The purpose of this study was to investigate the role pore size on tissue ingrowth and neovascularization in porous bioceramics under accurate control parameters. For that purpose, β-tricalcium phosphate (β-TCP) cylinders with four different macropore sizes (300–400, 400–500, 500–600 600–700 µm) but same interconnection (120 unchangeable porosity were implanted into fascia lumbodorsalis rabbits. fibrous tissues blood vessels formed scaffolds observed histologically histomorphometrically....

A novel nanofibrous composite scaffold composed of super-paramagnetic γ-Fe2O3 nanoparticles (MNP), hydroxyapatite (nHA) and poly lactide acid (PLA) was prepared using electrospinning technique. The well responds extern static magnetic field with typical saturation magnetization value 0.049 emu/g as possesses architecture. scaffolds were implanted in white rabbit model lumbar transverse defects. Permanent magnets are fixed the cages to provide for rabbits post surgery. Results show that MNP...

Integrative osteochondral repair is a useful strategy for cartilage-defect repair. To mimic the microenvironment, it necessary that scaffolds effectively extracellular matrix of natural cartilage and subchondral bone. In this study, biomimetic containing an oriented layer, compact three-dimensional (3D)-printed core-sheath structured-bone layer were developed. The was designed to structural material characteristics native tissue fabricated with matrix-chitosan materials, using...

Abstract Three‐dimensional culture systems are an ideal in vitro model being capable of sustaining cell functionalities a manner that resembles the vivo conditions. In present study, we developed ultrasound trap‐based technique to rapidly produce HepG2 hepatocarcinoma aggregates within 30 min. Enhanced junctional F‐actin was observed at points cell–cell contact throughout aggregates. prepared by trap can be maintained on P‐HEMA‐coated surface for up 3 weeks. The cells these proliferated...

Long B, Dan L, Jian Yunyu H, Shu Zhi Y. Evaluation of a novel reconstituted bone xenograft using processed bovine cancellous in combination with purified morphogenetic protein. Xenotransplantation 2012; 19: 122–132. © 2012 John Wiley & Sons A/S. Abstract: Background: Xenogeneic grafting represents an alternative to autogenous osseous reconstruction and exhibits many beneficial properties. However, the usefulness xenogeneic relies on necessary processing procedures for removing antigens...

✓ A biomembrane was developed from pig peritoneum treated with 0.65% glutaraldehyde. This evaluated for use as a dural substitute in an animal model and patient population. After being the glutaraldehyde solution, lost its antigenicity while collagen underwent irreversible cross-linking reaction, causing it to become stable nonviable polymer resistant absorption by host. The used experimentally 43 procedures on 20 dogs applied clinically 614 patients. results demonstrated that is acceptable...

Three-dimensional highly porous poly(DL-lactic-co-glycolic acid)/ tricalcium phosphate (PLGA/TCP) scaffolds were fabricated using a rapid prototyping technique (RP). The 3D rhombic lamellar PLGA/TCP carriers (20 mm × 20 3 mm) subsequently coated with collagen type I (Col) to produce PLGA/TCP/Col composites. Both the RP-based and composites observed by scanning electron microscopy. Forty New Zealand white rabbits equally randomized into 2 groups (group A group B) bilaterally underwent...

The aim of the current study was to prepare microsphere-based composite scaffolds made nano-hydroxyapatite (nHA)/poly (DL-lactic-co-glycolic acid) (PLGA) at different ratios and evaluate effects nHA on characteristics for tissue engineering application. First, two nHA/PLGA (nHA/PLGA = 20/80 50/50) were prepared. Then, wettability, mechanical strength, degradation investigated. Second, biocompatibility osteoinductivity evaluated compared by co-culture with bone marrow stromal stem cells...

The minor alkaloids in tobacco play an important role the chemical composition of cigarette smoke, and they are precursors tobacco-specific nitrosamines (4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), N-nitrosonornicotine (NNN), N-nitrosoanabasine (NAB) N-nitrosoanatabine (NAT)). NNN NNK classified as group 1 carcinogens. A method quantitating both mainstream smoke has not been reported.Tobacco were extracted by sonication. extract was cleaned up dispersive solid-phase extraction,...

A method for simultaneous determination of acetaldehyde, propionaldehyde, acrolein, and crotonaldehyde in gas phase cigarette mainstream smoke by headspace chromatography-mass spectrometry was developed validated. Gas components were extracted with methanol, then the samples separated on a DB 624 (60 m, 0.32 mm x 1.8 mm) column, analyzed spectrometry, quantified isotope internal standard. The linearities good (R 2>0.992). recoveries between 78.5% 115%. relative standard deviations less than...

A simple spectrophotometric method was developed for the determination of trace formaldehyde in alcoholic beverage samples. The based on reaction between and chromotropic acid medium sulfuric aqueous hydrogen peroxide. monitored by measuring absorbance product at 575 nm after 30 min boil water bath. detection limit 0.033 mugldr mL-1 achieved. relative standard deviation 0.235 1.2 were 1.95% 0.93% (n = 5), respectively. found to be sensitive, selective applied with satisfactory results.

ABSTRACT Indirect determination of total monomelic and acid-reactive aluminum in natural waters by a.c. oscillopolarography the presence pyrocatechol violet (PCV) is presented this paper. In 0.5 mol 1−1 NH4Ac buffer solution (pH 6.4) containing l×lO−3 PCV, PCV yields two incisions on a.c oscillogram at potentials - 0.80 1.20 V, respectively. The incision depth declines when adding solution. decreases (Ep = -1.20 V) has a linear relationship with increasing Al concentrations (4×10−7 4×10−6...

In this paper, three different scale HA/PDLLA porous scaffolds, nano-HA/PDLLA, micro-HA/PDLLA and pure PDLLA were successfully fabricated using solvent casting/particulate leaching method. Chondrocytes adhesion proliferation on these scaffolds investigated. detail, the cells attachment rate quantitatively evaluated by cytometry. The interaction between chondrocytes observed optical microscope with HE staining FE-SEM. results exhibited that nano-HA/PDLLA scaffold has a modified cell property,...

Objective To explore the role of rapid prototyping (RP) in operation design for complicated fractures tibial plateau. Methods Included present study were 16 patients with complex fracture plateau who had undergone preoperative 3-D CT scanning before surgery from November 2006 to 2010.Their data transformed and imported into a RP machine pro duce models ratio 1: 1.The used classification by AO system design. Results According models,the was modified type 41B2.2 41B3.1 2 cases 41C2.1 toAO...

The effects of granulation methods,stem moisture contents and repetitive on stem waste,granulous yield filling power were studied by orthogonal experiments.The results showed that: 1) method significantly influenced power,the F values significance test 12.208,5.590,10.372 with the contribution rates 42.898%,53.410%,59.822%,respectively;2) also obviously affected granulous yield,the value rate 11.919 41.792%,respectively;3) stem's content these experimental as well,but not significant;4)...

A method for the simultaneous determination of 15 polycyclic aromatic hydrocarbons in cigarette filter was developed by isotope internal standard combined with gas chromatography-tandem mass spectrometry. The filters were extracted dichloromethane, and extract filtered 0.22 μm organic phase membrane. samples isolated DB-5MS column (30 m×0.25 mm, 0.25 μm) detected using multiple reaction monitoring mode electron impact source under positive ion mode. linearities (acenapthylene, acenaphthene,...