A5035059221- Bone Tissue Engineering Materials
- Bone fractures and treatments
- Periodontal Regeneration and Treatments
- Osteoarthritis Treatment and Mechanisms
- Graphene and Nanomaterials Applications
- Bone Metabolism and Diseases
- Orthopaedic implants and arthroplasty
- 3D Printing in Biomedical Research
- Hip and Femur Fractures
- Orthopedic Surgery and Rehabilitation
- Dental Implant Techniques and Outcomes
- Orthopedic Infections and Treatments
- Electrospun Nanofibers in Biomedical Applications
- Mesenchymal stem cell research
- Bone health and osteoporosis research
- Pelvic and Acetabular Injuries
- Nanoparticles: synthesis and applications
- Medical Imaging and Analysis
- Knee injuries and reconstruction techniques
- Tendon Structure and Treatment
- Reconstructive Surgery and Microvascular Techniques
- biodegradable polymer synthesis and properties
- Medical Imaging Techniques and Applications
- Neuropeptides and Animal Physiology
- Tissue Engineering and Regenerative Medicine
Air Force Medical University
2015-2024
Xijing Hospital
2015-2024
Central South University
2024
Chinese People's Liberation Army
2023
China XD Group (China)
2020-2021
People's Hospital of Bishan District
2011-2021
Committee on Publication Ethics
2021
Creative Commons
2021
Fuzhou General Hospital of Nanjing Military Command
2018
Wuhan No.1 Hospital
2015
Recent studies show that biomaterials are capable of regulating immune responses to induce a favorable osteogenic microenvironment and promote osteogenesis angiogenesis. In this study, we investigated the effects zinc silicate/nanohydroxyapatite/collagen (ZS/HA/Col) scaffolds on bone regeneration angiogenesis explored related mechanism. We demonstrate 10ZS/HA/Col significantly enhanced in vivo compared with HA/Col scaffolds. ZS/HA/Col increased tartrate-resistant acid phosphatase...
ABSTRACT Osteoporosis mainly affects postmenopausal women and older men. Gastrointestinal hormones released after meal ingestion, such as glucose-dependent insulinotropic peptide (GIP) glucagon-like (GLP)-2, have been shown to regulate bone turnover. However, whether GLP-1, another important gastrointestinal hormone, its analogues also antiosteoporotic effects, especially in aged situation, has not confirmed. In the present study, we evaluated effects of GLP-1 receptor agonist exendin-4 on...
Low-temperature additive manufacturing (AM) holds promise for fabrication of three-dimensional (3D) scaffolds containing bioactive molecules and/or drugs. Due to the strict technical limitations current approaches, few materials are suitable printing at low temperature. Here, a low-temperature robocasting method was employed print biomimic 3D bone regeneration using routine collagen–hydroxyapatite (CHA) composite material, which is too viscous be printed via normal methods The CHA had...
Glucagon-like peptide 1 (GLP-1) plays an important role in regulating bone remodeling, and GLP-1 receptor agonist shows a positive relationship with osteoblast activity. However, is not found osteoblast, the mechanism of on remodeling unclear. Here, we show that exendin-4 (Ex-4) promoted formation increased mass quality rat unloading-induced loss model. These functions were accompanied by increase number serum markers, while adipocyte was decreased. Furthermore, detected marrow stromal cells...
In recent years, graphene (G) and oxide (GO) nanoparticles have begun to be applied in surgical implant surface modification. However, biosafety antibacterial ability of G GO are still unclear. this study, the vitro was evaluated by co-culture with bone marrow mesenchymal stem cells (BMSCs) vivo observed implanting materials into mice muscle tissue. Biosafety results showed that 10 μg/ml safety critical concentration for GO. When more than μg/ml, cytotoxicity a dose-dependent manner....
Development of nano-laponite as bioinks based on cell-loaded hydrogels has recently attracted significant attention for promoting bone defect repairs and regeneration. However, the underlying mechanisms positive function laponite in hydrogel was not fully explored. In this study, effect 3D bioprinted construct regeneration potential mechanism explored vitro vivo. analyses showed that protected encapsulated cells from shear stresses during bioprinting, promoted cell growth spreading, BMSCs at...
Icariin had been reported as a potential agent for osteogenesis, but the dose-effect relationship needed further research to realize clinical application of icariin. We isolated and purified human bone mesenchymal stem cells (hBMSCs) stimulated them with different concentrations The cytotoxicity icariin was evaluated by methylthiazolytetrazolium (MTT) assay method. proliferation osteogenic differentiation such hBMSCs were investigated found that dose-dependent effect on in suitable...
