A5014128484- Hereditary Neurological Disorders
- Signaling Pathways in Disease
- Multiple Sclerosis Research Studies
- Peripheral Neuropathies and Disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- melanin and skin pigmentation
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- Neurological diseases and metabolism
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Skin and Cellular Biology Research
- 3D Printing in Biomedical Research
- Cancer Cells and Metastasis
- Ion Transport and Channel Regulation
- Hair Growth and Disorders
- Molecular Biology Techniques and Applications
- Neurogenesis and neuroplasticity mechanisms
- Histone Deacetylase Inhibitors Research
- RNA Interference and Gene Delivery
- Wnt/β-catenin signaling in development and cancer
- Inflammasome and immune disorders
- Sirtuins and Resveratrol in Medicine
- Calcium signaling and nucleotide metabolism
- Ion channel regulation and function
Deggendorf Institute of Technology
2021-2025
Universitätsklinikum Würzburg
2016
University of Bergen
2011
University of Würzburg
2006-2010
Max Planck Society
2006
Max Planck Innovation
2006
Overexpression of the major myelin protein CNS, proteolipid (PLP), leads to late-onset degeneration and pathological changes in axons. Based on observation that white matter tracts these mutants both CD8+ T-lymphocytes CD11b+ macrophage-like cells are numerically elevated, we tested hypothesis pathologically involved primarily genetically caused neuropathy. Using flow cytometry mutant brains, could be identified as activated effector cells, confocal microscopy revealed a close association...
Abstract Malignant melanoma remains the most lethal form of skin cancer, exhibiting poor prognosis after forming distant metastasis. Owing to their potential tumor-suppressive properties by regulating oncogenes and tumor suppressor genes, microRNAs are important player in development progression. We defined loss miR-101-3p expression cells compared with melanocytes melanoblast-related as an early event aimed understand suppressive role its regulation cellular processes. Reexpression resulted...
The chemokine monocyte chemoattractant protein-1 (MCP-1/CCL2) has been previously shown to be an important mediator of macrophage-related neural damage in models two distinct inherited neuropathies, Charcot-Marie-Tooth (CMT) 1A and 1B. In mice deficient the gap junction protein connexin 32 (Cx32def), established model for X-chromosome-linked dominant form CMT (CMT1X), we investigated role macrophage immigration by crossbreeding Cx32def with MCP-1 knockout mutants. mutants typically...
Molecular analyses of normal and diseased cells give insight into changes in gene expression help understanding the background pathophysiological processes. Years after cDNA microarrays were established research, RNA sequencing (RNA-seq) became a key method quantitatively measuring transcriptome. In this study, we compared detection genes by each transcriptome analysis methods: array, quantitative RT-PCR, RNA-seq. As expected, found differences profiles aforementioned techniques. Here,...
Abstract Macrophages are critically involved in the pathogenesis of genetically caused demyelination, as it occurs inherited demyelinating neuropathies. On basis observation that upregulation Schwann cell‐derived chemokine MCP‐1 (CCL2) is a pathologically relevant mechanism for macrophage activation mice heterozygously deficient myelin component P0 (P0+/−), we posed question intracellular signaling cascade involved. By using western blot analysis peripheral nerve lysates MAP‐kinases...
Abstract Oncogene-induced senescence (OIS) is an important process that suppresses tumor development, but the molecular mechanisms of OIS are still under investigation. It known BRAF V600E -mutated melanocytes can overcome and develop melanoma, underlying mechanism largely unknown. Using established model primary transduced with , YAP activity was shown to be induced in as well melanoma cells compared normal epidermal melanocytes. This led assumption activation itself not a factor involved...
Progressive forms of multiple sclerosis lead to chronic disability, substantial decline in quality life and reduced longevity. It is often suggested that they occur independently inflammation. Here we investigated the disease progression mouse models carrying PLP1 point mutations previously found patients displaying clinical features sclerosis. These show loss-of-function associated with neuroinflammation; latter leading clinically relevant axonal degeneration, neuronal loss brain atrophy as...
Modifications in nuclear structures of cells are implicated several diseases including cancer. They result changes activity, structural dynamics and cell signalling. However, the role lamina related proteins malignant melanoma is still unknown. Its molecular characterisation might lead to a deeper understanding development new therapy approaches. In this study, we analysed functional effects dysregulated lamin B1 (LMNB1) its receptor (LBR). According their cellular localisation function,...
The bulge of hair follicles harbors Nestin+ (neural crest like) stem cells, which exhibit the potential to generate various cell types including melanocytes. In this study, we aimed determine role Sox9, an important regulator during neural development, in melanocytic differentiation those adult cells. Immunohistochemical analysis after conditional Sox9 deletion cells mice revealed that is crucial for these and acts as a fate determinant between glial fate. A deeper understanding factors...
Alginate hydrogels have been used as a biomaterial for 3D culturing several years. Here, gene expression patterns in melanoma cells cultivated alginate are compared to 2D cultures. It is well-known that cell culture not resembling the complex vivo situation well. However, use of very intricate models does allow performing high-throughput screening and analysis highly complex. strategies will better mimic while they maintain feasibility large-scale analysis. As an easy-to-use material due its...
The tumor suppressive role of CYLD lysine 63 deubiquitinase (CYLD) is known in melanoma. To the best our knowledge, however, precise mechanism underlying function has yet to be clarified. In present study, a novel melanoma mouse model was generated, which revealed accelerated growth Cyld‑knockout (Cyld‑/‑) compared with Cyld‑wild‑type (Cyld+/+) mice. determine molecular mechanism, mutation analysis primary tumor‑derived cell lines from Cyld+/+ and Cyld‑/‑ mice performed using RNA sequencing...
In malignant melanoma, a highly aggressive form of skin cancer, many microRNAs are aberrantly expressed contributing to tumorigenesis and progression. Further, deregulation microRNA processing enzymes, like the miRNA-binding protein Argonaute 2, significantly impacts function. This study characterizes novel splice variant Argonaut AGO2-ex1/3. AGO2-ex1/3 is substantially in different melanoma cell lines patient-derived tissue samples. It mature mRNA, which translated into an N-terminally...
The skin of adult mammals protects from radiation, physical and chemical insults. While melanocytes melanocyte-producing stem cells contribute to proper function in healthy organisms, dysfunction these can lead the generation malignant melanoma-the deadliest type cancer. Addressing melanocyte lineage vivo represents a prerequisite for understanding melanoma on cellular level development preventive treatment strategies. Here, inducible Cre-loxP-system has emerged as promising tool...