A5074235895- Pregnancy and preeclampsia studies
- Acute Ischemic Stroke Management
- Neurological Complications and Syndromes
- Nitric Oxide and Endothelin Effects
- Neurological Disease Mechanisms and Treatments
- Traumatic Brain Injury and Neurovascular Disturbances
- Cerebrovascular and Carotid Artery Diseases
- Birth, Development, and Health
- Neuroinflammation and Neurodegeneration Mechanisms
- Neonatal and fetal brain pathology
- Neurological Disorders and Treatments
- Barrier Structure and Function Studies
- Moyamoya disease diagnosis and treatment
- Cardiovascular Health and Disease Prevention
- Neuroscience and Neuropharmacology Research
- Reproductive System and Pregnancy
- Peroxisome Proliferator-Activated Receptors
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cerebral Venous Sinus Thrombosis
- Cardiovascular Issues in Pregnancy
- Muscle Physiology and Disorders
- Anesthesia and Neurotoxicity Research
- Mitochondrial Function and Pathology
- Pregnancy-related medical research
- Electrolyte and hormonal disorders
University of Vermont
2016-2025
Washington University in St. Louis
2024
National Institute of Neurological Disorders and Stroke
2017
Neurological Surgery
2017
National Institutes of Health
2017
Loyola University Chicago
2017
University of Vermont Medical Center
2016
University of Chicago
2011
Cambridge University Press
2008
Ospedale Bassini
1996-2002
The mechanism of action acupuncture remains largely unknown. reaction to needling known as 'de qi', widely viewed essential the therapeutic effect acupuncture, may be a key understanding its action. De qi includes characteristic sensation, perceived by patient, and 'needle grasp' acupuncturist. During needle grasp, acupuncturist feels pulling increased resistance further movement inserted needle. We hypothesize that 1) grasp is due mechanical coupling between connective tissue with winding...
Vitamin D deficiency is common among reproductive-aged women and has a role in female reproduction.This study evaluated the of 1,25-dihydroxyvitamin D3 (vit D3) ovarian follicular development steroidogenesis by using human granulosa cell (GC) model.Fifty-four who underwent vitro fertilization were enrolled.Follicular fluid (FF) mural cumulus GCs collected from small large follicles. In separate experiments, primary cultured with or without vit followed RT-PCR for mRNA expression levels. The...
We hypothesize that actin polymerization within vascular smooth muscle (VSM) in response to increased intravascular pressure is a novel and previously unrecognized mechanism underlying arterial myogenic behavior. This hypothesis based on the following observations. 1) Unlike skeletal or cardiac muscle, VSM contains substantial pool of unpolymer-ized globular (G) whose function not known. 2) The cytosolic concentration G-actin significantly reduced by an elevation pressure, demonstrating...
The objective of this study was to determine whether myogenic tone in the cerebral circulation can be modified by agents that interact with protein kinase C (PKC), a modulator intracellular calcium sensitivity. Pial arteries (194 +/- 8 microns at 125 mm Hg) were isolated from Wistar-Kyoto rats and mounted on glass microcannulas specialized arteriograph. Simultaneous recordings transmural pressure lumen diameter made video-electronic system. Myogenic tone, which developed pressures above 50...
This presentation describes structural and functional properties of the cerebral circulation that are unique to brain, an organ with high metabolic demands, need for tight water ion homeostasis. Autoregulation is pronounced in myogenic, metabolic, neurogenic mechanisms contributing maintain relatively constant blood flow during both increases decreases pressure. In addition, unlike peripheral organs where majority vascular resistance resides small arteries arterioles, large extracranial...
The role of SK(Ca) and IK(Ca) channels in myogenic tone endothelium-derived hyperpolarizing factor (EDHF) responsiveness was investigated under control conditions after ischemia reperfusion parenchymal arterioles (PA) versus middle cerebral arteries (MCA).MCA PA were dissected from male Wistar rats that ischemic for 1 hour with 24 hours (n=12) or sham controls (n=12). Basal reactivity to apamin (300 nmol/L), TRAM-34 (1.0 micromol/L), nitro-L-arginine (0.1 mmol/L) compared MCA pressurized 40...
Eclampsia, defined as unexplained seizure in a woman with preeclampsia, is life-threatening complication of pregnancy unclear etiology. Magnesium sulfate (MgSO4) the leading eclamptic prophylactic, yet its mechanism action remains unclear. Here, we hypothesized severe preeclampsia state increased susceptibility due to blood-brain barrier (BBB) disruption and neuroinflammation that lowers threshold. Further, MgSO4 decreases by protecting BBB preventing neuroinflammation. To model placental...
Ginkgo biloba extract (EGb) and alpha-lipoic acid (LA) are commercially available "antioxidant supplements" with a variety of actions that may be beneficial during acute stroke. These include inhibiting platelet leukocyte activation adhesion, reducing free radical generation, increasing cerebral blood flow. Both EGb LA have been shown to neuroprotective in cell culture global central nervous system ischemia models. In this study we investigated the efficacy clinically relevant, transient...
Circulating factors in preeclamptic women are thought to cause endothelial dysfunction and thereby contribute the progression of this hypertensive condition. Despite involvement neurological complications preeclampsia, there is a paucity data regarding effect circulating on cerebrovascular function. Using rat model pregnancy, we investigated blood-brain barrier permeability, myogenic activity, influence vasodilator mechanisms cerebral vessels exposed intraluminally plasma from normal...
The cerebral circulation has a central role in mediating the neurological complications of eclampsia, yet our understanding how pregnancy and preeclampsia affect this is severely limited. Here, we show that causes outward remodeling penetrating arterioles increased capillary density brain due to activation peroxisome proliferator-activated receptor-γ (PPARγ), transcription factor involved cerebrovascular highly activated pregnancy. Pregnancy-induced PPARγ also significantly affected...
Background and Purpose— We investigated vasoactive properties of leptomeningeal arterioles (LMAs) under normotensive conditions during hypertension aging that are known to have poor collateral flow little salvageable tissue. Methods— LMAs, identified as distal anastomotic connecting middle anterior cerebral arteries, were studied isolated pressurized from young (18 weeks) or aged (48 Wistar Kyoto (WKY18, n=14; WKY48, n=6) rats spontaneously hypertensive (SHR18, n=16; SHR48, n=6). Myogenic...
Early-onset preeclampsia (EPE) is a severe form of that involves life-threatening neurological complications. However, the underlying mechanism by which EPE affects maternal brain not known. We hypothesized plasma from women with increases blood-brain barrier (BBB) permeability vs. late-onset (LPE) or normal pregnancy (NP) and investigated its perfusing cerebral veins nonpregnant rats (n=6–7/group) human EPE, LPE, NP measuring permeability. show significantly increased BBB LPE (P<0.001)....
After focal cerebral ischemia, the function of arteries is critical to maintain cerebrovascular resistance and minimize damage ischemic brain regions during reperfusion. In this study we examined contractile isolated pressurized middle (MCAs) after 2 hours occlusion with either 1 minutes or 24 reperfusion using intraluminal suture model transient ischemia in rats.MCAs were dissected (OCC, n = 8) (RPF, 5) compared those controls that did not have surgery (n 5). Isolated MCAs mounted on two...