To investigate the characteristics and prognostic factors in elderly patients with COVID-19.Consecutive cases over 60 years old COVID-19 Renmin Hospital of Wuhan University from Jan 1 to Feb 6, 2020 were included. The primary outcomes death survival till March 5. Data demographics, clinical features, comorbidities, laboratory tests complications collected compared for different outcomes. Cox regression was performed factors.339 (aged 71±8 years,173 females (51%)) enrolled, including 80...

Significance Cholera, a major disease in human history, has terrorized the world through seven pandemics. The seventh pandemic started Indonesia 1961 and spread globally, currently infecting 3–5 million people annually. By combining all available historical records genomic analysis of preseventh some early strains, we revealed complex six-step evolution strain from its probable origin South Asia to nonpathogenic form Middle East ∼1900 ∼1925, where it evolved into before becoming widespread...

As a core microbe of the human gut ecosystem, Bacteroides vulgatus has been linked to multiple aspects metabolic disorders in collection associative studies, which, while indicative, warrants more direct experimental evidence verify. In this study, we experimentally demonstrated that oral administration B. Bv46 ameliorated serum lipid profile and systemic inflammation high-fat diet-induced hyperlipidemic rats microbiome-regulated manner, which appears be associated with changes bile acid...

We have sequenced the gene clusters for type strains of Acinetobacter baumannii serotyping scheme developed in 1990s, and used sequences to better understand diversity surface polysaccharides genus. obtained genome 27 available serovar strains, identified 25 polysaccharide cluster sequences. There are structures 12 these polysaccharides, general genes present appropriate structure where known. This greatly facilitates interpretation. also find 53 different glycosyltransferase genes, 7 can...

is one of the predominant

ABSTRACT The commensal bacterium Alistipes shahii is a core microbe of the human gut microbiome and its abundance negatively correlated with inflammatory bowel diseases (IBDs). However, fundamental role in regulating response remains unknown. Using dextran sulfate sodium (DSS)-induced colitis mouse model, we examined effect A. strain As360 intervention on host found that alleviated disease activity index, colon shortening, colonic histopathological lesion. levels tight junction proteins...

Prolonged prothrombin time at admission predicts poor clinical outcome in COVID-19 patients

ABSTRACT High-quality draft genomes of six Salmonella enterica subspecies strains from Cambodian poultry marketplaces were sequenced. The identified as Corvallis-, Monschaui-, and Kentucky-serovars. fluoroquinolone resistance gene, qnrS, was found in three different provinces. These serve references for antibiotic genotypes.

ABSTRACT Coastal water, sediment, and algae samples were collected from St. John’s Island, Singapore, enriched in either broth or agar. Metagenomic sequencing was carried out on DNA these enrichments analyzed. A total of 29 metagenome-assembled genomes had been successfully asserted to be a close representation Shewanella .

ABSTRACT Two Ruegeria strains, SCP10 ( JBDZYF000000000 ) and SCP11 JBDZYG000000000 ), were isolated from coral tissue skeletal macerates of the resilient Pachyseris speciosa in Singapore. We present genomes these isolates, which contain genes involved dimethylsulfoniopropionate metabolism denitrification, good attributes for candidate probiotics.

Marine heterotrophic bacteria in coastal waters respond to the influx of carbon from natural and anthropogenic sources. We identified two nearly identical, (99.9% average nucleotide identity; 100% amino acid same DNA G + C content 52.3 mol%) high-quality (≥99% CheckM completeness ≤ 1.3% contamination) draft metagenome-assembled genomes (MAGs; SJ0813 SJ0972) seawater microbiomes a southern island Singapore that is protected marine park. The MAGs were only assigned Cellvibrionaceae family...

Objectives This prospective study was carried out to investigate molecular characteristics and antimicrobial susceptibility patterns of Citrobacter spp. from extraintestinal infections. Methods Forty-six clinical isolates were isolated hospital patients with infections analyzed by multilocus sequence typing (MLST) using seven housekeeping genes. Antimicrobial testing performed disk diffusion method according the Clinical Laboratory Standards Institute (CLSI) recommendations. Adhesion...

