A5089098214- Animal Disease Management and Epidemiology
- T-cell and Retrovirus Studies
- Vector-Borne Animal Diseases
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Influenza Virus Research Studies
- Viral Infections and Vectors
- CRISPR and Genetic Engineering
- Innovative Microfluidic and Catalytic Techniques Innovation
- Virus-based gene therapy research
- Cytomegalovirus and herpesvirus research
- Microbial infections and disease research
- Biosensors and Analytical Detection
- Bacteriophages and microbial interactions
- Vibrio bacteria research studies
- Metallurgy and Material Science
- Milk Quality and Mastitis in Dairy Cows
- Metallurgy and Material Forming
- Galectins and Cancer Biology
- Identification and Quantification in Food
- COVID-19 epidemiological studies
- Viral Infections and Immunology Research
University of Miyazaki
2016-2025
Hokkaido University
2019-2021
Jeonbuk National University
2016
Bovine viral diarrhea (BVD) is caused by the BVD virus (BVDV) and has been reported worldwide in cattle. To estimate BVDV circulation among cattle where few cases were southern Japan, 1910 serum samples collected from 35 farms without a outbreak investigated to detect antibodies against BVDV-1 BVDV-2 using an indicator with cytopathogenic effect luciferase gene, respectively. Neutralizing detected more frequently 18 vaccinated than 17 nonvaccinated farms. In farms, 9.6%, 1.8%, 13.8% of...
Abstract Background While early detection and containment are key to controlling the African swine fever (ASF) pandemic, lack of practical testing methods for use in field a major barrier achieving this feat. Objectives To describe development rapid sensitive point‐of‐care test (POCT) ASF, its evaluation using whole blood samples settings. Methods In total, 89 were collected from Vietnamese farms performed POCT combination crude DNA extraction LAMP (loop‐mediated isothermal amplification)...
Porcine epidemic diarrhoea (PED) is an emerging disease in pigs that causes massive economic losses the swine industry, with high mortality suckling piglets. Early identification of PED virus (PEDV)-infected herd through surveillance or monitoring strategies necessary for mass control PED. However, a common working diagnosis system involves identifying PEDV-infected animals individually, which costly and time-consuming approach. Given above information, thrusts this study were to develop...
Enzootic bovine leukosis is a lethal neoplastic disease caused by leukemia virus (BLV), belongs to family Retroviridae. The BLV proviral load (PVL) represents the quantity of genome that has integrated into host’s in BLV-infected cells. Bovine leukocyte antigen (BoLA) class II allelic polymorphisms are associated with PVLs cattle. We sought identify relationships between BoLA-DRB3 heterozygosity and among different cattle breeds. Blood samples from 598 were quantified determine their...
The cattle industry is suffering economic losses caused by bovine leukemia virus (BLV) and enzootic leukosis (EBL), the clinical condition associated with BLV infection. This pathogen spreads easily without detection farmers veterinarians due to lack of obvious signs. Cattle movement strongly contributes inter-farm transmission BLV. study quantified farm-level risk introduction using a analysis. A generalized linear mixed model predicting proportion BLV-infected was constructed based on...
In the transmission control of chronic and untreatable livestock diseases such as bovine leukemia virus (BLV) infection, removal viral superspreaders is a fundamental approach. On other hand, selective breeding cattle with BLV-resistant capacity also critical for reducing damage to productivity by keeping infected cattle. To provide way measuring BLV proviral load (PVL) identifying susceptible/resistant simply rapidly, we developed fourplex droplet digital PCR method targeting pol gene,...
Abstract Bovine viral diarrhea (BVD), caused by bovine virus (BVDV), has a significant economic impact on affected farms worldwide. For effective disease control, it is crucial to select an appropriate vaccine based the specific genotype of BVDV. Therefore, developing rapid and reliable assay detect BVDV imperative for controlling spread disease. In this study, we developed TaqMan types 1 2 directly in serum without extraction RNA. The direct effectively detected both BVDV1 BVDV2 with...
The purpose of this study was to detect porcine epidemic diarrhea virus (PEDV) subclinically infected pigs shipped from non-case farms slaughterhouses. Systematic sampling conducted at two A total 1,556 blood samples were collected 80 case and over 6 months old. Blood centrifuged obtain sera. Serial serum dilutions subjected serological examination for PEDV presence using Neutralization test (NT). cut-off titer set 1:2 dilution with least one positive sample in duplicate classified as...
Highly pathogenic avian influenza (HPAI) outbreaks engender a severe economic impact on the poultry industry and public health. Migratory waterfowl are considered natural hosts of HPAI virus, viruses known to be transmitted over long distances during seasonal bird migration. Bird migration is greatly affected by weather. Many studies have shown relationship between either autumn or spring climate. However, few annual This study aimed establish model for number migratory involved in virus...
