Quality control and preprocessing of FASTQ files are essential to providing clean data for downstream analysis. Traditionally, a different tool is used each operation, such as quality control, adapter trimming filtering. These tools often insufficiently fast most developed using high-level programming languages (e.g. Python Java) provide limited multi-threading support. Reading loading multiple times also renders slow I/O inefficient.We fastp an ultra-fast preprocessor with useful...

Some applications, especially those clinical applications requiring high accuracy of sequencing data, usually have to face the troubles caused by unavoidable errors. Several tools been proposed profile quality, but few them can quantify or correct This unmet requirement motivated us develop AfterQC, a tool with functions errors and most them, plus highly automated quality control data filtering features. Different from tools, AfterQC analyses overlapping paired sequences for pair-end data....

Abstract Motivation Quality control and preprocessing of FASTQ files are essential to providing clean data for downstream analysis. Traditionally, a different tool is used each operation, such as quality control, adapter trimming, filtering. These tools often insufficiently fast most developed using high-level programming languages (e.g., Python Java) provide limited multi-threading support. Reading loading multiple times also renders slow I/O inefficient. Results We fastp an ultra-fast...

Removing duplicates might be considered as a well-resolved problem in next-generation sequencing (NGS) data processing domain. However, NGS technology gains more recognition clinical application, researchers start to pay attention its errors, and prefer remove these errors while performing deduplication operations. Recently, new called unique molecular identifier (UMI) has been developed better identify reads derived from different DNA fragments. Most existing duplicate removing tools cannot...

Solving a large-scale Poisson system is computationally expensive for most of the Eulerian fluid simulation applications. We propose novel machine learning-based approach to accelerate this process. At heart our deep convolutional neural network (CNN), with capability predicting solution (pressure) given discretization structure and intermediate velocities as input. Our consists four main components, namely, solve large linear equations, geometric describe spatial hierarchies input vector,...

Objective To evaluate the anesthetic effects and safety of ciprofol combined with low-dose alfentanil in sedation patients during Transesophageal Echocardiography (TEE).

Abstract Differential metabolites between tuberous roots from cultivated variety (ZP) and wild (YS) of Rehmannia glutinosa were analyzed by widely targeted metabolomics, annotated to KEGG pathways. 228 secondary (SM) in ZP YS detected, which 58 differential (DM), including 41 flavonoids, 10 phenolic acids, 3 terpenoids, 2 alkaloids others, 170 unchanged; Among DMs, 44 (75.9%) up-regulated YS, 30 unique while 14 (24.1%) down-regulated ZP; 33 (80.5%) more highly expressed than 7 (70%) 12 DMs...

Abstract DNA methylation plays an important role in the growth and development of plant, would change under different environments. In this study, 5-methyl cytosine (5mC) content level exhibited tissue specificity genomic wheat seedling, increased significantly leaf along with increase water deficit, which was especially significant AK58. Full-methylation might dominate full-methylation deficit higher than that hemi-methylation level. Under seedling showed polymorphism, polymorphism always...

Abstract In this study, ITS, ITS2, matK , rbcL and psbA-trnH in Rehmannia were successfully amplified sequenced, but some ITS sequences need to be proofread according ITS2 sequences. Compared with had higher mutation rate more information sites, interspecific diversity lower intraspecific variation the genetic of was lower. Furthermore, obvious barcoding gap found or + overlap between less. addition, phylogenetic tree based on indicated that R. glutinosa chingii henryi monophyly could...

A green and reliable method using supercritical fluid extraction (SFE) molecular distillation (MD) was optimized for the separation purification of standardized typical volatile components fraction (STVCF) from turmeric to solve shortage reference compounds in quality control (QC) components. high essential oil with 76.0% extracted by SFE. sequential strategy performed MD. The total recovery purity prepared STVCF were 97.3% 90.3%, respectively. Additionally, a strategy, i.e., STVCF-based...

Rehmannia glutinosa root contains many compounds with important medicinal properties and nutritional benefits, but only more than 140 have been reported so far. Many other their accumulation metabolic networks during its development remain unclear. In order to clarify them, profiles at three different developmental stages were analyzed using untargeted LC-MS analysis. Multivariate analysis revealed that 434 metabolites differently accumulated in stages, suggesting change trends. The having...

Highly conductive polypyrrole/graphene/manganese dioxide (PPy/graphene/MnO2) composites are fabricated via ultrasonic irradiation using p-toluenesulfonic acid as a dopant. The microstructures of PPy/graphene/MnO2 evidenced by the SEM and TEM examinations. effects graphene MnO2 loading on electrical conductivity investigated. maximum about 17.15 S/cm found with 3 wt% 48.7 at room temperature. Such uniform structure together observed high conductivities afforded specific capacitance. A...