A5014085609- Marine and coastal ecosystems
- Marine Toxins and Detection Methods
- Mass Spectrometry Techniques and Applications
- Aquatic Ecosystems and Phytoplankton Dynamics
- Metabolomics and Mass Spectrometry Studies
- Adipose Tissue and Metabolism
- Genetic Mapping and Diversity in Plants and Animals
- Analytical Chemistry and Chromatography
- Lipid metabolism and biosynthesis
- Biocrusts and Microbial Ecology
- Advanced Proteomics Techniques and Applications
- Nicotinic Acetylcholine Receptors Study
- Celiac Disease Research and Management
- Microwave-Assisted Synthesis and Applications
- Birth, Development, and Health
- Metabolism and Genetic Disorders
- Marine and coastal plant biology
- Pediatric Hepatobiliary Diseases and Treatments
- Redox biology and oxidative stress
- Ion-surface interactions and analysis
- Coral and Marine Ecosystems Studies
- Chemical Synthesis and Analysis
- Food Allergy and Anaphylaxis Research
- Isotope Analysis in Ecology
- Monoclonal and Polyclonal Antibodies Research
North Carolina State University
2020-2025
Center for Food Safety and Applied Nutrition
2015-2020
United States Food and Drug Administration
2015-2020
University of Florida
2009-2013
Native Americans domesticated maize ( Zea mays ssp. ) from lowland teosinte parviglumis parviglumis) in the warm Mexican southwest and brought it to highlands of Mexico South America where was exposed lower temperatures that imposed strong selection on flowering time. Phospholipids are important metabolites plant responses low-temperature phosphorus availability have been suggested influence Here, we combined linkage mapping with genome scans identify High PhosphatidylCholine 1 HPC1 ), a...
The identification of a new suite toxins, called azaspiracids (AZA), as the cause human illnesses after consumption shellfish from Irish west coast in 1995, resulted interest understanding global distribution these toxins and species small dinoflagellate genus Azadinium, known to produce them. Clonal isolates four A. poporum, cuneatum, obesum dalianense were obtained incubated sediment samples collected Puget Sound, Washington State 2016. These Azadinium identified using morphological...
Infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) was coupled with high-resolution accurate-mass-mass spectrometry (HRAM-MS) to image perfluoroalkyl and polyfluoroalkyl substances (PFAS) in stabilized soil cores. Previous field-scale research demonstrated a substantial decrease the leachability of PFAS following application situ stabilization solidification (S/S) an aqueous film-forming foam (AFFF) source zone. While this previous study empirically confirmed...
Although acute myocardial infarction (MI) is consistently among the top causes of death in United States, spatial distribution lipids and metabolites following MI remains to be elucidated. This work presents investigation an vivo rat model using mass spectrometric imaging (MSI) multivariate data analysis. MSI was conducted on cardiac tissue a 24-h left anterior descending coronary artery ligation analyze multiple compound classes. First, small metabolite, creatine, used identify areas...
The cyanobacterium Aphanizomenon flos-aquae (AFA), from Upper-Klamath Lake, Oregon, are used to produce blue-green algal (BGA) dietary supplements. periodic co-occurrence of hepatotoxin-producing contaminant species prompted the Oregon Health Division establish a limit 1 μg/g microcystin (MC) for products sold in 1997. At federal level, current good manufacturing practice (CGMP) regulations supplements require manufacturers specification, and test, limits on contaminants that may adulterate...
The zebrafish (
Microcystins (MCs) are hepatotoxic heptapeptides produced by cyanobacteria and potent inhibitors of protein phosphatases in eukaryotic cells. Algae for dietary supplements harvested from outdoor environments can be contaminated with MCs. Monitoring MCs these products is necessary but complicated their structural diversity (>250 congeners). We used a combination phosphatase inhibition assay (PPIA), ELISA, LC−MS/MS, nontargeted LC–high-resolution MS (LC–HRMS) thiol derivatization to...
