A5059312822- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Reproductive Biology and Fertility
- Muscle Physiology and Disorders
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Genetics and Neurodevelopmental Disorders
- Speech Recognition and Synthesis
- RNA Research and Splicing
- Speech and Audio Processing
- Genomics and Phylogenetic Studies
- Organic Light-Emitting Diodes Research
- Photocathodes and Microchannel Plates
- Advanced Proteomics Techniques and Applications
- Phonetics and Phonology Research
- RNA regulation and disease
- GaN-based semiconductor devices and materials
- Aldose Reductase and Taurine
- Renal and related cancers
- Congenital heart defects research
- Ga2O3 and related materials
- Peroxisome Proliferator-Activated Receptors
- Animal Genetics and Reproduction
- Phase-change materials and chalcogenides
- Protein Structure and Dynamics
The University of Tokyo
2012-2022
Keio University
2003-2020
Keio University Hospital
2017
University of Florida
2012
National Agriculture and Food Research Organization
2012
Abstract Background The first distinct differentiation event in mammals occurs at the blastocyst stage when totipotent blastomeres differentiate into either pluripotent inner cell mass (ICM) or multipotent trophectoderm (TE). Here we determined, for time, global gene expression patterns ICM and TE isolated from bovine blastocysts. were blastocysts harvested day 8 after insemination by magnetic activated sorting, cDNA sequenced using SOLiD 4.0 system. Results A total of 870 genes...
Efficient differentiation of human pluripotent stem cells (hPSCs) into neurons is paramount for disease modeling, drug screening, and cell transplantation therapy in regenerative medicine. In this manuscript, we report the capability five transcription factors (TFs) toward aim: NEUROG1, NEUROG2, NEUROG3, NEUROD1, NEUROD2. contrast to previous methods that have shortcomings their speed efficiency, a cocktail these TFs as synthetic mRNAs can differentiate hPSCs 7 days, judged by calcium...
Intrinsically disordered regions in proteins are known to evolve rapidly while maintaining their function. However, given lack of structure and sequence conservation, the means through which they stay functional is not clear. Poor conservation also hampers classification these into groups. We studied a large number predicted experimentally determined intrinsically from human proteome 7 other eukaryotes. chemical composition by calculating fraction positive, negative, polar, hydrophobic...
Harnessing epigenetic regulation is crucial for the efficient and proper differentiation of pluripotent stem cells (PSCs) into desired cell types. Histone H3 lysine 27 trimethylation (H3K27me3) functions as a barrier against through suppression developmental gene expression in PSCs. Here, we have generated human PSC (hPSC) lines which genome-wide reduction H3K27me3 can be induced by ectopic catalytic domain histone demethylase JMJD3 (called JMJD3c). We found that transient, forced...
Transcription factors (TFs) play a pivotal role in determining cell states, yet our understanding of the causative relationship between TFs and states is limited. Here, we systematically examine state changes human pluripotent embryonic stem cells (hESCs) by large-scale manipulation single TFs. We establish 2,135 hESC lines, representing three clones each 714 doxycycline (Dox)-inducible genes including 481 TFs, obtain 26,998 microscopic images 2,174 transcriptome datasets—RNA sequencing...
The exon junction complex (EJC) that is deposited onto spliced mRNAs upstream of exon–exon junctions plays important roles in multiple post-splicing gene expression events, such as mRNA export, surveillance, localization, and translation. However, a direct role for the human EJC pre-mRNA splicing has not been fully understood. Using HeLa cells, we depleted one core components, Y14, resulting transcriptome was analyzed by deep sequencing (RNA-Seq) confirmed RT–PCR. We found Y14 required...
Direct generation of skeletal muscle cells from human pluripotent stem (hPSCs) would be beneficial for drug testing, discovery, and disease modelling in vitro. Here we show a rapid robust method to induce myogenic differentiation hPSCs by introducing mRNA encoding MYOD1 together with siRNA-mediated knockdown POU5F1 (also known as OCT4 or OCT3/4). This integration-free approach generates functional myotubes sarcomere-like structure fusion capacity several days. The silencing facilitates...
Cell-type-specific genes exhibit heterogeneity in genomic contexts and may be subject to different epigenetic regulations through gene transcriptional processes depending on the cell type involved. The gene-body regions (GBRs) of some cardiomyocyte (CM)-specific are long highly hypomethylated CMs. To explore cell-type specificities patterns functions, multiple modifications GBRs were compared among CMs, liver cells embryonic stem (ESCs).We found that most show a moderately negative...
Taurine, a sulfur-containing β-amino acid, is present at high concentrations in mammalian tissues and plays an important role several essential biological processes. However, the genetic mechanisms involved these physiological processes associated with taurine remain unclear. In this study, we investigated regulatory mechanism underlying taurine-induced transcriptional enhancement of thioredoxin-interacting protein (TXNIP). The results showed that significantly increased luciferase activity...
The first distinct differentiation in mammals occurs at the blastocyst stage. Here we determined global gene expressions patterns inner cell mass (ICM) and trophectoderm (TE) isolated from bovine blastocysts using next generation sequencing evaluated how CSF2, which increases embryo competence to complete development term, alters expression. Bovine embryos were produced vitro treated with 0 or 10 ng/ml CSF2 Day 6 8 after insemination. Blastocysts harvested 8, ICM TE isolated, cDNA sequenced...
The variable bit rate PARCOR-VQ hybrid vocoder uses the PARCOR scheme for unvoiced or transient periods and VQ (vector quantization) voiced with a method. ratio of is much higher than that periods. Thus, it expected average proposed will be lower vocoder. With respect to accuracy analysis-synthesis (important recognition words), better because its It also reconstructed speech By computer simulation listening tests, was confirmed vocoder, whose 919 b/s, has nearly same quality as 2.4 kb/s...