A5101815723- 3D Printing in Biomedical Research
- Bone Tissue Engineering Materials
- Additive Manufacturing and 3D Printing Technologies
- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Osteoarthritis Treatment and Mechanisms
- Orthopaedic implants and arthroplasty
- Liver physiology and pathology
- Dental Implant Techniques and Outcomes
- DNA and Biological Computing
- Advanced biosensing and bioanalysis techniques
- Cell Image Analysis Techniques
- Periodontal Regeneration and Treatments
- Bone fractures and treatments
- Anatomy and Medical Technology
- Graphene and Nanomaterials Applications
- biodegradable polymer synthesis and properties
- Mesenchymal stem cell research
- Pluripotent Stem Cells Research
- Cancer Cells and Metastasis
- Modular Robots and Swarm Intelligence
- Cellular Mechanics and Interactions
- Iterative Learning Control Systems
- Manufacturing Process and Optimization
Tsinghua University
2013-2025
Ningxia Medical University
2024
Shanghai Urban Construction Design and Research Institute (Group)
2022
Materials Processing (United States)
2008
University of Newcastle Australia
2004
Using rapid prototyping technology, three-dimensional (3D) structures composed of hepatocytes and gelatin hydrogel have been formed. This technique employs a highly accurate 3D micropositioning system with pressure-controlled syringe to deposit cell/biomaterial lateral resolution 10 µm. The pressure-activated micro-syringe is equipped fine-bore exit needle for which wide variety patterns different arrays channels (through-holes) were created. More than 30 layers hepatocyte/gelatin mixture...
Creating functional tissues and organs in vitro on demand is a major goal biofabrication, but the ability to replicate external geometry of specific their internal structures such as blood vessels simultaneously remains one greatest impediments. Here, this limitation addressed by developing generalizable bioprinting strategy sequential printing reversible ink template (SPIRIT). It demonstrated that microgel-based biphasic (MB) bioink can be used both an excellent suspension medium supports...
Nerve guidance conduits (NGCs) have become a promising alternative for peripheral nerve regeneration; however, the outcome of regeneration and functional recovery is greatly affected by physical, chemical, electrical properties NGCs. In this study, conductive multiscale filled NGC (MF-NGC) consisting electrospun poly(lactide-co-caprolactone) (PCL)/collagen nanofibers as sheath, reduced graphene oxide /PCL microfibers backbone, PCL internal structure developed. The printed MF-NGCs presented...
Abstract Cancer models play critical roles in basic cancer research and precision medicine. However, current vitro are limited by their inability to mimic the three-dimensional architecture heterogeneous tumor microenvironments (TME) of vivo tumors. Here, we develop an innovative patient-specific lung assembloid (LCA) model using droplet microfluidic technology based on a microinjection strategy. This method enables precise manipulation clinical microsamples rapid generation LCAs with good...
Assembling multitype cells and biomimic extracellular matrix materials in a single construct is promising method to regenerate complex tissues/organs vitro. A new double-nozzle assembling technique was used fabricate hybrid cell/hydrogel with vascular-like network perform as mass exchange conduit. Using digital model mimic an anatomical liver structure, adipose-derived stromal (ADSC) were combined within gelatin/alginate/fibrinogen hydrogel form hepatocytes gelatin/alginate/chitosan placed...
There is a need for rapid fabrication of tissue or organs with well-defined structures and functions in regenerative medicine. Two patterns cell/matrix constructs containing hepatic cells, gelatin fibrinogen were successfully created by automated prototyping techniques stabilized thrombin. No apparent cell damage was found during the process. Mechanical characterization demonstrated that 1:1 ratio gelatin/fibrin mixture had greatest elasticity modulus compressive strength. Microscopic...
The rapid prototyping and manufacturing technology (RPM), is an integration of many different disciplines. It based on advanced dispersed-accumulated forming principle originated from 1980s. generates entity by first a series layers according to the dispersed section information digital model, then piling formed sequentially together. capable parts with complicated structures non-homogeneous materials. Traditional RPM techniques are mainly used as prototypes in product invention process,...
