- Genetic Mapping and Diversity in Plants and Animals
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Plant Reproductive Biology
- Rice Cultivation and Yield Improvement
- Genetics and Plant Breeding
- Plant Stress Responses and Tolerance
- GABA and Rice Research
- Heavy metals in environment
- Heavy Metals in Plants
- Genetic and phenotypic traits in livestock
- Plant Micronutrient Interactions and Effects
- Insect-Plant Interactions and Control
- Trace Elements in Health
- Plant Virus Research Studies
- Transgenic Plants and Applications
- Plant Parasitism and Resistance
- CRISPR and Genetic Engineering
- Plant biochemistry and biosynthesis
- Heat shock proteins research
- Aluminum toxicity and tolerance in plants and animals
- Cocoa and Sweet Potato Agronomy
- Heavy Metal Exposure and Toxicity
- Genetics, Aging, and Longevity in Model Organisms
- Plant Gene Expression Analysis
China National Hybrid Rice R&D Central Hunan Hybrid Rice Reserch Center
2014-2025
Hunan Academy of Agricultural Sciences
2017-2025
Hunan University
2025
State Key Laboratory of Hybrid Rice
2014-2024
Rice University
2014
University of Chinese Academy of Sciences
2012
Institute of Subtropical Agriculture
2012
Chinese Academy of Sciences
2012
Heterosis utilization is the most effective way to improve rice yields. The cytoplasmic male-sterility (CMS) and photoperiod/thermosensitive genic (PTGMS) systems have been widely used in production. However, rate of resource for CMS system hybrid low, seed production PTGMS affected by environment. technical limitations these two breeding methods restrict rapid development rice. advantages (GMS) rice, such as stable sterility free combination, can fill gaps first generations technology. At...
The discovery of a wild abortive-type (WA) cytoplasmic male sterile (CMS) line and breeding its restorer have led to the commercialization three-line hybrid rice, contributing considerably global food security. However, molecular mechanisms underlying fertility abortion restoration CMS-WA lines remain largely elusive. In this study, we cloned gene, Rf20, following genome-wide association study analysis core parent rice. We found that Rf20 was present in all parental lines, but different...
OsNramp5 is a key gene involved in the control of uptake Cd, Mn, and other metal ions by rice root cells. The functional deficiency this can significantly reduce accumulation Cd grains, but effects its mutation on agronomic traits such as yield quality have not been investigated comprehensively yet. In present study, three Huanghuazhan-based mutants [LCH1 (Low Cadmium Huanghuazhan 1), LCH2 2), LCH3 3)] were obtained using clustered regularly interspaced short palindromic...
The P-type pentatricopeptide repeat (PPR) proteins are crucial for RNA editing and post-transcriptional regulation in plant organelles, particularly mitochondria. This study investigates the role of OsPPR674 rice, focusing on its function mitochondrial editing. Using CRISPR/Cas9 technology, we generated ppr674 mutant examined phenotypic molecular characteristics. results indicate that exhibits reduced height, decreased seed-setting rate, poor drought tolerance. Further analysis revealed...
Copper is a mineral element essential for the normal growth and development of plants; however, excessive levels can severely affect plant development. Oryza sativa L. multiple stress-responsive gene 3 (OsMSR3) small, low-molecular-weight heat shock protein (HSP) gene. A previous study has shown that OsMSR3 expression improves tolerance Arabidopsis to cadmium stress. However, role in Cu stress response plants remains unclear, and, thus, this aimed elucidate phenomenon thaliana, further...
Abstract Seed setting rate is one of the critical factors that determine rice yield. Grain formation a complex biological process, whose molecular mechanism yet to be improved. Here we investigated function an OVATE family protein, Embryo Sac Development 1 (ESD1), in regulation seed (Oryza sativa) by examining its loss-of-function mutants generated via clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated9 (Cas9) technology. ESD1 was predominantly expressed at...