Gut microbiome is of major interest due to its close relationship health and disease. Bacteria usually vary in gene content, leading functional variations within species, so resolution higher than species-level methods needed for ecological clinical relevance. We design a protocol identify strains selected species with high discrimination numbers by amplicon sequencing the flagellin gene. apply fecal samples from human diet trial, targeting Escherichia coli. Across 119 16 individuals, there...

Abstract An ideal bacterial phylogenetic tree accurately retraces evolutionary history and incorporates mutational, recombination other events on the appropriate branches. Current strain-level analysis based large numbers of genomes lacks reliability resolution, is hard to be replicated, confirmed reused, because highly divergent nature microbial genomes. We present SNPs Recombination Events Tree (SaRTree), a pipeline using six “living trees” modules that addresses problems arising from high...

Food supply is one of the major drivers animal behavior, and gut microbiome an important mediator between food its effects on physiology. However, predicting outcome diet change consequences for has proven extremely challenging. We propose this reflects processes occurring at different scales. Inadequate accounting multi-level complexity nutrition (nutrients, foods, diets) obscures influence subsequently animal. Here, we present a detailed year-round, analysis changes in wild population...

ABSTRACT A Pseudovibrio sp. was isolated from the skeleton of heat resilient coral Pachyseris speciosa . Genome analysis revealed presence complete denitrification pathway and potential dimethylsulfoniopropionate metabolism which enhance resilience production tropodithietic acid, an antibiotic implicated in host defense.

Salmonella enterica serovar Enteritidis is a major cause of foodborne infections and outbreaks in humans. Effective surveillance timely outbreak detection are essential for public health control. Multilevel genome typing (MGT) with multiple levels resolution has been previously demonstrated as promising tool this purpose. In study, we developed MGT nine S. characterised the genomic epidemiology detail. We examined 26 670 publicly available sequences from isolates spanning 101 years 86...

Bordetella pertussis causes (or whooping cough), a severe respiratory infectious disease in infants, although it can be prevented by whole cell and acellular vaccines. The recent resurgence industrialised countries is partly attributed to pathogen adaptation vaccines, while emergence of antimicrobial resistance, specifically macrolides China, has become concern. Surveillance current circulating emerging strains therefore vital understand the risks they pose public health. Although use...

Flagellin, the agent of prokaryotic flagellar motion, is very widely distributed and H antigen serology. Flagellin molecules have a variable region that confers serotype specificity, encoded by middle gene, also conserved regions two ends gene. We collected all available flagellin protein sequences found diversity to be at levels. In each species investigated, there are hypervariable (HVR) forms without detectable homology in between them. There considerable variation within HVR forms,...

ABSTRACTWhooping cough (pertussis) is a highly contagious respiratory disease caused by the bacterium Bordetella pertussis. Despite high vaccine coverage, pertussis has re-emerged in many countries including Australia and two large epidemics since 2007. Here, we undertook genomic phylogeographic study of 385 Australian B. isolates collected from 2008 to 2017. The population was found be composed mostly ptxP3 strains carrying different fim3 alleles, with ptxP3-fim3A genotype expanding far...

is the dominant species of

Abstract Summary The reliable and timely recognition of outbreaks is a key component public health surveillance for foodborne diseases. Whole genome sequencing (WGS) offers high resolution typing bacterial pathogens facilitates the accurate detection outbreaks. This relies on grouping WGS data into clusters at an appropriate genetic threshold. However, methods tools selecting adjusting such thresholds according to required epidemiological context are lacking. Here we present DODGE (Dynamic...

The 7th cholera pandemic began in 1961 Sulawesi, Indonesia, and then spread around the world at least three waves. However, lack of genome sequences for Vibrio cholerae strains under long-term surveillance East Asia, especially China, has restricted our understanding dynamics intracountry intercountry evolution transmission 7th-pandemic clones. In this study, we obtained 60 V. isolated Shanghai, largest port city from to 2011. Our whole-genome-based phylogeny revealed that all but one fell...

Cholera caused by Vibrio cholerae O139 was first reported in Bangladesh and India 1992. To determine the genomic epidemiology origins of China, we sequenced 104 isolates collected from Zhejiang Province, during 1994-2018 compared them with 57 genomes other countries Asia. Most fell into 3 clusters (C1-C3), which probably originated (C1) Thailand (C2 C3) early 1990s. Different harbored different antimicrobial resistance genes IncA/C plasmids. The integrative conjugative elements carried were...