Recombinant human deoxyribonuclease (rhDNase) has been shown to improve lung function and reduce the number of pulmonary exacerbations in patients with cystic fibrosis (CF), but its long-term effect on airway inflammation remains unknown. In this study, we used bronchoalveolar lavage (BAL) investigate rhDNase CF having mild disease. A total 105 (> or =5 years age) normal were randomized receive (2.5 mg/day) no rhDNase. Patients a percentage neutrophils BAL fluid at baseline not served as...

Summary. Continuous therapy with antistaphylococcal antibiotics is advocated by some cystic fibrosis (CF) centers, but it unclear whether this strategy favors early colonization P. aeruginosa. We used the data base for German Centers of European Registry Cystic Fibrosis (ERCF) to assess effect continuous antistaphyloccocal on rate aeruginosa acquisition in CF patients. Patients included analysis had be < 18 years age, aeruginosa-negative prior entry ERCF, and have at least 2 additional...

About 36.7 million people living with HIV are reported worldwide. In Eastern Africa, young women (aged 15–24 years) accounted for 26% of new infections in 2016 despite making up just 10%of the population. These include 2.7million children less than fifteen years age and 17.6 women. Hence, due to high prevalence rate children, preventing mother child transmission has become an essential element worldwide HIV/AIDS control strategy. The study was assess prevention services utilization among...

Recombinant DNase (dornase alpha) was shown to improve lung function and reduce pulmonary exacerbations in cystic fibrosis (CF) patients, but its effects on DNA concentrations the lower airways remain unclear at present time. As part of Bronchoalveolar Lavage Evaluation Anti-Inflammatory Treatment (BEAT) Study, a multicenter open study evaluate evolution inflammation CF patients with early disease modulation by dornase alpha treatment, we studied bronchoalveolar lavage (BAL) fluid 48 mild...

Cystic fibrosis (CF) lung disease is primarily a of the small airways. We hypothesized that even in patients with normal function, reduced surfactant function would be present and favor airway obstruction. Bronchoalveolar lavages from 76 CF (5-31 years, median 11) well-conserved (FEV1 94% predicted, range 78-121) 10 healthy control subjects were investigated. The deviation biophysical performance normal, assessed bubble surfactometer, was small; however, ability to maintain patency narrow (%...

Bronchoalveolar lavage (BAL) has been shown to be useful in the diagnosis of hypersensitivity pneumonitis (HP) adults, typical constellation being lymphocytosis with a decrease CD4/CD8 ratio. Only limited data exist for diagnostic value BAL cytology paediatric patients this disorder. Children aged 6–15 yrs (n=9) acute HP were studied. was performed before initiation anti­inflammatory treatment via flexible bronchoscope middle lobe 3 mL·kg body weight −1 normal saline warmed temperature; and...

A controversy exists concerning the adequate specimen to characterise colonisation of cystic fibrosis (CF) airways by Pseudomonas aeruginosa . Oropharyngeal, sputum and bronchoalveolar lavage samples were evaluated from 38 stable CF patients for detection P. , genetically different isolates within same host longitudinal variations in genotype during repeated examinations. Bacterial typed pulsed-field gel electrophoresis deoxyribonucleic acid macrorestriction fragments. Sensitivity, negative...

Lung inflammation plays a pivotal role in the pathogenesis of airway disease cystic fibrosis (CF). An imbalance between pro- and anti-inflammatory mediators has been observed deficiency response proposed, but this concept remains controversial. In present study, concentrations two mediators, lipoxin A (LxA4) Clara cell protein 10 (CC-10), were assessed bronchoalveolar lavage fluid (BALF) CF patients with wide range endobronchial controls neutrophilic unrelated to CF. No differences LxA4 BALF...

Cystic fibrosis (CF) is associated with a neutrophil dominated airway inflammation. So far bronchoalveolar lavage (BAL) studies in CF have used pooled BAL samples which may be more representative of the alveolar compartment rather than airways. To assess whether first sample sensitive evaluation inflammation, authors studied 105 stable patients aged 5-37 yrs mean forced expiratory volume one second (FEV1) 96+/-15% (mean+/-SD). cytology and were compared to reference values obtained children...

It has been suggested that atopy is associated with an impairment in the δ6‐desaturation of (n‐6)‐polyunsaturated fatty acids and subsequently low levels eicosanoid precursors. To evaluate this hypothesis we analyzed acid composition plasma phospholipids cholesterolest‐ers a well‐defined group children atopic bronchial asthma (n = 17) age‐matched healthy controls 10). Atopic showed significantly higher linoleic lower proportions arachidonic lipids. No differences were observed respect to...

Summary: Thirteen patients with myelomeningocele who required operation for scoliosis were tested changes in pulmonary function 1 month before and an average of 13 months after spinal stabilization. The age at the time was 12 years 11 months. Ten had restrictive preoperatively. Owing to severity scoliosis, anterior approach spine including resection ribs division diaphragm necessary eight children. Postoperatively, despite these procedures, increase vital capacity six increased forced...

Abstract Background In a cross-sectional analysis of cystic fibrosis (CF) patients with mild lung disease, reduced surfactant activity was correlated to increased neutrophilic airway inflammation, but not function. So far, longitudinal measurements function in CF are lacking and it remains unclear how these alterations relate the progression inflammation as well decline pulmonary over time. Methods As part BEAT trial, study assess course CF, we studied function, endobronchial using...

Abstract Background The surfactant proteins B (SP-B) and C (SP-C) are important for the stability function of alveolar film. Their involvement down-regulation in inflammatory processes has recently been proposed, but their level during neutrophilic human airway diseases not yet known. Methods We used 1D-electrophoresis Western blotting to determine concentrations molecular forms SP-B SP-C bronchoalveolar lavage (BAL) fluid children with different diseases. 21 cystic fibrosis, 15 chronic...

Neutrophil-dominated endobronchial inflammation is a major characteristic of cystic fibrosis (CF) and there increasing demand for easy-to-perform noninvasive monitoring prediction intervention. Fourteen stable paediatric CF patients (8–17 yrs; mean forced expiratory volume in one second 86.7% the predicted value) were investigated once by fractional bronchoalveolar lavage (BAL) sputum induction on three occasions, 2–6 weeks apart. Sputum was induced consecutive 10‐min inhalations 3, 4 5%...

Vocal cord dysfunction (VCD) is a paradoxical function of the vocal cords, leading to intermittent predominantly inspiratory dyspnea, but with no response bronchodilator and anti‐inflammatory drug therapy. We report on three children VCD: 1) A 12‐year old boy, who was treated for many years bronchial asthma presented dyspnea functional reduction expiratory flow‐volume curve, 2) 13‐year girl also long‐term basis in whom movement could be demonstrated by laryngoscopy, 3) 17‐year who, besides...