A5065724244- Immune Cell Function and Interaction
- Toxoplasma gondii Research Studies
- T-cell and B-cell Immunology
- Herpesvirus Infections and Treatments
- Parasitic Infections and Diagnostics
- Immune Response and Inflammation
- Bladder and Urothelial Cancer Treatments
- FOXO transcription factor regulation
- Adrenal Hormones and Disorders
- Genomics, phytochemicals, and oxidative stress
- Immunotherapy and Immune Responses
- Antimicrobial Peptides and Activities
- Legal Issues in South Africa
- Myasthenia Gravis and Thymoma
- Bacterial biofilms and quorum sensing
- Cancer, Stress, Anesthesia, and Immune Response
- RNA regulation and disease
- SARS-CoV-2 and COVID-19 Research
- Reproductive System and Pregnancy
- Ferroptosis and cancer prognosis
- Long-Term Effects of COVID-19
- Cytomegalovirus and herpesvirus research
- T-cell and Retrovirus Studies
- CAR-T cell therapy research
- Corporate Insolvency and Governance
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2015-2025
Universidade do Porto
2013-2024
Instituto de Biologia Molecular e Celular
2013-2022
University of Minho
2013
The activation of TLRs by microbial molecules triggers intracellular-signaling cascades and the expression cytokines such as IL-10. Il10 is tightly controlled to ensure effective immune responses, while preventing pathology. Maximal TLR-induction transcription in macrophages requires signaling through MAPKs, ERK, p38. Signals via p38 downstream TLR4 also regulate IL-10 at post-transcriptional level, but whether this mechanism operates other not clear. We compared regulation production TLR2...
Within the thymus, thymic epithelial cells (TECs) provide dedicated stroma microenvironments for T cell development. Because TEC functionality is sensitive to aging and cytoablative therapies, unraveling molecular elements that coordinate their thymopoietic role has fundamental clinical implications. Particularly, selection of CD4 depends on interactions between TCRs expressed precursors self-peptides:MHC II complexes presented by cortical TECs (cTECs). Although...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has evolved to variants associated with milder disease. We employed the k18-hACE2 mouse model study how differences in course of infection by SARS-CoV-2 alpha, delta, and omicron relate tissue pathology immune response triggered. documented a variant-specific pattern severity, inducing discrete lung blood responses differentially impacting primary lymphoid organs. Infections alpha delta promoted bone marrow (BM) emergency...
Within the thymus, thymic epithelial cells (TECs) create dedicated microenvironments for T cell development and selection. Considering that TECs are sensitive to distinct pathophysiological conditions, uncovering molecular elements coordinate their thymopoietic role has important fundamental clinical implications. Particularly, medullary (mTECs) play a crucial in central tolerance. Our previous studies, along with others, suggest mTECs depend on factors linked genome-protecting pathways, but...
In view of the growing industrial use Bacterial cellulose (BC), and taking into account that it might become airborne be inhaled after processing, assessing its potential pulmonary toxic effects assumes high relevance. this work, murine model was used to assess exposure respirable BC nanofibrils (nBC), obtained by disintegration produced
Autoimmune regulator+ (Aire) medullary thymic epithelial cells (mTECs) play a critical role in tolerance induction. Several studies demonstrated that Aire+ mTECs differentiate further into Post-Aire cells. Yet, the identification of terminal stages mTEC maturation depends on unique fate-mapping mouse models. Herein, we resolve this limitation by segmenting mTEChi (MHCIIhi CD80hi ) compartment mTECA/hi (CD24- Sca1- ), mTECB/hi (CD24+ and mTECC/hi Sca1+ ). While included mostly Aire-expressing...
Most patients with muscle-invasive bladder cancer (MIBC) are not cured platinum chemotherapy. Up-regulation of nuclear factor kappa light-chain enhancer activated B cells (NF-κB) is a major mechanism underlying chemoresistance, suggesting that its pharmacological inhibition may increase efficacy. NF-κB signaling was investigated in two patient cohorts. The Cancer Genome Atlas (TCGA) used to correlate and survival. efficacy cisplatin plus the inhibitor dimethylaminoparthenolide (DMAPT) versus...
It is well established that CD8(+) T cells play an important role in protective immunity against protozoan infections. However, their the course of Neospora caninum infection has not been fully elucidated. Here we report CD8-deficient mice infected with N. presented higher parasitic loads brain and lungs lower spleen immunity-related GTPases than wild-type counterparts. Moreover, adoptive transfer splenic sorted from caninum-primed immunosufficient C57BL/10 ScSn prolonged survival...
Summary The adipose tissue can make important contributions to immune function. Nevertheless, only a limited number of reports have investigated in lean hosts the response elicited this upon infection. Previous studies suggested that intracellular protozoan Neospora caninum might affect physiology. Therefore, we mice challenged with if cell populations within different anatomical locations could be differently affected. Early infection, parasites were detected and by 7 days infection...
Abstract The murine model has been widely used to study the host immune response Neospora caninum . However, in most studies, intraperitoneal route was preferentially establish infection. Here, C57BL/6 mice were infected with N. tachyzoites by intragastric route, as it more closely resembles natural of infection through gastrointestinal tract. elicited T-cell mediated evaluated intestinal epithelium and mesenteric lymph nodes (MLN). Early upon parasitic challenge, IL-12 production...
Summary N eospora caninum is an A picomplexa parasite that in the last two decades was acknowledged as main pathogenic agent responsible for economic losses cattle industry. In present study, effectiveness of intranasal immunization with . membrane antigens plus C p G adjuvant assessed a murine model intragastrically established neosporosis. Immunized mice presented lower parasitic burden brain on infection 5 × 10 7 tachyzoites, showing significant protection achieved by this strategy....
Abstract Here we report that lean mice infected with the intracellular parasite Neospora caninum show a fast but sustained increase in frequency of IFN-γ-producing cells noticeable distinct adipose tissue depots. Moreover, IFN-γ-mediated immune memory could be evoked vitro antigen-stimulated stromal vascular fraction collected from one year before. Innate or innate-like such as NK, NK T and TCRγδ + cells, also CD4 CD8 TCRβ lymphocytes contributed to IFN-γ production observed since day...
The thymus stroma constitutes a fundamental microenvironment for T-cell generation. Despite the chief contribution of thymic epithelial cells, recent studies emphasize regulatory role mesenchymal cells in function. Mesenchymal progenitors are suggested to exist postnatal thymus; nonetheless, an understanding their nature and mechanism controlling homeostasis vivo remains elusive. We resolved two new fibroblast subsets with distinct developmental features. Whereas CD140αβ+GP38+SCA-1-...
Poly-N-acetylglucosamine (PNAG) is a major component of the Staphylococcus epidermidis biofilm extracellular matrix. However, it not yet clear how this polysaccharide impacts host immune response and infection-associated pathology. Faster neutrophil recruitment bacterial clearance were observed in mice challenged intraperitoneally with S. cells PNAG-producing 9142 strain than similarly isogenic PNAG-defective M10 mutant. Moreover, intraperitoneal priming exacerbated liver inflammatory...
<title>Abstract</title> Within the thymus, thymic epithelial cells (TECs) create dedicated microenvironments for T-cell development and selection. Considering that TECs are sensitive to distinct pathophysiological conditions, uncovering molecular elements coordinate their thymopoietic role has important fundamental clinical implications. Particularly, medullary (mTECs) play a crucial in central tolerance. Our previous studies, along with others, suggest mTECs depend on factors linked...