As a newly emerging three-dimensional (3D) printing technology, low-temperature robocasting can be used to fabricate geometrically complex ceramic scaffolds at low temperatures. Here, we aimed 3D printed composed of nano-biphasic calcium phosphate (BCP), polyvinyl alcohol (PVA), and platelet-rich fibrin (PRF) temperature without the addition toxic chemicals.Corresponding nonprinted were prepared using freeze-drying method. Compared with scaffolds, had specific shapes well-connected internal...
Abstract The aim of this study was to investigate whether a surface coating with graphene could enhance the bioactivation titanium alloys (Ti 6 Al 4 V) further accelerate in vivo osteogenesis and osseointegration at implant surface. In study, New Zealand white rabbit femoral condyle defect model established. After 4, 12 24 weeks, biomechanical testing, micro-computed tomography (Micro-CT) analyses histological observations were performed. At highest push-out forces during test,...
Injectable hydrogels have long been gaining attention in the bone tissue engineering field owing to their ability mix homogeneously with cells and therapeutic agents, minimally invasive administration, seamless defect filling. Despite advantages, use of injectable as cell delivery carriers is currently limited by challenge mimicking natural microenvironment loaded cells, promoting proliferation, enhancing regeneration. To overcome these problems, we aimed develop an situ-forming...
Hydrogel is a suitable material for three-dimensional cell culture, and rich in water content can better simulate natural extracellular matrix. However, the relatively compact pores interior often inhibit growth differentiation of containing cells. Additionally, impact pore structure on cells remains to be unknown. In this work, porous hydrogel scaffolds with medium sizes (100–1000 μm) were prepared using 3D printing technology, without non-printed designed as control investigate effect...
The study is to investigate the chondrogenesis of a kind modified cell pellet formed using mesenchymal stem cells (MSCs) and gelatin microspheres containing transforming growth factor β3 (TGF-β3). loaded with TGF-β3 (MS-TGF) were prepared showed controlled release cytokine in biphasic fashion. Then mixture MSCs MS-TGF was centrifuged form pellet. cultured over 4 weeks determine effects on cartilage matrix production by biochemical analysis, immunohistochemistry staining, Western blot test....
Autologous platelet-rich plasma (PRP) has been extensively investigated for large bone defect treatment, but its clinical application is harassed by controversial outcome, due to highly variable PRP quality among patients. Alternatively, allogeneic from well-characterized donors cannot only generate more consistent and reliable therapeutic effect also avoid harvesting quantities of blood, an additional health burdens However, the use treatment generally less investigated, especially...
A Ti–6Al–4V alloy was modified with fibrinogen as mediated by polydopamine, and the skin-implant interface examined after transcutaneous implantation. The surface modification could inhibit epithelial downgrowth promote bone regeneration.
Our previous work showed that implanting a sensory nerve or vascular bundle when constructing vascularized and neurotized bone could promote osteogenesis in tissue engineering. This phenomenon be explained by the regulatory function of neuropeptides. Neuropeptide substance P (SP) has been demonstrated to contribute growth stimulating proliferation differentiation marrow stem cells (BMSCs). However, there have no prior studies on association between Wnt signaling mechanism SP context BMSC...
The mechanisms responsible for the phenomenon of an accelerated speed fracture healing in patients with traumatic brain injury (TBI) remain unclear. present study was performed to test hypothesis that TBI causes changes calcitonin gene-related peptide (CGRP) levels sera enhance healing. A standard closed femoral produced rats, which were subjected additional head trauma. assessed 4 and 8 weeks later using micro-CT. Sera, tissues muscles surrounding sites collected at 24, 48, 72 168 h after...
The objective of this study was to investigate whether surface coating with graphene could enhance the bioactivation PET-based artificial ligaments accelerate graft-to-bone healing after anterior cruciate ligament reconstruction. In an in vitro study, proliferation MC3T3-E1 cells and their differentiation on scaffolds were quantified via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide real-time polymerase chain reaction assays. significantly higher optical-density values...
Abstract Objectives Activation of the sympathetic system and adrenergic β‐receptors following traumatic bone defects negatively impairs regeneration. Whether preventing β‐receptor activation could potentially improve defect repair is unknown. In this study, we investigated effect systematic administration local delivery propranolol through composite scaffolds on healing. Materials methods Collagen/PVA/propranolol/hydroxyapatite（CPPH）composite were fabricated with 3D printing technique...
Background and objective: The modulus of carbon fiber-reinforced polyether ether ketone (CFR-PEEK), a composite containing layers fiber sheets, can be precisely controlled to match bone. However, CFR-PEEK is biologically inert cannot promote bone apposition. objective this study was investigate whether graphene modification could enhance the bioactivity CFR-PEEK. Methods results: In vitro, proliferation differentiation rat marrow stromal cells on scaffolds were quantified via cell-counting...