In this study, the effect of tilapia gelatin peptides (TGP) on UV-induced damages to mice skin was evaluated. The antioxidant indicators, lipid, collagen and glycosaminoglycan were determined histological changes depicted. results showed TGP could alleviate abnormal protective in dose-dependent manners. protect lipid from UV radiation damages, change also recovered significantly. action mechanisms mainly involved properties repairing endogenous synthesis. Therefore, key peptide further...

The goal of this work was to normalize striatal tyrosine hydroxylase (TH) activity with intravenous nonviral TH gene therapy and at the same time eliminate ectopic expression in peripheral organs such as liver rat. TH-expression plasmids, containing either SV40 promoter or glial fibrillary acidic protein (GFAP) promoter, were globally delivered brain across blood-brain barrier (BBB) after administration pegylated immunoliposomes (PILs). GFAP-TH- SV40-TH-expression plasmids encapsulated...

Antihypertensive effect of long-term oral administration jellyfish (Rhopilema esculentum) collagen peptides (JCP) on renovascular hypertension rats (RVHs) was evaluated. The systolic blood pressure and diastolic the RVHs were significantly reduced with JCP (p < 0.05), compared model control group. However, arterial normal showed no significant changes during treatment high dose > 0.05). Furthermore, angiotensin II (Ang II) concentration plasma had significance but inhibited Ang in...

Rapid urbanization and intensification of human activities increases the risk disturbance ecological systems via multiple sources, with consequences for regional security health. Landscape assessment (LERA) is an effective way to identify allocate resources. We used north south Qinling Mountain area as a case study analyze spatial heterogeneity landscape using potential- connectedness-resilience three-dimensional (PCR 3D) framework based on integrated dynamic concept from adaptive cycle...

Abstract Glial‐derived neurotrophic factor (GDNF) is a neurotrophin that could be developed as neurotherapeutic for Parkinson's disease, stroke, and motor neuron disease. However, GDNF does not cross the blood–brain barrier (BBB). Human was re‐engineered by fusion of mature protein to carboxyl terminus chimeric monoclonal antibody (MAb) human insulin receptor (HIR). The HIRMAb‐GDNF bi‐functional, both binds HIR, trigger receptor‐mediated transport across BBB, (GFR)‐α1, activate...

Abstract Neurotrophins, such as brain derived neurotrophic factor (BDNF), do not cross the blood–brain barrier (BBB). Certain monoclonal antibodies (MAb) to human insulin receptor (HIR) BBB via receptor‐mediated transport, and can act a molecular Trojan horse ferry across an attached drug. A genetically engineered fusion protein was produced whereby amino terminus of BDNF is fused carboxyl heavy chain chimeric HIRMAb. The HIRMAb‐BDNF reacted equally with IgG BDNF. bi‐functionality retained...

Coconut cake is an abundant and good potential edible protein source. However, until now it has not been extensively used in the food industry. To promote its usage, characterization, nutrition value antioxidant activity of coconut fractions (albumin, globulin, prolamine, glutelin-1 glutelin-2) were studied. Results revealed that all albumin, glutelin-2 showed a high value. The exhibited radical scavenging reducing power, globulin prolamine ion chelating ability (89.14-80.38%). Moreover,...

The differences in chemical components, nutritional value, volatile organic compounds, antioxidant activity and α-glucosidase inhibiting capacity vitro coconut waters with different maturities (8, 10, 12 months after pollination germination height below 10 cm were named CW-8, CW-10, CW-2, MCW, respectively) from the tall variety compared analyzed. Results showed that as maturity increased, ash reducing sugar water gradually decreased, while protein content fatty acids continued to increase....

Sargassum maclurei is a potential protein resource because of its high content and relatively balanced amino acid composition. To promote usage in food, medical, or other industries, S. was hydrolyzed by pepsin papain to obtain bioactive peptides. The hydrolysates (SMPHs) were purified using gel chromatography reversed-phase performance liquid (RP-HPLC), 12 major fractions obtained. fraction D11 with the highest angiotensin I-converting enzyme (ACE) inhibition (61.59%, at 1 mg/mL) subjected...

