A5014819109- CAR-T cell therapy research
- Immune Cell Function and Interaction
- Allergic Rhinitis and Sensitization
- Asthma and respiratory diseases
- Lymphoma Diagnosis and Treatment
- Chronic Lymphocytic Leukemia Research
- Viral-associated cancers and disorders
- Hematopoietic Stem Cell Transplantation
- Cancer Immunotherapy and Biomarkers
- T-cell and B-cell Immunology
- Food Allergy and Anaphylaxis Research
- Virus-based gene therapy research
- Cytomegalovirus and herpesvirus research
- Immunotherapy and Immune Responses
- Dermatology and Skin Diseases
- Pregnancy and preeclampsia studies
- Pregnancy and Medication Impact
- Acute Lymphoblastic Leukemia research
- Health Systems, Economic Evaluations, Quality of Life
- Pharmaceutical Economics and Policy
- Respiratory and Cough-Related Research
- Chronic Myeloid Leukemia Treatments
- Eosinophilic Esophagitis
- Biosimilars and Bioanalytical Methods
- T-cell and Retrovirus Studies
Bristol-Myers Squibb (United States)
2018-2024
Italian Medicines Agency
2021-2024
Humboldt-Universität zu Berlin
2021-2022
Charité - Universitätsmedizin Berlin
2014-2022
Istituti di Ricovero e Cura a Carattere Scientifico
2006-2022
Freie Universität Berlin
2021-2022
Istituto Superiore di Sanità
2022
Ministero dell'Economia e delle Finanze
2022
Mario Negri Institute for Pharmacological Research
2022
Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico
2022
Transplantation of hematopoietic stem cells from partially matched family donors is a promising therapy for patients who have hematologic cancer and are at high risk relapse. The donor T-cell infusions associated with such transplantation can promote post-transplantation immune reconstitution control residual disease.
Severe and fatal viral infections remain common after hematopoietic stem cell transplantation. Adoptive transfer of cytotoxic T lymphocytes (CTLs) specific for Epstein–Barr virus (EBV), cytomegalovirus (CMV), adenoviral antigens can treat that are impervious to conventional therapies, but broader implementation extension additional viruses is limited by competition between virus-derived time-consuming laborious manufacturing procedures. We now describe a system rapidly generates single...
Ablative treatments such as photothermal therapy (PTT) are attractive anticancer strategies because they debulk accessible tumor sites while simultaneously priming antitumor immune responses. However, the response following thermal ablation is often insufficient to treat metastatic disease. Here we demonstrate that PTT induces expression of proinflammatory cytokines and chemokines promotes maturation dendritic cells within tumor-draining lymph nodes, thereby T cell Unexpectedly, however,...
The antitumor activity of chimeric antigen receptor (CAR)-redirected CTLs should be enhanced if it were possible to increase their proliferation and function after adoptive transfer without concomitantly increasing the regulatory T cells (Treg). Here, we explored whether lack IL-7Rα in Treg can exploited by targeted manipulation interleukin-7 (IL-7) cytokine-cytokine axis CAR-engrafted Epstein-Barr Virus-specific (EBV-CTLs) selectively augment growth even presence Treg.We generated a...
Pollen-food syndrome (PFS) is heterogeneous with regard to triggers, severity, natural history, comorbidities, and response treatment. Our study aimed classify different endotypes of PFS based on IgE sensitization panallergens.We examined 1271 Italian children (age 4-18 years) seasonal allergic rhinoconjunctivitis (SAR). Foods triggering were acquired by questionnaire. Skin prick tests performed commercial pollen extracts. panallergens Phl p 12 (profilin), Bet v 1 (PR-10), Pru 3 (nsLTP)...
The integration characteristics of retroviral (RV) vectors increase the probability interfering with regulation cellular genes, and account for a tangible risk insertional mutagenesis in treated patients. To assess potential genotoxic conventional or self-inactivating (SIN) γ-RV lentiviral (LV) independently from biological consequences insertion event, we developed quantitative assay based on real-time reverse transcriptase—PCR low-density arrays to evaluate alterations gene expression...
Abstract Purpose: Systemic administration of recombinant interleukin (IL)-2 is used to support the expansion and persistence adoptively transferred antigen-specific CTLs in patients with cancer. However, IL-2 also expands regulatory T cells (Treg) that turn impair antitumor activity CTLs. As IL-15 approaching clinical applications, we assessed effects this cytokine on proliferation presence Tregs. We model adoptive transfer Epstein–Barr virus (EBV)-CTLs, as these induce responses...
Summary Background Adherence to controller therapy in allergic diseases is low. Telemonitoring has been proposed improve adherence treatment chronic diseases. However, this strategy never tested rhinoconjunctivitis. Objective To test whether Internet‐based telemonitoring during the grass‐pollen season of children with rhinoconjunctivitis may enhance treatment. Methods Children and adolescents, 5–18 years old, moderate‐to‐severe seasonal grass pollen requiring daily administration nasal...
