A5067526056- Advanced Proteomics Techniques and Applications
- Neonatal Respiratory Health Research
- Preterm Birth and Chorioamnionitis
- Pregnancy and preeclampsia studies
- Mass Spectrometry Techniques and Applications
- Peptidase Inhibition and Analysis
- Antibiotics Pharmacokinetics and Efficacy
- Monoclonal and Polyclonal Antibodies Research
- Redox biology and oxidative stress
- Central Venous Catheters and Hemodialysis
- Streptococcal Infections and Treatments
- Drug-Induced Adverse Reactions
- Nutrition, Genetics, and Disease
- Carcinogens and Genotoxicity Assessment
- Retinoids in leukemia and cellular processes
- Reproductive System and Pregnancy
- Microbial Metabolism and Applications
- Metabolism, Diabetes, and Cancer
- Anesthesia and Sedative Agents
- Biotin and Related Studies
- Neonatal and Maternal Infections
- Multiple Myeloma Research and Treatments
- Pregnancy and Medication Impact
- Lung Cancer Research Studies
- Infant Nutrition and Health
University Hospital Hradec Králové
2017-2025
Centre of Biomedical Research
2025
University of Defence
2012-2020
University of Hradec Králové
2017
Due to its sensitivity and productivity, bottom-up proteomics based on liquid chromatography–mass spectrometry (LC–MS) has become the core approach in field. The de facto standard LC–MS platform for operates at sub-μL/min flow rates, nanospray is required efficiently introducing peptides into a mass spectrometer. Although this almost "dogma", view being reconsidered light of developments highly efficient chromatographic columns, especially with introduction exceptionally sensitive MS...
Abstract Background Antimicrobial lock therapy is recommended for preventing and treating catheter‐related bloodstream infections, but different solutions have uncertain efficacy. Methods Two locks, 1.35% taurolidine 4% ethylenediaminetetraacetic acid (EDTA), were tested on Staphylococcus epidermidis , aureus methicillin‐resistant S. Pseudomonas aeruginosa multidrug‐resistant P. vancomycin‐resistant Enterococcus faecium Klebsiella oxytoca (carbapenemase producing), K. pneumoniae...
The efforts to utilize microflow liquid chromatography hyphenated tandem mass spectrometry (μLC-MS/MS) for deep-scale proteomic analysis are still growing. In this work, two-dimensional LC separation and peptide derivatization by a tag (TMT) were used assess the capability of μLC-MS/MS reveal protein changes associated with severe chronic anthracycline cardiotoxicity phenotype in comparison nanoflow (nLC-MS/MS). control anthracycline-treated rabbit myocardium nLC-MS/MS allowed quantification...
Cellular senescence is a complex stress response defined as an essentially irreversible cell cycle arrest mediated by the inhibition of cycle-specific cyclin dependent kinases. The imbalance in redox homeostasis and oxidative have been repeatedly observed one hallmarks senescent phenotype. However, large-scale study investigating protein oxidation signaling cells vitro has lacking. Here we applied proteome-wide analysis using SILAC-iodoTMT workflow to quantitatively estimate level sulfhydryl...
Under normal conditions, the cellular redox status is maintained in a steady state by reduction and oxidation processes. These alterations cell are mainly sensed protein thiol residues of cysteines thus regulating function. The imbalance homeostasis may therefore regulate turnover either directly modulating transcription factors or indirectly degradation damaged proteins due to oxidation. A new analytical method capable simultaneously assessing expression cysteine would provide valuable tool...
Fetal and adult hematopoietic stem progenitor cells (HSPCs) are characterized by distinct redox homeostasis that may influence their differential cellular behavior in normal malignant hematopoiesis. In this work, we have applied a quantitative mass spectrometry-based proteomic approach to comprehensively describe reversible cysteine modifications primary mouse fetal HSPCs. We defined the state of 4,438 cysteines HSPCs demonstrated higher susceptibility oxidation protein thiols Our data...
Tacrine was withdrawn from clinical use as a drug against Alzheimer's disease in 2013, mainly due to drug-induced liver injury. The culprit of tacrine-associated hepatotoxicity is believed be the 7-OH-tacrine metabolite, possible precursor quinone methide (Qmeth), which binds intracellular -SH proteins. In our study, several different animal and human models (liver microsomes, primary hepatocytes, slices) were used investigate biotransformation tacrine its 7-substituted analogues...
