A5000354230- Neuropeptides and Animal Physiology
- Parkinson's Disease Mechanisms and Treatments
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Gastrointestinal motility and disorders
- Neuroscience and Neuropharmacology Research
- Zebrafish Biomedical Research Applications
- Retinal Development and Disorders
- Nerve injury and regeneration
- Regulation of Appetite and Obesity
- Peptidase Inhibition and Analysis
- Conducting polymers and applications
- Chemical synthesis and alkaloids
- Digestive system and related health
- Aquaculture disease management and microbiota
- Cephalopods and Marine Biology
- Infant Nutrition and Health
- Intestinal Malrotation and Obstruction Disorders
- Antimicrobial Peptides and Activities
- Neuroscience of respiration and sleep
- Glycosylation and Glycoproteins Research
- Cholinesterase and Neurodegenerative Diseases
- Botulinum Toxin and Related Neurological Disorders
- Electrochemical sensors and biosensors
- Aquaculture Nutrition and Growth
- Circadian rhythm and melatonin
Sapienza University of Rome
2010-2024
Shiga University of Medical Science
2000
Background and Aims. Melatonin is a ubiquitous hormone produced not only by the pineal gland but also other organs tissues. It involved in regulation of several gastrointestinal functions. The main cells responsible for production release extrapineal melatonin are enterochromaffin (EC) that produce serotonin. They pathogenesis neuromotor disorders characterize functional pathophysiology inflammatory intestinal diseases. Our aim was immunohistochemical highlighting on biopsy samples normal...
Alpha-synuclein (α-syn) is a presynaptic neuronal protein and its structural alterations play an important role in the pathogenesis of neurodegenerative diseases, such as Parkinson's disease (PD). It has been originally described brain aggregated α-syn also found peripheral nerves including enteric nervous system (ENS) PD patients. ENS network neurons glia gut wall which controls gastrointestinal function independently from central system. Moreover, two types epithelial cells are crucial...
Acetylcholine, the first neurotransmitter to be identified in vertebrate frog, is widely distributed among animal kingdom. The presence of a large amount acetylcholine nervous system cephalopods well known from several biochemical and physiological studies. However, little about precise distribution cholinergic structures due lack suitable histochemical technique for detecting acetylcholine. most reliable method visualize neurons immunohistochemical localization enzyme choline...
Alpha synuclein (α‐syn) is a 140 amino acid vertebrate‐specific protein, highly expressed in the human nervous system and abnormally accumulated Parkinson's disease other neurodegenerative disorders, known as synucleinopathies. The common occurrence of α‐syn aggregates suggested role for these although its biological activity remains poorly understood. Given high degree sequence similarity between vertebrate α‐syns, we investigated this proteins central (CNS) carp, Cyprinus carpio , with aim...
The α-, β- and γ-synucleins are small soluble proteins expressed in the nervous system of mammals evolutionary conserved vertebrates. After being discovered cartilaginous fish Torpedo californica, synucleins have been sequenced all vertebrates, showing differences number genes splicing isoforms different taxa. Although share high homology N-terminal sequence, suggesting their evolution from a common ancestor, three also differ molecular characteristics, expression levels tissue distribution....
The intestinal peptide guanylin regulates the electrolyte/water transport in gastrointestinal epithelium by paracrine/luminocrine mechanisms. Because also circulates blood, we investigated rat hypothalamo-pituitary region for expression and cellular localization of this peptide. Reverse transcriptase–PCR analyses with guanylin-specific primers revealed pars tuberalis distalis pituitary. Western blotting hypophyseal tissue extracts identified expected 12.5-kDa immunoreactive using two...
Cholinergic systems play a role in basic cerebral functions and its dysfunction is associated with deficit neurodegenerative disease. Mechanisms involved human brain diseases, are often approached by using fish models, especially cyprinids, given similarities of the to that mammals. In present paper, organization central cholinergic have been described cyprinid Cyprinus carpio, common carp, specific polyclonal antibodies against ChAT, synthetic enzyme acetylcholine, currently used as marker...
Galanin is a brain-gut peptide that present in the central and peripheral nervous systems. In gut, it contained exclusively intrinsic extrinsic nerve supplies, involved overall regulation of gut motility. To obtain information about ontogeny galanin, we undertook an immunohistochemical study chicken embryos. The time first appearance distribution patterns galanin were investigated with fluorescence streptavidin-biotin-peroxidase (ABC) protocols by using polyclonal antiserum. various regions...
Alpha-synuclein (α-syn) is widely expressed in presynaptic neuron terminals, and its structural alterations play an important role the pathogenesis of Parkinson's disease (PD). Aggregated α-syn has been found brain, peripheral nerves enteric nervous system (ENS) intestinal neuroendocrine cells during synucleinopathies inflammatory bowel disorders. In present study, we evaluated histomorphological features murine colon with 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced colitis, a common...
Cholangiocytes, the epithelial cells that line biliary tree, can proliferate under stimulation of several factors through both autocrine and paracrine pathways. The cocaine-amphetamine-regulated-transcript (CART) peptide has physiological functions, it is widely expressed in organs. CART increases survival hippocampal neurons by upregulating brain-derived neurotrophic factor (BDNF), whose expression been correlated to proliferation rate cholangiocytes. In present study, we aimed evaluate its...