In recent years, angiotensin-converting enzyme (ACE) inhibitory peptide has become a research hotspot because of its essential role in maintaining human blood pressure balance. this study, two novel ACE peptides Val-Glu-Arg-Gly-Arg-Arg-lle-Thr-Ser-Val (Valine-Glutamate-Arginine-Glycine-Arginine-Arginine-Isoleucine-Threonine-Serine-Valine, VERGRRITSV) and Phe-Val-Ile-Glu-Pro-Asn-Ile-Thr-Pro-Ala (Phenylalanine-Valine-Isoleucine-Glutamate-Proline-Asparagine-Isoleucine-Threonine-Proline-Alanine,...

The genetic engineering, expression, and validation of a fusion protein avidin (AV) chimeric monoclonal antibody (mAb) to the human insulin receptor (HIR) is described. 15 kDa monomer was fused carboxyl terminus heavy chain HIRMAb. reacted with antibodies specific for IgG avidin, had same affinity binding HIR extracellular domain as original qualitatively bound biotinylated ligands, but secreted fully saturated biotin by COS cells, owing high level in tissue culture medium. Chinese hamster...

In order to promote the development and utilization of coconut haustorium (CH). The basic chemical composition, volatile profiles antioxidant activities three haustoria with different transverse diameters were investigated. Results showed large (LCH) contained more soluble sugar (47.10%) reducing (17.68%), while small (SCH) possessed ash (10.17%), protein (9.22%) fat (5.03%). All CH rich in potassium (4.06-4.69%) phosphorus (0.39-0.50%). fatty acid composition SCH amino middle (MCH) was...

The effects of the dry processing coconut oil on amino acid composition, molecular weight, secondary structure, solubility, surface hydrophobicity, microstructure, total sulfhydryl and free content, thermal properties, water-holding, oil-holding, foaming, emulsifying properties isolate protein were investigated. results showed that altered composition proteins as well resulted in fewer irregular structural regions more homogeneous particle sizes, leading to an improvement stability proteins....

Two genes encoding putative glutathione S-transferase proteins were isolated from pear (Pyrus pyrifolia) and designated PpGST1 PpGST2. The deduced PpGST2 contain conserved Glutathione N-terminal domain (GST_N) S-transferase, C-terminal (GST_C). Using PCR amplification technique, the genomic clones corresponding to shown two introns a singal intron respectively with typical GT/AG boundaries defining splice junctions. Phylogenetic analysis clearly demonstrated that belonged Phi class of GST...

Significance Lineage analysis is an important assay for developmental biology, cancer etc. Traditional tools in this field are time consuming, technically challenging, and demand of preexisting knowledge. By integrating exogenous barcodes into cells, single-cell RNA-sequencing (scRNA-seq) can be used to conduct such tasks, but these assays required significant expertise both wet- dry-laboratory experiments. We developed a user-friendly algorithm cell-lineage inference solely based on...

This paper reports the purification, characterization,<italic>in vivo</italic>and<italic>in vitro</italic>antihypertensive activity of two novel peptides derived from coconut cake globulin hydrolysates.

Four antioxidant peptides were identified from enzymatic hydrolysates of palm kernel expeller glutelin-1 in the current study.

Summary Extraction, physicochemical properties and fatty acid composition analysis of coconut testa oil (CTO), antioxidant activity the protective effect on oxidative damage to human serum albumin (HSA) extract (CTOE) were investigated. Results showed that optimal condition CTO was B 3 A 2 C (temperature 60 °C, material‐to‐solvent 1:4 g mL −1 extraction time h) with maximum yield (76.83 ± 0.53%). The obtained nondrying iodine value 14.69 per 100 g, lauric main component 42.28%. Hydrogen...

This paper provides valuable information on the nutritional quality, functional properties, antioxidative and ACE inhibition of palm kernel expeller protein fractions.

Glutelin-1 of defatted walnut meal protein (DWPG-1) was modified by pepsin enzymatic hydrolysis to improve its functional properties and antioxidant activities. The amino acid composition, structural characteristics, physicochemical as well activities the hydrolysate were compared with those unmodified DWPG-1. analysis X-ray diffraction patterns, surface microstructure particle size distribution indicated that changed structures Compared natural unhydrolyzed protein, showed better...

In this study, the changes in conventional nutrient and mineral compositions as well metabolomics characteristics of red palm weevil (RPW)