A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the simultaneous detection and quantitation of seven microcystin congeners (1–7) nodularin-R (8) in blue-green algal dietary supplements. Single-laboratory validation data were collected four supplement matrices (capsule, liquid, powder, tablet) fortified at toxin concentrations from 0.25–2.00 μg/g (ppm). Average recoveries relative standard deviations (RSD) using matrix-corrected solvent calibration curves...
Due to the increasing prevalence of Dinophysis spp. and their toxins on every US coast in recent years, need identify monitor for problematic populations has become apparent. Here, we present morphological analyses, using light scanning electron microscopy, rDNA sequence analysis, a ~2-kb ribosomal ITS1, 5.8S, ITS2, LSU DNA, collected mid-Atlantic estuarine coastal waters from Virginia New Jersey better characterize local populations. In addition, analyzed diarrhetic shellfish poisoning...
Derivatization of steroids is typically required before analysis by gas chromatography/mass spectrometry (GC/MS); nevertheless, the derivatization process can often be time-consuming and irreproducible. Although several strategies have been employed to enhance this process, few potential microwave-accelerated (MAD) more efficient than traditional thermal methods. MAD using a synthesis microwave system was evaluated compared methods in terms yield, reproducibility, overall time. Parameters...
The effectiveness of a proline endopeptidase (PEP) in hydrolyzing gluten and its putative immunopathogenic sequences was examined using antibody-based methods mass spectrometry (MS). Based on the results methods, fermentation wheat containing sorghum beer resulted reduction detectable concentration. addition PEP further reduced Only one sandwich ELISA able to detect apparent low levels present beers. A competitive pepsin-trypsin hydrolysate calibrant unreliable because peptide profiles beers...
Abstract Native Americans domesticated maize ( Zea mays ssp. ) from lowland teosinte parviglumis in the warm Mexican southwest and brought it to highlands of México South America where was exposed lower temperatures that imposed strong selection on flowering time. Phospholipids are important metabolites plant responses low-temperature phosphorus availability, have been suggested influence Here, we combined linkage mapping with genome scans identify High PhosphatidylCholine 1 HPC1 ), a gene...
Phospholipid oxidation has been implicated in the pathogenesis and progression of numerous age-related neurodegenerative diseases. Despite these implications, this broad class biomolecules remains poorly characterized. In work, fragmentation patterns [M + H](+) Na](+) ions intact phosphatidylcholine products (OxPCs) were characterized by matrix-assisted laser desorption/ionization tandem mass spectrometry (MALDI MS(n), n = 2, 3, 4). MS(2) both short-chain OxPCs yielded product related to PC...
Vitamin D is well known for its traditional role in bone mineral homeostasis; however, recent evidence suggests that vitamin also plays a significant metabolic control. This study served to investigate putative linkages between deficiency (VDD) and disruption of bioactive lipids by MS imaging. Our approach employed infrared-matrix-assisted laser desorption electrospray ionization imaging lipid metabolite profiling 6-month-old zebrafish fed either VDD or D-sufficient (VDS) diet. Using...
ABSTRACT Malrotation of the intestine is a prevalent birth anomaly, etiology which remains poorly understood. Here, we show that late-stage exposure Xenopus embryos to atrazine, widely used herbicide targets electron transport chain (ETC) reactions, elicits intestinal malrotation at high frequency. Interestingly, atrazine specifically inhibits cellular morphogenetic events required for gut tube elongation, including cell rearrangement, differentiation and proliferation; insufficient...
Dihydrodinophysistoxin-1 (dihydro-DTX1, (M-H)−m/z 819.5), described previously from a marine sponge but never identified as to its biological source or in shellfish, was detected multiple species of commercial shellfish collected the central coast Gulf Maine, USA 2016 and 2018 during blooms dinoflagellate Dinophysis norvegica. Toxin screening by protein phosphatase inhibition (PPIA) first presence diarrhetic poisoning-like bioactivity; however, confirmatory analysis using liquid...