As genetically resistant individuals, the "elite controllers" (ECs) of human immunodeficiency virus infection have been focused on as keys to developing further functional treatments in medicine. In livestock production field, identifying ECs bovine leukemia (BLV) cattle is desired stop BLV transmission chains farms. Cattle carrying leukocyte antigen (BoLA)-DRB3*009:02 allele (DRB3*009:02) a strong possibility being ECs. Most this maintain undetectable proviral loads and do not shed even...
Bovine viral diarrhea virus (BVDV) footprint has spread across the globe and is responsible for one of most economically important diseases in cattle. In Japan, some regional surveillance preventive measures to control bovine (BVD) have been implemented. However, BVDV infection poorly understood cattle industries, there no systematic BVD system program. Kyushu center raising beef Japan. Therefore, this study aimed determine using a slaughterhouse survey among Kyushu, A total 1,075 blood...
Bovine viral diarrhea (BVD) caused by BVD virus (BVDV) leads to economic loss worldwide. Cattle that are persistently infected (PI) with BVDV known play an important role in transmission association the animal movement, as they shed during their lifetime. In this research, “hot spot” for was estimated combining phylogenetic and epidemiological analyses PI cattle lived together on affected farms Tokachi district, Hokkaido prefecture, Japan. Viral isolates were genetically categorized into...
<title>Abstract</title> Bovine viral diarrhea (BVD), caused by bovine virus (BVDV), has a significant economic impact on affected farms worldwide. For effective disease control, it is crucial to select appropriate vaccinations based the specific genotype of BVDV. Therefore, developing rapid and reliable assay detect BVDV imperative control spread disease. In this study, we developed direct TaqMan type 1 2 from serum. The effectively detected both BVDV1 BVDV2 with confirmed specificity showed...
Most bovine leukemia virus (BLV)-infected cattle do not have clinical signs (aleukemic AL), but some develop persistent lymphocytosis (PL) and B-cell lymphosarcoma (enzootic leucosis [EBL]). BLV infection is a well-known cause of chronic wasting disease, which associated with reduction in milk productivity immunity dairy cattle. However, the effect on beef clear. The objective this study was to investigate slaughtered A total 997 blood samples were collected from 2 slaughterhouses Miyazaki...
The bovine leukocyte antigen (BoLA) DRB3*009:02 allele is strongly associated with a low/undetectable leukemia virus (BLV) proviral load. Understanding the status of cattle possessing key for BLV control by breeding. We performed survey DRB3*009:02-carrying in two prefectures Japan using TaqMan assay developed previously. was found 3.8% (confidence interval (CI): 3.3-4.3) 6020 Japanese Black female cattle. A prefecture-level difference found: observed 8.6% CI: 7.5-9.9) 2242 birth prefecture...
Background] In January 2014, a suspect case of highly pathogenic avian influenza (HPAI), with increasing mortality and decreasing egg production in flock, was notified at breeder duck farm Gochang, Jeollabuk-do province.An infection HPAI virus type H5N8 confirmed next day, total 212 outbreaks were during the subsequent 193 days.This study investigated clinical characteristics observed Korean poultry farms 2014 epidemic.[Materials Methods] History manifestation signs by time hour.Data...
Volume 8, No. 1, e00493-22, 2023, http://doi.org/10.1128/msphere.00493-22. Page 7, Fig. 4: We found errors in the significance indicator lines of this figure. In particular, there is no significant difference percentage BLV-infected cells between *016:01/*009:02 group and *009:02/other allele group. Instead, a other alleles note that does not affect overall discussion conclusions. The corrected 4 shown below.
Abstract Aim of our study is to establish models for predicting the number migratory wild birds based on meteorological data. From 136 species birds, which have been observed at Futatsudate in Miyazaki, Japan, from 2008 2016, we selected potential high-risk species, can introduce highly pathogenic avian influenza (HPAI) virus into Miyazaki; defined them as “risky birds”. We then performed regression analysis model relationship between risky and 10 bird birds: Mallard ( Anas platyrhynchos ),...
Abstract A combined host biomarker and pathogen diagnosis provides insight into disease progression risk contributes to appropriate clinical decision-making regarding prevention treatment. In preventive veterinary medicine, such could improve risk-based livestock herd management. We developed a single-well based test for of bovine leukemia virus (BLV) MHC ( BoLA )- DRB3 alleles. fourplex droplet digital PCR method targeting the BLV pol gene, BLV-susceptible DRB3*016:01 allele, resistant...