A5002749329- CAR-T cell therapy research
- Fungal and yeast genetics research
- T-cell and B-cell Immunology
- Advancements in Semiconductor Devices and Circuit Design
- Genetics, Aging, and Longevity in Model Organisms
- Mycotoxins in Agriculture and Food
- Ubiquitin and proteasome pathways
- Vibrio bacteria research studies
- Silicon Carbide Semiconductor Technologies
- Viral Infectious Diseases and Gene Expression in Insects
- Insect and Pesticide Research
- Peptidase Inhibition and Analysis
- Fungal Biology and Applications
- Cholinesterase and Neurodegenerative Diseases
- Protein purification and stability
- Pesticide Exposure and Toxicity
- Protein Degradation and Inhibitors
- Polysaccharides and Plant Cell Walls
- Monoclonal and Polyclonal Antibodies Research
- Spaceflight effects on biology
- Bacterial Genetics and Biotechnology
- Nanowire Synthesis and Applications
- Plant-Microbe Interactions and Immunity
- Antifungal resistance and susceptibility
- CRISPR and Genetic Engineering
Stanford University
2023-2024
Cancer Institute (WIA)
2023
National Cancer Institute
2023
National Institutes of Health
2023
Center for Cancer Research
2023
University of Maryland, Baltimore County
2020-2022
Duke University
2017
Most current therapies that target plasma membrane receptors function by antagonizing ligand binding or enzymatic activities. However, typical mammalian proteins comprise multiple domains execute discrete but coordinated Thus, inhibition of one domain often incompletely suppresses the a protein. Indeed, targeted protein degradation technologies, including proteolysis-targeting chimeras1 (PROTACs), have highlighted clinically important advantages over inhibition2. generation...
A major limitation of chimeric antigen receptor (CAR) T cell therapies is the poor persistence these cells in vivo
daf-16/FoxO is required to survive starvation in Caenorhabditis elegans, but how daf-16IFoxO promotes resistance unclear. We show that restructures carbohydrate metabolism by driving carbon flux through the glyoxylate shunt and gluconeogenesis into synthesis of trehalose, a disaccharide glucose. Trehalose well-known stress protectant, capable preserving membrane organization protein structure during abiotic stress. Metabolomic, genetic, pharmacological analyses confirm increased trehalose...
Poor CAR T persistence limits cell therapies for B malignancies and solid tumors
The fungal cell-wall integrity signaling (CWIS) pathway regulates cellular response to environmental stress enable wall repair and resumption of normal growth. This complex, interconnected, has been only partially characterized in filamentous fungi. To better understand the dynamic perturbation, a β-glucan synthase inhibitor (micafungin) was added growing
Abstract Replication of Vibrio cholerae chromosome 2 (Chr2) initiates when the Chr1 locus, crtS (Chr2 replication triggering site) duplicates. The site binds Chr2 initiator, RctB, and binding increases is complexed with transcription factor, Lrp. How Lrp RctB how subsequently activated for initiation by crtS-Lrp complex remain unclear. Here we show that bends DNA possibly contacts acts commonly promote DNA-protein interactions. To understand enhances replication, isolated Tn-insertion point...
The ability to compartmentalize potentially lethal damage via septation appears provide filamentous fungi with a facile means tolerate diverse forms of stress. However, it remains unknown whether this mechanism is deployed in response all stress or limited specific perturbations.
Abstract The inhibition of acetylcholinesterase is regarded as the primary toxic mechanism action for chemical warfare agents. Recently, there have been numerous reports suggesting that metabolic processes could significantly contribute to toxicity. As such, we applied a multi-omics pipeline generate detailed cascade molecular events temporally occurring in guinea pigs exposed VX. Proteomic and metabolomic profiling resulted identification several enzymes precursors involved glycolysis TCA...
<h3>Background</h3> CAR T cell therapy is a promising therapeutic modality for cancer treatment, but poor persistence limits efficacy in patients.<sup>1</sup> cells with memory-like phenotype are associated durable patients and response to therapy,<sup>2</sup> thereby implicating memory as an important axis. Thus, strategies stably promote differentiation urgently needed. Here, we demonstrate that FOXO1 required the development of overexpression maintains gene expression programs enhances...
Abstract Background and Purpose Monoclonal antibodies (Ab) represent the fastest growing drug class. Knowledge of biophysical parameters ( k on , off K D ) that dictate Ab:receptor interaction is critical during discovery process. However, with increasing complexity Ab formats their targets, it became apparent existing technologies present limitations are not always suitable to determine these parameters. Therefore, novel affinity determination methods an unmet assay need. Experimental...