Mitochondrial ferritin (FtMt) is a type of that sequesters iron. Previous studies have shown FtMt expressed by dopaminergic neurons in the substantia nigra and it may be involved pathology Parkinson's disease. However, functional roles remain unclear. In this study, we investigated function α-synuclein regulation its antioxidant cells using human neuroblastoma cells, SH-SY5Y. physiological conditions, knockdown increased expression at protein level but not mRNA level. By contrast,...

In cancer treatment, efficient delivery of active anticancer drugs into cells is highly desirable for maximizing therapeutic effects and alleviating side effects. this work, a nanocarrier consisting an Fe3O4 core, polyglycerol coating, octalysine functionality (SPION-PG-Lys8) has been designed, synthesized used to deliver photosensitizer, chlorin e6 (Ce6), photodynamic therapy (PDT) cells. SPION-PG-Lys8 colloidally stable in various aqueous solutions, showing high positive zeta potential...

Mitochondrial ferritin (MtF) has been identified as a novel encoded by an intron-lacking gene with specific mitochondrial localization located on chromosome 5q23.1. MtF associated neurodegenerative disorders such Friedreich ataxia and restless leg syndrome. However, little information is available about in Alzheimer's disease (AD). In this study, therefore, we investigated the expression of messenger RNA (mRNA) cerebral cortex AD control cases using real-time polymerase chain reaction (PCR)...

Studies have shown an increased expression of mitochondrial ferritin (FtMt) and antioxidant role for the protein in brains Alzheimer's disease (AD) patients. However, little information is available concerning FtMt other AD p

The human leucine-rich repeats and immunoglobulin-like domains (LRIG) gene family contains LRIG1, 2 3, encoding integral membrane proteins with an ectodomain, a transmembrane domain cytoplasmic tail. LRIG1 negatively regulates multiple receptor tyrosine kinases signaling including the epidermal growth factor (EGFR) is proposed tumor suppressor. soluble ectodomain demonstrated to be shed naturally inhibit progression of glioma. However, little known regarding functions LRIG2. In...

Mitochondrial ferritin (FtMt) is believed to play an antioxidant role via iron regulation, and FtMt gene mutation has been reported in age-related macular degeneration (AMD). However, little known about FtMt's functions the retina any links AMD. In this study, we observed increase hypoxia-inducible factor-1α (HIF-1α) murine retinal pigment epithelium (RPE). overexpression ARPE-19 cells stabilized HIF-1α, increased secretion of vascular endothelial growth factor. Conversely, HIF-1α...

Objective: We aim at investigating the molecular mechanisms through which Qingfei Jiedu decoction (QFJDD) regulates PD-L1 expression in lung adenocarcinoma (LUAD). Methods: Bioactive compounds and targets of QFJDD were screened from TCMSP, BATMAN-TCM, literature. Then, GeneCard, OMIM, PharmGKB, Therapeutic Target, DrugBank databases used to identify LUAD-related genes. The protein-protein interaction (PPI) network was constructed using overlapping bioactive LUAD with Cytoscape software...

Highlights•A novel antibody against human FtMt was characterized.•No cross-reaction of this with FTH observed.•FtMt distribution mapped in the brainstem.•FtMt co-localized catecholaminergic neurons VTA, SNc, and LC.•FtMt pattern suggested its function Parkinsonism.AbstractMitochondrial ferritin (FtMt), a recently-studied iron storage protein, which we suspect is an important defense oxidative stress elsewhere. The 242-amino acid precursor protein cleaved to mature (of molecular weight about...

Low intensity of Schistosoma infection is the current status in China after long time treatment with praziquantel, therefore more sensitive diagnostic methods are required now. In this study, a magnetic affinity enzyme-linked immunoassay (MEIA) based on signal transduction protein 14-3-3 japonicum (Sj14-3-3), was developed for detecting schistosomiasis.Sera infected BALB/c mice were collected and analyzed MEIA ELISA. Both ELISA Sj14-3-3 further used to detect serum IgG patients. Sera from 58...

Previous studies on the management of chronic subdural hematoma (cSDH) mainly focused risk postoperative recurrence and measures to prevent it. In this study, we propose use a non-invasive treatment method, modified Valsalva maneuver (MVM), as means reducing cSDH. This study aims clarify effects MVM functional outcomes rates.A prospective was conducted at Department Neurosurgery, Tongji Hospital, Medical College, Huazhong University Science Technology from November 2016 December 2020. The...

Mitochondrial ferritin (FtMt) is a novel iron storage protein with high homology to H-ferritin. Unlike the ubiquitously expressed H- and L-ferritin, FtMt in specific tissues such as testis, heart, brain. The function of not fully understood; however, evidence suggests that it has neuroprotective role neurodegenerative diseases. We have previously reported catecholaminergic neurons monkey brainstem. To explore expression human dopaminergic neurons, we designed monoclonal antibody, C65-2,...

ARNTL2 is a core component of the circadian clock genes and plays regulatory roles in cell cycle immune infiltration, but its mechanism lung cancer (LC) remains unclear. To investigate clinical therapeutic value LC. The Oncomine Cancer Cell Line Encyclopedia (CCLE) databases were adopted for assessing expression, after which Kaplan-Meier plotter Gene Expression Profiling Interactive Analysis 2 (GEPIA2) used to assess correlation with prognosis. univariate multivariate Cox regression analyses...

To observe the role of myeloperoxidase(MPO)and eosinophilic cationic protein(ECP)in airway inflammation and their correlation with clinical feature in asthma-COPD overlap (ACO) patients.Twenty patients COPD, 20 asthma, ACO control subjects underwent pulmonary function test for measurement forced expiratory volume 1 second (FEV1), vital capacity (FVC), peak flow (PEF), maximum midexpiratory (MMF25/75). COPD assessment (CAT) was used to evaluate symptoms ACO. The asthma (ACT) Induced sputum...

e21034 Background: In this retrospective study, we determined the differential expression of EGFR mutations between primary tumors and corresponding mediastinal nodal metastases, different lymph node stations in postoperative stage N2 non-small cell lung cancer (NSCLC). Methods: Results: all 28 cases with disease which had tumors, were detected 33 70 metastatic (47.14%) 17 (60.71%) who nodes. Among cases, 15 nodes, while 2 N1 Thirteen (46.43%) showed discordance etastases. 21 at least two 7...

Objective To explore the effect of overexpression LRIG3 gene on proliferation glioma cells and expression proliferating cell nuclear antigen (PCNA) Ki-67, possible mechanisms. Methods The plasmid containing control was transduced into U251 U87 respectively. mRNA protein levels were detected by using reverse transcription-polymerase chain reaction (RT-PCR) Western blotting. Cell examined methyl thiazol tetrazolium (MTT) assay. PCNA Ki-67 strept avidinbiotin complex (SABC). Results Compared...