A5052144059- Alzheimer's disease research and treatments
- Neuroscience and Neuropharmacology Research
- Cholinesterase and Neurodegenerative Diseases
- Nicotinic Acetylcholine Receptors Study
- Neuropeptides and Animal Physiology
- Cephalopods and Marine Biology
- Receptor Mechanisms and Signaling
- Neuroinflammation and Neurodegeneration Mechanisms
- RNA regulation and disease
- Parkinson's Disease Mechanisms and Treatments
- Chemical synthesis and alkaloids
- Bioinformatics and Genomic Networks
- Neurogenesis and neuroplasticity mechanisms
- Biosimilars and Bioanalytical Methods
- Neurobiology and Insect Physiology Research
- Gastrointestinal motility and disorders
- Memory and Neural Mechanisms
- Heart Failure Treatment and Management
- Neurological diseases and metabolism
- RNA Research and Splicing
- Neurotransmitter Receptor Influence on Behavior
- Ion Channels and Receptors
- S100 Proteins and Annexins
- RNA modifications and cancer
- Vagus Nerve Stimulation Research
Shiga University
2011-2025
Shiga University of Medical Science
2013-2025
Brigham and Women's Hospital
2023-2025
Harvard University
2023-2025
Inserm
1997-2001
Laboratoire de Neurobiologie Cellulaire et Moléculaire
2000
Mitochondrial ferritin (FtMt) is a type of that sequesters iron. Previous studies have shown FtMt expressed by dopaminergic neurons in the substantia nigra and it may be involved pathology Parkinson's disease. However, functional roles remain unclear. In this study, we investigated function α-synuclein regulation its antioxidant cells using human neuroblastoma cells, SH-SY5Y. physiological conditions, knockdown increased expression at protein level but not mRNA level. By contrast,...
Human genome studies have suggested that strawberry notch homologue 1 (SBNO1) is crucial for normal brain development, with mutations potentially contributing to neurodevelopmental disorders. In a previous study, we observed significant developmental abnormalities in the neocortex of Sbno1 as early one week after birth. present conducted an extensive analysis postnatal expression C57BL/6 mice using newly developed in-house polyclonal antibody against Sbno1. We found expressed all neurons,...
Abstract Progranulin (PGRN) is a protein encoded by the GRN gene with multiple identified functions including as neurotrophic factor, tumorigenic growth anti-inflammatory cytokine and regulator of lysosomal function. A single mutation in human resulting reduced PGRN expression causes types frontotemporal lobar degeneration dementia. Prosaposin (PSAP) also multifunctional neuroprotective secreted Interactions PSAP affect their functional properties. Their roles Alzheimer’s disease (AD),...
pChAT is a splice variant of peripheral type encoded alternatively by the gene for choline acetyltransferase common (cChAT), enzyme responsible acetylcholine synthesis. Immunohistochemistry using antiserum has successfully visualized many known cholinergic cells, whereas most cChAT antibodies failed to do so. As, however, accumulating evidence indicates that expression also occurs in various non-cholinergic neurons, we examined possible production rat dorsal root ganglion as model. The...
Summary: Purpose: To clarify the relationship of neuronal death to cellular responses, we studied as well reactions glia and progenitor cells in hippocampus two rat models epilepsy. Methods: Seizures were induced by either kainic acid (KA) administration or electrical kindling. Neuronal degeneration was assessed situ DNA fragmentation analysis. Reactions glial immunohistochemistry. Progenitor cell division evaluated using bromodeoxyuridine (BrdU) labeling method. Results: reactive microglia...
Decrease in multiple functions occurs the brain with aging, all of which can contribute to age-related cognitive and locomotor impairments. Brain atrophy specifically hippocampus, medial prefrontal cortex (mPFC), striatum, this age-associated decline function. Our recent metabolomics analysis showed changes these regions. To further understand aging processes, using a proteomics approach was carried out. This study conducted identify proteome profiles mPFC, striatum 14-, 18-, 23-,...
Acetylcholine acts as a neurotransmitter in the retina. Although previous physiological studies have indicated that some retinal ganglion cells may be cholinergic, several immunohistochemical using antibodies to choline acetyltransferase (ChAT) stained only amacrine but not cells. Recently, we identified splice variant of ChAT mRNA, lacking exons 6–9, rat peripheral nervous system. The encoded protein was designated type (pChAT), against which an antiserum raised. In present study, examined...
Mitochondrial ferritin (FtMt) is believed to play an antioxidant role via iron regulation, and FtMt gene mutation has been reported in age-related macular degeneration (AMD). However, little known about FtMt's functions the retina any links AMD. In this study, we observed increase hypoxia-inducible factor-1α (HIF-1α) murine retinal pigment epithelium (RPE). overexpression ARPE-19 cells stabilized HIF-1α, increased secretion of vascular endothelial growth factor. Conversely, HIF-1α...