Abstract Background In early childhood, the allergen‐specific IgG repertoire is mainly directed to animal and vegetable food molecules infrequently airborne molecules. It unknown whether this pattern maintained throughout childhood. Objective To investigate evolution of IgE responses a broad panel allergenic from birth age 10 years. Methods We examined sera collected between years participants in German Multicentre Allergy Study, cohort born 1990. The (cutoff ≥0.30 ISU ) ≥0.10 35 genuine...
Grass pollen-related seasonal allergic rhinoconjunctivitis (SARg) is clinically heterogeneous in severity, comorbidities, and response to treatment. The component-resolved diagnostics disclosed also a high heterogeneity at molecular level. Our study aimed analyzing the characteristics of IgE sensitization Phleum pratense molecules investigating diagnostic relevance such childhood.We examined 1120 children (age 4-18 years) with SARg. Standardized questionnaires on atopy were acquired through...
TPS465 Background: Treatment options for PDAC are limited; thus, new therapies that can improve outcomes and extend survival needed. is associated with high infiltration by tumor-associated macrophages (TAMs) inhibit antitumor T-cell activity. Blocking colony-stimulating factor 1 receptor (CSF-1R) signaling—which supports the recruitment, differentiation, maintenance of immunosupressive in tumors—may lead to depletion TAMs upregulation checkpoints. Cabira, a humanized IgG4 monoclonal...
Background Complete diagnosis and therapy of seasonal allergic rhinoconjunctivitis require evidence that exposure to the sensitizing pollen triggers symptoms. Electronic clinical diaries, by recording disease severity scores exposure, can demonstrate this association. However, patients who spontaneously download an e-diary app show very low adherence their recording. Objective The objective our study was assess with rhinitis symptom via explicitly prescribed allergist within a blended care...
Summary Background The identification of the primary sensitizing pollen is difficult in Southern European patients with Seasonal Allergic Rhinitis (SAR) if sensitized to various sources overlapping seasonality. A more precise diagnosis obtained by IgE assays allergen molecules, currently available as singleplex or microarrays. Objectives To test analytical performance a multi‐parameter immunoblot molecular “Pollen Test” specifically designed antibodies extracts and molecules clinically...
Many different symptom (medication) scores are nowadays used as measures of allergic rhinoconjunctivitis severity in individual patients and clinical trials. Their differences contribute to the heterogeneity primary end-point meta-analyses, so that calls for score harmonization have been launched.To prospectively compare six rhinitis (AR) against pollen counts at both population levels.Two groups children with seasonal AR grass sensitization were recruited Ascoli, Italy (n = 76) Berlin,...
Pollen-related seasonal allergic rhinoconjunctivitis (SAR) is a very frequent pediatric disease in Westernized countries. Risk factors and phenotypes have been thoroughly examined several cross-sectional studies. By contrast, only few studies evolution patient cohorts. We investigated predictive biomarkers of large cohort children with SAR.During 2015-2017 (follow-up), we re-examined 401 patients from those enrolled 2009-2011 (baseline) by the "Panallergens Pediatrics" study, multicenter...
Previous studies of serum total IgE (t-IgE) were not able to discriminate well-enough atopic from non-atopic subjects, that is, with or without serum-specific antibodies allergens.To model growth curves the levels in children sensitization (hereafter defined as "normal" t-IgE levels) and test their usefulness predicting sensitization.The German Multicentre Allergy Study (MAS), a birth cohort 1314 recruited newborns, began 1990 examined participants until age 20 years. Total specific (t-IgE,...
Abstract Background Allergen immunotherapy (AIT) is the only disease‐modifying treatment in patients with seasonal allergic rhinoconjunctivitis (SAR). Its efficacy depends on precise identification of triggering allergen. However, diagnostics based retrospective clinical history and sensitization to whole extracts (SWE) often leads equivocal results. Objectives To assess usability impact a recently established algorithm for decision support system (@IT2020‐CDSS) SAR its diagnostic steps...
The low frequency of naturally occurring regulatory T cells (nTregs) in peripheral blood and the suboptimal protocols available for their ex vivo expansion limit development clinical trials based on adoptive transfer these cells. We have, therefore, generated a simplified, robust cost-effective platform large-scale nTregs using gas permeable static culture flask (G-Rex) compliance with Good Manufacturing Practice. More than 109 putative Tregs co-expressing CD25 CD4 molecules (92±5%) FoxP3...
<h3>Background</h3> Virus-specific T-cells (VSTs) proliferate exponentially after adoptive transfer into hematopoietic stem cell transplant (HSCT) recipients, eliminate virus infections, then persist and provide long-term protection from viral disease. If VSTs behaved similarly when modified with tumor-specific chimeric antigen receptors (CARs), they should have potent anti-tumor activity. This theory was evaluated by Cruz et al. in a previous clinical trial CD19.CAR-modified VSTs, but there...