive system and related health
- Ubiquitin and proteasome pathways
University of California, Berkeley
2020-2025
The University of Texas Southwestern Medical Center
2025
Children's Medical Center
2025
Seoul National University
2013-2019
Significance Promoter recognition and specific transcription initiation depend on the binding of a sigma factor to core RNA polymerase in bacteria. The activity factors is often regulated through sequestration by cognate anti-sigma factors. Although Rsd known as for housekeeping σ 70 , which responsible most gene expression exponentially growing Escherichia coli cells, mechanism from during exponential phase growth has not been investigated previously. In this study, we demonstrate that...
Preferential sugar utilization is a widespread phenomenon in biological systems. Glucose usually the most preferred carbon source various organisms, especially bacteria where it taken up via phosphoenolpyruvate:sugar phosphotransferase system (PTS). The currently proposed model for glucose preference over non-PTS sugars enteric including E. coli strictly dependent on phosphorylation state of glucose-specific PTS component, enzyme IIAGlc (EIIAGlc). However, mechanism among largely unknown...
Abstract Carbon catabolite repression is a regulatory mechanism to ensure sequential utilization of carbohydrates and usually accomplished by genes for the transport metabolism less preferred carbon compounds more one. Although glucose mannitol share general components, enzyme I HPr, phosphoenolpyruvate-dependent phosphotransferase system (PTS) their transport, represses in manner dependent on operon repressor MtlR Escherichia coli . In recent study, we identified dephosphorylated form HPr...
Abstract Mitochondrial membrane potential (ΔΨm) is one of the key parameters controlling cellular bioenergetics. Investigation role ΔΨm in live cells complicated by a lack tools for its direct manipulation without off-target effects. Here, we adopted uncoupling protein UCP1 from brown adipocytes as genetically encoded tool ΔΨm. We validated ability exogenously expressed to induce uncoupled respiration and lower mammalian cells. expression lowered same extent chemical uncouplers but did not...
ABSTRACT Glycolysis is a conserved metabolic pathway that produces ATP and biosynthetic precursors. Here, we use mathematical modeling to investigate how the control of mammalian glycolytic enzymes through allostery mass action accomplishes various tasks homeostasis, such as controlling rate production, maintaining high stable levels, ensuring hydrolysis generates net excess energy. Our model uses data-derived enzyme equations, recapitulates key accurately predicts absolute concentrations...