Our recent exploratory proteomic study suggested increased levels of neutrophil-gelatinase associated lipocalin (P80188, NGAL_HUMAN) due to microbial invasion the amniotic cavity (MIAC) and histological chorioamnionitis (HCA) in women with preterm prelabor rupture membranes. In this study, we verified proteomics findings by assessing fluid NGAL ELISA original cohort. The level was significantly higher positive for both MIAC HCA compared conditions ruled out (median 75.1 ng/ml versus 27.9...
Spontaneous preterm birth significantly contributes to the overall neonatal morbidity associated with deliveries. Nearly 50% of cases are microbial invasion amniotic cavity followed by an inflammatory response. Robust diagnostic tools for neonates jeopardized infection and inflammation may thus decrease substantially. Amniotic fluid retrieved during labor retains fetal pregnancy-related protein fingerprint its sampling does not place any unwanted stress on women. Using exploratory targeted...
AimTo develop and test a protocol for isolation of potential auto-antigens from chorionic tissue that may be linked to recurrent miscarriage (RM).MethodsThe strategy included: 1) IgGs tightly bound RM patients by protein G chromatography; 2) construction affinity columns using the antibodies auto-antigens; 3) enrichment detergent extracted solution proteins 4) separation dodecyl sulfate-electrophoresis followed matrix-assisted laser desorption/ionization-time flight mass spectrometry...
Abstract Preterm prelabour rupture of membranes beyond the 34th week gestation (late PPROM) is frequently associated with risk microbial invasion amniotic fluid (MIAC) and histological chorioamnionitis (HCA). Hence, we employed a Tandem Mass Tag-based approach to uncover proteome response presence MIAC HCA in late PPROM. Protein dysregulation was only five cases group 15 women confirmed HCA. Altogether, 138 proteins were changed these exclusively. These particularly excessive neutrophil...
Post-translational modifications, including phosphorylation, greatly impact the physiological function of proteins, especially those that are natively unfolded and implicated in many neurodegenerative diseases. However, structural functional studies such proteins require fully defined not physiological. Thus, kinases ERK2 GSK-3β were immobilized to various superparamagnetic beads with carboxylic, aldehyde, Ni2+, or Co3+ groups, a view efficiently phosphorylate peptides vitro. Full...
To determine amniotic fluid myeloperoxidase concentration in women with preterm prelabor rupture of the membranes microbial invasion cavity and histological chorioamnionitis.One hundred eighty-one singleton pregnancies a gestational age between 24+0 36+6 weeks were included this study. Amniocenteses performed, was determined using ELISA.Women had higher median than without condition (149.2 ng/mL vs. 54.6 ng/mL; p = 0.0006). Women presence chorioamnionitis (103.7 50.0 0.0001). The both...
Objective The aim of this study was to identify early proteomic biomarkers spontaneous preterm delivery (PTD) in mid-trimester amniotic fluid from asymptomatic women. Methods This is a case-cohort study. Amniotic genetic amniocentesis (14–19 weeks gestation) collected 2008 2011. analysis conducted 24 healthy women with subsequent PTD (cases) and 40 randomly selected delivering at term (controls). An exploratory phase proteomics pooled samples followed by verification ELISA individual case...
Background Amniotic fluid is clinically accessible via amniocentesis and its protein composition may correspond to birth timing. Early changes in the amniotic proteome could therefore be associated with subsequent development of spontaneous preterm delivery. Objective The main objective this study was perform unbiased proteomics analysis association between mid-trimester delivery gestational duration, respectively. A secondary validate replicate findings by enzyme-linked immunosorbent assay...
Anesthesia is regarded as an important milestone in medicine. However, the negative effect on memory and learning has been observed. In addition, impact of anesthetics postoperative cognitive functions still discussed. this work, vivo experiment simulating a general anesthesia ICU sedation was designed to assess two intravenous (midazolam, dexmedetomidine) inhalational (isoflurane, desflurane) agents neuronal centers for cognition (neocortex), learning, (hippocampus). More than 3600 proteins...