A5012151234- Cellular Mechanics and Interactions
- TGF-β signaling in diseases
- Cancer Cells and Metastasis
- RNA Research and Splicing
- Cell Adhesion Molecules Research
- Caveolin-1 and cellular processes
- Sarcoma Diagnosis and Treatment
- Gastric Cancer Management and Outcomes
- Oxidative Organic Chemistry Reactions
- Helicobacter pylori-related gastroenterology studies
- Uterine Myomas and Treatments
- Heat shock proteins research
- Chemical Synthesis and Reactions
- Muscle Physiology and Disorders
- Cytokine Signaling Pathways and Interactions
- Signaling Pathways in Disease
- Growth Hormone and Insulin-like Growth Factors
- Inorganic and Organometallic Chemistry
- Photochromic and Fluorescence Chemistry
- Viral Infectious Diseases and Gene Expression in Insects
- Organophosphorus compounds synthesis
- Hippo pathway signaling and YAP/TAZ
- Estrogen and related hormone effects
- Genetic factors in colorectal cancer
- Microtubule and mitosis dynamics
Wakayama Medical University
2021-2024
Tokushima University
2024
Osaka University
2004-2023
Tokyo Medical and Dental University
2015
Hyogo Medical University
2010-2012
Kyoto Prefectural University of Medicine
2010
Hyogo University
2010
Tohoku University
2002-2004
The University of Kitakyushu
1994
Osaka Research Institute of Industrial Science and Technology
1980-1981
Epithelial–mesenchymal transition (EMT) is a critical process occurring during embryonic development and in fibrosis tumor progression. Dissociation of cell–cell contacts remodeling the actin cytoskeleton are major events EMT. Here, we show that myocardin-related transcription factors (MRTFs; also known as MAL MKL) mediators transforming growth factor β (TGF-β) 1–induced In all epithelial cell lines examined here, TGF-β1 triggers nuclear translocation MRTFs. Ectopic expression...
Epithelial–msenchymal transition (EMT) is closely associated with cancer and tissue fibrosis. The nuclear accumulation of myocardin-related transcription factor A (MRTF-A/MAL/MKL1) plays a vital role in EMT. In various cells treated CCG-1423, novel inhibitor Rho signaling, the MRTF-A inhibited. However, molecular target this has not yet been identified. study, we investigated mechanism effect CCG-1423. interaction between importin α/β1 was inhibited by but monomeric G-actin binding to We...
The podosome and invadopodium are dynamic cell‐adhesion structures that degrade the extracellular matrix (ECM) promote cell invasion. We recently reported actin‐binding protein caldesmon is a pivotal regulator of formation. Here, we analyzed caldesmon's involvement in podosome/invadopodium‐mediated invasion by transformed cancer cells. ectopic expression reduced number podosomes/invadopodia decreased ECM degradation activity, resulting suppression Conversely, depletion facilitated formation...
Insulin-like growth factor-I (IGF-I) plays a role in mutually exclusive processes such as proliferation and differentiation variety of cell types. IGF-I is potent mitogen motogen for dedifferentiated vascular smooth muscle cells (VSMCs) vivo vitro. However, differentiated VSMCs, only required maintaining the phenotype. Here we investigated VSMC phenotype-dependent signaling biological triggered by IGF-I. In activated protein-tyrosine phosphatase, SHP-2, recruited insulin receptor substrate-1...
Glucocorticoids (GCs) play important roles in numerous cellular processes, including growth, development, homeostasis, inhibition of inflammation, and immunosuppression. Here we found that GC-treated human lung carcinoma A549 cells exhibited the enhanced formation thick stress fibers focal adhesions, resulting suppression cell migration. In a screen for GC-responsive genes encoding actin-interacting proteins, identified caldesmon (CaD), which is specifically up-regulated response to GCs. CaD...
Podosomes are dynamic cell adhesion structures that degrade the extracellular matrix, permitting matrix remodeling. Accumulating evidence suggests actin and its associated proteins play a crucial role in podosome dynamics. Caldesmon is localized to podosomes, expression down-regulated transformed cancer cells. Here we studied regulatory mode of caldesmon formation Rous sarcoma virus-transformed fibroblasts. Exogenous analyses revealed represses triggered by N-WASP-Arp2/3 pathway. Conversely,...
Abstract Background and Aims: Proton pump inhibitors (PPIs) are generally used to prevent delayed bleeding after endoscopic submucosal dissection (ESD) heal the artificial ulcers. However, it remains controversial whether PPIs or histamine‐2 receptor antagonists (H 2 RAs) more effective in preventing ESD. We prospectively compared effects of omeprazole famotidine promoting ulcer healing Methods: A total 158 patients (155 early gastric cancers three adenomas) were randomly assigned PPI group...
Abstract Although enhanced thymosin β4 (TMSB4X/Tβ4) expression is associated with tumor progression and metastasis, its tumor-promoting functions remain largely unknown. Here, it demonstrated that TGFβ facilitates Tβ4 leads to the activation of myocardin-related transcription factors (MRTF), which are coactivators serum response factor (SRF) regulate genes critical for epithelial–mesenchymal transition (EMT) metastasis. In murine mammary gland cells (NMuMG), upregulation required full...