Abstract Genetic improvement of grain quality is more challenging in hybrid rice than inbred due to additional nonadditive effects such as dominance. Here, we describe a pipeline developed for joint analysis phenotypes, effects, and generations (JPEG). As demonstration, analyze 12 traits 113 lines (male parents), five tester (female 565 (113×5) their hybrids. We sequence the parents single nucleotide polymorphisms calling infer genotypes Genome-wide association studies with JPEG identify 128...
The genomic expression profile of the super-hybrid rice Liangyoupeijiu female parent Pei'ai 64S in different tissues at developmental stages under low temperature, drought, and high temperature stresses were detected using an Affymetrix GeneChip Rice Genome Array to screen upregulated downregulated genes.In this study, we screened drought-resistant gene OsRCI2-5, after which a constitutive OsRCI2-5 construct was created transferred into Nipponbare.After polyethylene glycol-6000 drought...
Abstract Rice yield per plant has a complex genetic architecture, which is mainly determined by its three component traits: the number of grains panicle (GPP), kilo-grain weight (KGW), and tillers (TP). Exploring ideotype breeding based on selection for genetically less traits an alternative route further improving rice production. To understand basis relationship between traits, we investigated four two hybrid populations (575 + 1495 F 1 ) in different environments conducted meta-analyses...
With 6 figures and 4 tables Abstract Heterosis refers to the phenomenon in which hybrid progeny of two inbred varieties exhibits enhanced growth or agronomic performance. Although a century‐long history research has generated several hypotheses regarding genetic basis heterosis, molecular mechanisms underlying heterosis heterotic gene activity remain elusive. In this study, rice crosses PA64S×93‐11 Y58S×93‐11 were used as plant materials analyse activities 10 abiotic stress‐responsive genes...
Plant proline-rich proteins (PRPs) are cell wall that widely distributed in plants. Previous studies have shown these play a crucial role adversity stress processes, but their function the regulation of pollen fertility rice remains unknown. In this study, we identified OsPRP1 contains Pollen_Ole-e_I allergenic structural domain, obtained quadruple mutant named osprp1.1/1.2/1.3/1.4, and observed significant reductions fertility, seed-setting rates, deformation collapse microspores during...
<title>Abstract</title> This study pioneered the identification method of cadmium accumulation in rice grains sterile lines, took lead discovering important resources low Luohong 3A and 4A, established precise design breeding technology system rice, developed first variety approved by state "Xizi 3", which is great significance for solving problem excessive southern China. It was selected as major leading agricultural Ministry Agriculture Rural Affairs 2024 new scientific technological...
Summary Yield level and grain quality determine the commercial potential of rice ( Oryza sativa L.) varieties. Mining using genes that control important characteristics are major tasks for plant breeders. Here, a genome-wide association study was conducted to genetic bases 12 traits in 113 varieties 565 testcross hybrids. A total 56 significant SNPs were associated with 9 variety phenotypic, general combining ability, hybrid phenotypic mid-parental heterotic datasets, from which 45 novel...
Abstract Rice floral organs are closely related to rice yield and quality, MADS family genes play an important role in organ development. In this study, we identified functions of two development by bioinformatics analysis CRISPR/Cas9 technology. Our results showed that MADS6 MADS17 were a pair highly homologous genes, which had similar sequences, motifs expression profiles. The mads6 / 17 mutants abnormal, whereas mads17 no difference with wild type. Collectively, these reveal there...
Abstract Rice is subjected to various abiotic environmental stress throughout its lifetime and it has formed a complex regulatory network that balances growth development with response. A stress-responsive gene, OsSUI1 , was previously mined from microarray data, transgenic studies revealed overexpression of this gene caused abnormal floral organ development. However, the role in response how regulated unclear. In study, we obtained ossui1 mutant by editing, exhibited high sensitivity cold,...
As one of the important factors rice yield, grains number per panicle has gained wide attention from breeders. The formation is a complex biological process, which controlled by many factors, such as spike type, spikelet differentiation rate, and etc. With development plant functional genomics-related techniques, some progress been made in cloning gene study its mechanism. Recently, mechanism genes controlling grain great progress. In this review, we summarize systematically recent research...