Abstract A major challenge in 3D extrusion bioprinting is the limited number of bioink that fulfills opposing requirements for printability with requisite rheological properties and functionality desirable microenvironment. Here, this limitation addressed by developing a generalizable strategy formulating cell‐laden microgel‐based biphasic (MB) bioink. The MB comprises two components, is, microgels close‐packed condition providing excellent bioprinting, hydrogel precursor forms second...
Three-dimensional (3D) bioprinting has emerged as a promising approach for engineering functional tissues and organs by layer-by-layer precise positioning of biological materials, living cells, biochemical components. Compared with nonbiological printing, 3D involves additional complexities technical challenges owing to the processing such appropriate biomaterials that fulfill requirements both printability functionality. In this review, we first introduce development course bioprinting,...
The formation of multiscale vascular networks is essential for the in vitro construction large-scale biomimetic cardiac tissues/organs. Although a variety bioprinting processes have been developed to achieve mesoscale and blood vessels, microvascular still mainly depends on self-assembly behavior endothelial cells (ECs), which inefficient demanding without appropriate stimulus. To address this problem, elongation connection engineered tissue (ECT) are sought promote by electrical stimulation...
An organ manufacturing technique was developed that enables the formation of cell/extracellular matrix (ECM) complexes for in vitro or vivo growth. In this study, a three-dimensional (3D) structure composed hepatocytes and gelatin/alginate hydrogel made using cell assembler-I apparatus to thoroughly control assembling. Hepatocytes ECM were constructed into 10 X 3mm 3 structures according designed pattern. The embedded remained viable performed biological functions construct more than 12...
Brain tissue engineering has now emerged as one of the most promising treatments for traumatic brain injury. In this article, two groups three-dimensional (3D) hydrogel structures composed gelatin and gelatin/hyaluronan have been formed using our 3D cell assembly technique in vivo study rats, order to investigate their effects reparation injury central nervous system (CNS). The were implanted into cortical defects created rat brains, abilities improve reconstruction then evaluated. After 4,...
The structure of an osteochondral biphasic scaffold is required to mimic native tissue, which owns a calcified layer associated with mechanical and separation function. two phases should possess efficient integration provide chondrocytes osteocytes independent living environment. In this study, novel composed bony phase, chondral phase compact was developed. layer-free taken as control group also fabricated. purpose current study evaluate the impact in scaffold. Bony were seeded autogeneic...
Integrative osteochondral repair is a useful strategy for cartilage-defect repair. To mimic the microenvironment, it necessary that scaffolds effectively extracellular matrix of natural cartilage and subchondral bone. In this study, biomimetic containing an oriented layer, compact three-dimensional (3D)-printed core-sheath structured-bone layer were developed. The was designed to structural material characteristics native tissue fabricated with matrix-chitosan materials, using...
Repairing osteochondral defect (OCD) using advanced biomaterials that structurally, biologically, and mechanically fulfill the criteria for stratified tissue regeneration remains a significant challenge researchers. Here, multilayered scaffold (MLS) with hierarchical organization heterogeneous composition is developed to mimic structure complex components of natural tissues. Specifically, intermediate compact interfacial layer within MLS designed resemble interface realize closely integrated...
Abstract Cancer stem cells (CSCs) are a rare cell population in tumors that responsible for tumor recurrence and metastasis. They priority as therapeutic targets, however, assays targeting CSCs have been limited by expanding maintaining vitro. Here, the authors find gelatin methacryloyl (GelMA)‐nanoclay hybrid hydrogels can induce enrich colorectal assisted three‐dimensional (3D) bioprinting. The presence of nanoclay increases printability, Young's modulus, pore size, cytocompatibility...
Abstract A hydroxyapatite/collagen (HAC) composite was produced to mimic the natural extracellular matrix of bone, with collagen serving as a template for apatite formation. three‐dimensional highly porous scaffold developed by mixing HAC poly( L ‐lactic acid) (PLA) using thermally induced phase separation technique. Naturally derived bovine bone morphogenetic protein (bBMP) incorporated into HAC‐PLA scaffolds, and then implanted in diaphyseal defects (2 cm radius) adult beagle dogs....