A5040148752- Actinomycetales infections and treatment
- Infectious Diseases and Mycology
- Mycobacterium research and diagnosis
- CRISPR and Genetic Engineering
- Refrigeration and Air Conditioning Technologies
- Diphtheria, Corynebacterium, and Tetanus
- Building Energy and Comfort Optimization
- Advanced biosensing and bioanalysis techniques
- Infectious Disease Case Reports and Treatments
- Adsorption and Cooling Systems
- Heat Transfer and Boiling Studies
- Heat Transfer and Optimization
- Genomics and Phylogenetic Studies
- Flow Measurement and Analysis
- Phytoplasmas and Hemiptera pathogens
- Biosensors and Analytical Detection
- Fusion materials and technologies
- Wireless Communication Networks Research
- Zoonotic diseases and public health
- Solar-Powered Water Purification Methods
- Wireless Networks and Protocols
- Hydraulic and Pneumatic Systems
- Bacterial Identification and Susceptibility Testing
- COVID-19 epidemiological studies
- Advanced Thermodynamic Systems and Engines
National Institute for Communicable Disease Control and Prevention
2020-2024
China Academy of Building Research
2022-2024
State Key Laboratory of Building Safety and Built Environment
2022-2024
Shandong First Medical University
2024
Shandong Provincial Hospital
2024
Center for Disease Control
2023
Tibet University
2020
Nocardia cyriacigeorgica has gradually become a common pathogen in clinical microbial infections. Identification of at the species level is essential to assess susceptibility and pathogenicity antimicrobials. However, there no suitable method for rapid accurate laboratory detection N. . In this study, we combined PCR amplification with CRISPR-Cas12a system establish novel platform, named CRISPR-PCR, applied it samples. The Cas12a protein exhibited collateral cleavage activity following...
Pseudomonas aeruginosa is a major bacterial pathogen causing nosocomial infections and accounts for morbidity mortality among patients with cystic fibrosis. An accurate, sensitive, rapid method to detect P. critical the early control of infection patient management. In this study, we established clustered regularly interspaced short palindromic repeats testing in one pot (CRISPR-top) assay which detected fluid-handling step tube. The reaction was performed isothermally within 1 h; thus,...
Nocardia is a complex and diverse genus of aerobic actinomycetes that cause clinical presentations, which are difficult to diagnose due being misunderstood. To date, the genetic diversity, evolution, taxonomic structure still unclear. In this study, we investigated pan-genome 86 type strains clarify their diversity. Our study revealed an open for containing 265,836 gene families, with about 99.7% variable. Horizontal transfer appears have been important evolutionary driver diversity shaping...
Nocardia, a rare but potentially fatal pathogen, can induce systemic infections with diverse manifestations. This study aimed to investigate the tissue and organ damage caused by Nocardia farcinica (N. farcinica) in mice via different infection routes, evaluate resulting host immune responses, assess its invasiveness brain tissue.
Nocardia cyriacigeorgica is a common etiological agent of nocardiosis that has increasingly been implicated in serious pulmonary infections, especially immunocompromised individuals. However, the evolution, diversity, and pathogenesis N. have remained unclear. Here, we performed comparative genomic analysis using 91 strains, 45 which were newly sequenced this study. Phylogenetic average nucleotide identity (ANI) analyses revealed contained five species-level clades (8.6 to 14.6% interclade...
Under the COVID-19 pandemic background, nucleic acid detection has become gold standard to rapidly diagnose infectious disease. A rapid, low cost, reliable platform will be key control next potential pandemic. In this study, a platform, which combined CRISPR/Cas12a-based with loop-mediated isothermal amplification (LAMP), was developed and termed CRISPR-CLA. CRISPR-CLA system, LAMP preamplification employed, used monitor preamplicons. The forward inner primer (FIP) engineered protospacer...
Abstract Background Pulmonary infections caused by non-diphtheriae corynebacteria are increasing. However, rapid identification of Corynebacterium species poses a challenge due to the low genetic variation within genus. Methods Three reference strains and 99 clinical isolates were used in this study. A qPCR followed high-resolution melting (HRM) targeting ssrA was performed simultaneously identify C. striatum , propinquum simulans . To further evaluate assay’s performance, 88 sputum samples...