Characterization of amyloid β (Aβ) oligomers, the transition species present prior to formation Aβ fibrils and that have cytotoxicity, has become one major topics in investigations Alzheimer's disease (AD) pathogenesis. However, studying pathophysiological properties oligomers is challenging due instability these protein complexes vitro. Here, we report conformation-restricted Aβ42 with an intramolecular disulfide bond at positions 17 28 (SS-Aβ42) formed stable Thioflavin T binding assays,...
The precision of colocalization analysis is enhanced by 3D and potentially more accurate than 2D. Even though improves the visualization analysis, rendering a model may generate with numerous polygons. We developed FtMt/LC3 followed simplification. Double immunofluorescence staining FtMt LC3 was conducted, stacked images were acquired. used IMARIS to render further processed it MeshLab decimate less complex model. examined available simplification algorithm using in detail evaluated...
Astrocytes expressing glutamic acid decarboxylase GAD67 directed by the glial fibrillary acidic protein promoter were shown to provide enhanced protection of PC12 cells from H 2 O treatment and serum deprivation in presence glutamate. In addition, they protected non‐differentiated, but not differentiated, embryonic rat cortical neurons glutamate toxicity. Glutamic (GAD)‐expressing astrocytes showed increased glutathione synthesis release compared control astrocytes. These changes due GAD...
We have recently discovered a splice variant of choline acetyltransferase (ChAT) mRNA and designated the protein pChAT because its preferential expression in peripheral neuronal structures. In this study, presence rat iris was examined by immunohistochemistry Western blot using antiserum, combination with RT-PCR analysis ChAT enzyme assay. For comparison, conventional (cChAT) studied parallel. By immunohistochemistry, intense labeling found to occur nerve fibers neurons ciliary trigeminal...
Cholinergic innervation of the heart has been analyzed using cholinergic markers including acetylcholinesterase, choline acetyltransferase (ChAT), and vesicular acetylcholine transporter (VAChT). In present study we demonstrate putative nerves in rat an antibody to ChAT a peripheral type (pChAT), which is product splice variant mRNA preferentially localized nerves. Expression mRNAs for pChAT conventional form (cChAT) were verified atrium by RT-PCR. Localization both protein products was...
Choline acetyltransferase (ChAT) synthesizes the neurotransmitter, acetylcholine, at cholinergic nerve terminals. ChAT contains nuclear localization signals and is also localized in nuclei of neural non-neuronal cells. Nuclear might have an as yet unidentified function, such transcriptional regulation. In this study, we investigated alteration candidate gene transcription by ChAT. We chose high affinity choline transporter (CHT1) vesicular acetylcholine (VACHT) genes, which function together...
Highlights•A novel antibody against human FtMt was characterized.•No cross-reaction of this with FTH observed.•FtMt distribution mapped in the brainstem.•FtMt co-localized catecholaminergic neurons VTA, SNc, and LC.•FtMt pattern suggested its function Parkinsonism.AbstractMitochondrial ferritin (FtMt), a recently-studied iron storage protein, which we suspect is an important defense oxidative stress elsewhere. The 242-amino acid precursor protein cleaved to mature (of molecular weight about...
We previously showed that degenerating adult motor neurons of the murine mutant wobbler, a model spinal muscular atrophy, express Transforming Growth Factor α (TGFα), growth factor endowed with glio- and neurotrophic activities. Here, we evaluated whether TGFα expression is general response to injury. Synthesis its precursor (pro-TGFα) was investigated in another motoneuronal degeneration, muscle deficient, hypoglossal following axonal crush cut. In control conditions, were devoid pro-TGFα...
Abstract Background We previously reported the specificity of a novel anti‐human peripheral choline acetyltransferase (hpChAT) antiserum for immunostaining cholinergic neuronal cell bodies and fibers in human colon. In this study, we investigate 3D architecture intrinsic innervation sigmoid colon relationship with nitrergic neurons enteric plexus. Methods developed modified CLARITY tissue technique applicable clearing specimens hpChAT co‐labeling nitric oxide synthase (nNOS) antibody. The...
Epidemiological studies suggest that poor nutrition during pregnancy predisposes offspring to the development of lifestyle-related noncommunicable diseases and psychiatric disorders later in life. However, molecular mechanisms underlying this predisposition are not well understood. In our previous study, using rats as model animals, we showed behavioral impairments induced by prenatal undernutrition. identified solute carrier 22 family member 23 (Slc22a23) a gene is irreversibly upregulated...
Abstract Choline acetyltransferase of the peripheral type (pChAT) is a splice variant that lacks exons 6–9 common‐type ChAT (cChAT); role pChAT remains unknown. We investigated expression and cChAT after axotomy to try elucidate its function. In dorsal motor nucleus vagus nerve (DMNV), ambiguus (NA), hypoglossal (HN) control rats, we observed neural but no pChAT‐positive neurons. Following transection, clearly detected pChAT‐labeled neurons in DMNV weakly labeled NA, was not seen HN. DMNV,...