Myocardin is an important transcriptional regulator in smooth and cardiac muscle development. We noticed that the expression of myocardin was markedly downregulated human uterine leiomyosarcoma cells. Restoration induced reexpression marker proteins formation well-developed actin fibers. A concomitant increase a cyclin-dependent kinase inhibitor, p21, led to significantly reduced cell proliferation, via p21's inhibition G(1)-S transition. p21 promoter-reporter assay showed increased promoter...
Glucocorticoids (GCs) mediate the effects of stress to cause structural plasticity in brain regions such as hippocampus, including simplification dendrites and shrinkage dendritic spines. However, molecular mechanics linking GCs these remain largely unclear. Here, we demonstrated that corticosterone (CORT) reduces expression levels caldesmon (CaD), causing spines become vulnerable. CaD regulates cell motility by modulating actin-myosin system actin filament stability. In cultured rat...
Abstract The roles of myocardin-related transcription factor A (MRTF-A) and MRTF-B in vascular endothelial cells are not completely understood. Here, we found a novel regulatory mechanism for MRTF-A/B function. tend to accumulate the nucleus arterial vivo human aortic (HAoECs) vitro . In HAoECs, nuclear localization was significantly affected by Y27632 or latrunculin B, primarily due reduced binding G-actin part, low level MRTF-A phosphorylation ERK. downregulation serum depletion...
Autophagy of glycogen (glycophagy) is crucial for the maintenance cellular glucose homeostasis and physiology in mammals. STBD1 can serve as an autophagy receptor to mediate glycophagy by specifically recognizing relevant key autophagic factors, but with poorly understood mechanisms. Here, we systematically characterize interactions related saccharides, determine crystal structure CBM20 domain maltotetraose, uncovering a unique binding mode involving two different oligosaccharide-binding...
Myocardin (Myocd) and Myocd-related transcription factors (MRTFs) are robust coactivators of serum response factor (SRF). RPEL motifs monomeric globular actin (G-actin) binding elements that regulate MRTF localization activity. However, the function motif in Myocd is largely unknown because its low affinity for G-actin. Here, we demonstrated bound to actin-related protein 5 (Arp5) instead conventional actin, resulting a significant suppression In addition, Arp5 DNA domain SRF via C-terminal...
CCG-1423 suppresses several pathological processes including cancer cell migration, tissue fibrosis, and the development of atherosclerotic lesions. These suppressions are caused by inhibition myocardin-related transcription factor A (MRTF-A), which is a critical for epithelial–mesenchymal transition (EMT). can therefore be potent inhibitor EMT. related compounds, CCG-100602 CCG-203971 possess similar biological activities. Although these compounds comprised two stereoisomers, differences in...
Inflammatory response induces phenotypic modulation of fibroblasts into myofibroblasts. Although transforming growth factor-βs (TGF-βs) evoke such transition, the details mechanism are still unknown. Here, we report that a LIM domain protein, cysteine-and glycine-rich protein 2 (CSRP2 [CRP2]) plays vital role in functional expression profile myofibroblasts and cancer-associated (CAFs). Knock-down CRP2 severely inhibits smooth muscle cell (SMC) genes, motility, CAF-mediated collective...
Two members of the myocardin protein family, myocardin-related transcription factor (MRTF)-A and MRTF-B are co-activators serum response (SRF). We recently reported that MRTF-A/B activates several actin cytoskeletal/focal adhesion genes SRF dependently, thereby enhancing formation stress fibers focal adhesions. Here, we showed levels caldesmon tropomyosin, both SRF/MRTF-regulated cytoskeletal proteins, were reduced in rat intestinal epithelial (RIE) cell lines had been transformed with...
Dd-TRAP1 is a Dictyostelium homologue of tumor necrosis factor receptor-associated protein 1 (TRAP-1). located in the cortex cells growing at low density, but was found to be translocated mitochondria with help novel prestarvation that accumulated growth medium along increased cell densities. The knockdown mutant (TRAP1-RNAi cells) exhibited significant defect response. Although TRAP1-RNAi showed normal expressions classical genes [dscA (discoidin I) and car1 (carA; cAMP receptor)],...
Myogenic regulatory factors (MRFs) are pivotal transcription in myogenic differentiation. MyoD commits cells to the skeletal muscle lineage by inducing genes through recruitment of chromatin remodelers its target loci. This study showed that actin-related protein 5 (Arp5) acts as an inhibitory regulator and MyoG binding their cysteine-rich (CR) region, which overlaps with region essential for epigenetic functions. Arp5 expression was faint tissues. Excessive mouse hind limbs caused fiber...
Compounds classified as benzoylphenylurea (BPU), such diflubenzuron (DFB), are used insecticides. Although BPU disrupts molting by inhibiting chitin biosynthesis and exhibits insecticidal activity, their exact mode of action remains unknown. Since epidermal cells proliferate morphologically change from squamous to columnar during the early stages insect molting, we speculate that a transition similar epithelium mesenchyme occurs may inhibit this transition. Here, addressed possibility. We...