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ABSTRACT Nocardia farcinica can cause a rare, yet potentially fatal, central nervous system infection. NbtS protein may be key virulence factor in N. infection of the brain. In this study, we investigated function virulence-associated microglial cells vitro and infected mice vivo . We explored interactions between (BV2 human clone 3), revealing that activates toll-like receptor 4-dependent MyD88-IRAK4-IRAK1 MAPK/nuclear kappa B (NF-κB) pathways, significantly enhancing pro-inflammatory...
Abstract Background Nocardia is a facultative intracellular pathogen that infects the lungs and brains of immunocompromised patients with consequences can be fatal. The incidence such infections rising, immunocompetent individuals are also being infected, there need to learn more about this neglected bacterial interaction its human host. Results We have applied dual RNA-seq assess global transcriptome changes occur simultaneously in farcinica ( N. ) infected epithelial alveolar host cells,...
Abstract Aims To establish a CRISPR-based nucleic acid detection platform and apply it to the of Nocardia farcinica. Methods Results A platform, termed CRISPR-CPA (CRISPR/Cas12a combined with PCR amplification), which employed for pre-amplification target sequences CRISPR-Cas12a-based decoding amplicons, was developed. demonstrate its feasibility, applied N. pair primers crRNA, targeting conservative specific part gyrA farcinica reference strain IFM 10152, were designed according principle...
The mechanism underlying the pathogenesis of Nocardia is not fully known. Nfa34810 protein farcinica has been predicted to be a virulence factor. However, relatively little known regarding interaction with host cells, specifically invasion and innate immune activation. In this study, we aimed determine role recombinant during infection. We demonstrated that an immunodominant located in cell wall. was able facilitate uptake internalization latex beads coated into HeLa cells. Furthermore,...
Abstract The mammalian cell entry (Mce) family of proteins consists invasin-like membrane-associated proteins. roles Mce1C and Mce1D in host–pathogen interactions have not been investigated. In this study, we demonstrate that protein is localized the wall fraction N. farcinica . Both are expressed at level mRNA elicit antibody responses during infection. facilitate internalization Escherichia coli expressing or latex beads coated with by HeLa cells, respectively. We further can suppress...
A novel bacterial strain, CDC141T, was isolated from sputum samples of a patient with pulmonary infection in Hainan Province, PR China. We performed polyphasic study to assess the taxonomic position new species. Based on results 16S rRNA gene sequence analyses, strain CDC141T belonged genus Nocardia highest similarity nova NBRC 15556T (98.84 %) and macrotermitis RB20T (98.54 %). The dapb1 sequence-based phylogenetic phylogenomic trees further showed that clustered distinct clade adjacent...
Nocardia is emerging as a serious and easily neglected pathogen in clinical practice with multidrug resistance that extends the treatment period for months or even years. This has led to investigation of vaccine approach prevent infections. However, studies on protective proteins have not yet been carried out. In present work, over 500 supernatant N. farcinica IFM10152 were identified by LC−MS/MS. silico analysis these high content (score > 2000) predicted NFA49590 was one conserved strains...
Increasing the ratio of internal and external surface areas heat exchangers is ordinarily considered to be an effective way improving performance. In this paper, a novel finless exchanger with mini-diameter tubes used as air heater or cooler proposed. order fully understand performance type exchanger, test bench was established. The heating cooling performances were tested according relevant standard specifications. Furthermore, compared seven conventional exchangers. This has excellent...
Currently, there is no established protocol for the long-term performance monitoring of air-to-air heat pumps. This study aims to address critical issues and validate accuracy virtual flow meter formed by temperature difference in practical measurements heating capacity pumps field. The enthalpy approach was used on refrigerant side air conditioner assess condenser. These findings suggest that field technique introduces a certain level measurement error when estimating mass rate....
ISCR28 is a fully functional and active member of the IS91-like family insertion sequences. 1,708-bp long contains 1,293-bp putative open reading frame that codes transposase. Sixty ISCR28-containing sequences from GenBank generated 27 non-repeat genetic contexts, all which represented naturally occurring biological events had occurred in wide range gram-negative organisms. Insertion into target DNA preferred presence 5′-GXXT-3′ sequence at its terIS (replication terminator) end. Loss first...