A5007849290- Fungal and yeast genetics research
- Fungal Biology and Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Antifungal resistance and susceptibility
- Fluid Dynamics and Mixing
- Microbial Natural Products and Biosynthesis
- Biofuel production and bioconversion
- Mycotoxins in Agriculture and Food
- Fermentation and Sensory Analysis
- Microbial Inactivation Methods
- Protein purification and stability
- Advanced Proteomics Techniques and Applications
- Polysaccharides and Plant Cell Walls
- Advanced Control Systems Optimization
- 3D Printing in Biomedical Research
- Cyclone Separators and Fluid Dynamics
Bristol-Myers Squibb (United States)
2021-2024
Bristol-Myers Squibb (Germany)
2023
University of Maryland, Baltimore County
2017-2020
The cyclic AMP (cAMP)-dependent protein kinase A (PKA) signaling pathway is well conserved across eukaryotes, and previous work has shown that it plays an important role in regulating development, growth, virulence a number of fungi. PKA activated response to extracellular nutrients acts regulate metabolism growth. While components the have been defined filamentous fungi, current understanding does not provide global perspective on function. Thus, this significant comprehensively identifies...
The fungal cell-wall integrity signaling (CWIS) pathway regulates cellular response to environmental stress enable wall repair and resumption of normal growth. This complex, interconnected, has been only partially characterized in filamentous fungi. To better understand the dynamic perturbation, a β-glucan synthase inhibitor (micafungin) was added growing
Fungal cell wall receptors relay messages about the state of to nucleus through Cell Wall Integrity Signaling (CWIS) pathway. The ultimate role CWIS pathway is coordinate repair damage and restore normal hyphal growth. Echinocandins such as micafungin represent a class antifungals that trigger by affecting synthesis β-glucans. To obtain better understanding dynamics response its multiple effects, we have coupled dynamic transcriptome analysis with morphological studies Aspergillus nidulans...
Fungal hyphal strength is an important phenotype which can have a profound impact on bioprocess behavior. Until now, there not efficient method allows its characterization. Currently available methods are very time consuming, thus, compromising their applicability in strain selection and process development. To overcome this issue, for fast easy, statistically verified quantification of relative tensile was developed. It involves off-line fragmentation high shear mixer followed by fragment...
In this article, we present four sets of data from high-throughput screening (HTS) studies different chemically defined media using an industrially relevant Chinese hamster ovary (CHO) cell line. While complex hydrolysate was used in the early phase process development and manufacturing a monoclonal antibody (mAb), here seek to determine appropriate for late development. Over 150 combinations basal media, feed supplements, such as polyphenolic flavonoid antioxidants (including rosmarinic...
Among many operating parameters that control cell culture environment, appropriate mixing and aeration are crucial for cells to meet oxygen demand in aerobic microbial mammalian production processes. A model-based manufacturing facility fit approach was applied define agitation air flow rates during process scale-up from laboratory manufacturing, of which computational fluid dynamics (CFD) the core modeling tool. The realizable k -ε turbulent dispersed Eulerian two-fluid model used simulate...
Abstract Fungal cell wall receptors relay messages about the state of to nucleus through Cell Wall Integrity Signaling (CWIS) pathway. The ultimate role CWIS pathway is coordinate repair damage and restore normal hyphal growth. Echinocandins such as micafungin represent a class antifungals that trigger by affecting synthesis β-glucans, filamentous fungi’s response these are fundamentally unknown. To obtain better understanding dynamics its multiple effects, we have coupled dynamic...