A5001161143- Pesticide Residue Analysis and Safety
- Mycotoxins in Agriculture and Food
- Insect and Pesticide Research
- Pharmaceutical and Antibiotic Environmental Impacts
- Pesticide and Herbicide Environmental Studies
- Plant and fungal interactions
- Wheat and Barley Genetics and Pathology
- Botanical Research and Chemistry
- Analytical Chemistry and Chromatography
- Bee Products Chemical Analysis
- Agriculture, Soil, Plant Science
- Microbial infections and disease research
- Antibiotics Pharmacokinetics and Efficacy
Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche
2021-2025
Mycotoxin contamination of feed and materials represent a serious health hazard. This study details the occurrence aflatoxin B1 (AFB1), zearalenone (ZEN) ochratoxin A (OTA) in 826 617 material samples, collected two Italian Regions (Umbria Marche) from 2018 to 2022 analyzed using UPLC-FLD platform. The developed method was validated accredited (ISO/IEC 17025) with satisfactory accuracy precision data obtained repeatability intralaboratory reproducibility conditions. Feed had higher incidence...
The presence of mycotoxins in cheese is a significant concern due to their potential health risks. Mycotoxins can contaminate through two main routes: indirectly via contaminated animal feed, and/or directly, because mold growth on dairy products. It has been reported that may contain metabolites aflatoxin B1 such as M1 (AFM1), aflatoxicol (AFL), and, its precursor, sterigmatocystin (STC). This study presents reliable method for the simultaneous determination AFM1, AFL, and STC cheeses made...
The collection of occurrence data on Alternaria toxins in food and feed across the European countries is required since 2012 by Commission, endorsing relevant scientific opinion EFSA CONTAM Panel. Within this framework, for (Alternariol, Alternariol monomethyl ether, Tenuazonic acid, Tentoxin, Altenuene) 97 samples cereal foods, tomato products, sunflower seeds have been provided as requested Italian national monitoring programme (years 2017-2020). To purpose, an LC-MS/MS method was set up...
The impact of soil fertilization with animal manure on the spread and persistence antibiotic resistance in environment is far from being fully understood. To add knowledge about correlations between residues genes (ARGs) fertilized soil, a longitudinal mesocosm study was conducted. Soil samples were collected mesocosms immediately before spreading then afterward at fifteen time points during 320-day observation period. Eight ARGs (ermB, sul1, tetA, tetG, tetM, cfr, fexA, optrA) class 1...
Alternaria toxins (ATs) are a group of produced by fungi that frequently contaminate tomatoes and tomato products. Recently, the European Food Safety Authority evaluated ATs for their genotoxic carcinogenic properties. infestation is often controlled using ad hoc treatment strategies (fungicides). In this study, two analytical methods were developed, validated applied determination five 195 pesticides in products collected from Italian market. Two distinct groups, organic (n = 20)...
The present manuscript reports on monitoring data of 12 ergot alkaloids (EAs) in cereal and cereal-derived products, collected Italy over the period 2017–2020, for official control purposes under edge Commission Recommendation 2012/154/EU presence EAs feed food. To these purposes, an LC-MS/MS method was set up applied, after in-house verification its analytical performance. Besides satisfactory recoveries precision, method’s quantification limits proved suitable to assess compliance cereals...
The detection and quantification of polar pesticides in liquid chromatography coupled with mass spectrometry present significant analytical challenges. This study compares the performance three LC columns (Hypercarb™, Raptor Polar X™, Anionic Pesticide™) separating quantifying eleven chicken eggs using a score-based methodology. Analytes include glyphosate, its metabolites, other high-polarity like Ethephon, Glufosinate, Fosetyl aluminum, included EU’s official control plan. pesticides,...
Aflatoxin M1 (AFM1) may be present in milk as a result of the ingestion aflatoxin B1 (AFB1), which could potentially contained dairy feed due to contamination by molds such Aspergillus flavus and A. parasiticus. AFM1 is toxic molecule classified IARC “potentially carcinogenic humans” Group 1. In addition four aflatoxins (AFB1, AFB2, AFG1, AFG2), these fungi also produce other toxins Sterigmatocystin (STC), while Aflatoxicol (AFL) metabolite AFB1. The aim this study was investigate transfer...
The impact of fertilization agricultural lands with animal manure on the spread and persistence antibiotic resistances in environment is still far from being understood. A longitudinal laboratory soil incubation study was conducted mimicking amendment practice using (slurry) taken a local swine farm. Soil samples were collected immediately before spreading and, after, at fifteen time points during 320 days period. Eight Antibiotic Resistance Genes, ARGs (ermBermB, sul1, tetA, tetG, tetM,...