A5016049158- Mycobacterium research and diagnosis
- Clostridium difficile and Clostridium perfringens research
- Orthopedic Infections and Treatments
- Central Venous Catheters and Hemodialysis
- Oral and Craniofacial Lesions
- Infant Nutrition and Health
- Nosocomial Infections in ICU
- Antibiotics Pharmacokinetics and Efficacy
- Bone and Dental Protein Studies
- Ocular Infections and Treatments
- Tuberculosis Research and Epidemiology
- Infectious Diseases and Tuberculosis
- Infectious Diseases and Mycology
- Nephrotoxicity and Medicinal Plants
- Antibiotic Resistance in Bacteria
- Ocular Diseases and Behçet’s Syndrome
- Antibiotic Use and Resistance
- Microbial Metabolism and Applications
- Bacterial Infections and Vaccines
- Photodynamic Therapy Research Studies
- Urinary Tract Infections Management
- Microscopic Colitis
- Bartonella species infections research
- Oral Health Pathology and Treatment
- Antimicrobial Resistance in Staphylococcus
Charles University
2014-2025
University Hospital Hradec Králové
2010-2025
Research Institute of Inorganic Chemistry
2022
Opća bolnica Karlovac
2020
Karlovac University of Applied Sciences
2020
Ocní klinika
2013
Abstract Background Antimicrobial lock therapy is recommended for preventing and treating catheter‐related bloodstream infections, but different solutions have uncertain efficacy. Methods Two locks, 1.35% taurolidine 4% ethylenediaminetetraacetic acid (EDTA), were tested on Staphylococcus epidermidis , aureus methicillin‐resistant S. Pseudomonas aeruginosa multidrug‐resistant P. vancomycin‐resistant Enterococcus faecium Klebsiella oxytoca (carbapenemase producing), K. pneumoniae...
Abstract Photodynamic antimicrobial therapy (PACT) involves the utilisation of photosensitizers activated by exposure to visible light in order eradicate microbes (this method has already been applied photodynamic tumours). effect particular photosensitive substance (PS) is attributed its ability penetrate susceptible microorganisms, absorb certain wavelength, and generate reactive cytotoxic oxygen products. The target microorganisms for photoinactivation are bacteria, fungi, viruses...
Against a background of rapid increase β-lactamase-producing or multi-resistant pathogenic bacteria and the resulting lack effective antibiotic treatment, some older antibiotics have been tested for new therapeutic uses. One these is fosfomycin, to which according studies resistant are very sensitive. Our study was designed because there no data on fosfomycin susceptibility rate in Czech Republic. In this from January 2013 June 2014 3295 unique isolates Gram-negative had caused urinary tract...
Abstract
Abstract Objectives To investigate the relationship between Clostridium (Clostridioides) difficile strain characteristics and C. infection (CDI) outcome. Methods Between October December 2017, 16 hospitals collected epidemiological data according to European Centre for Disease Prevention Control (ECDC) surveillance protocol CDI. isolates were characterized by ribotyping, toxin genes detection antibiotic susceptibility testing metronidazole, vancomycin moxifloxacin. Results The overall mean...
ABSTRACT A 2-year national genomic screening in the Czech Republic identified a notable prevalence of New Delhi metallo-β-lactamase 5 (NDM-5)-producing Escherichia coli sequence type 38 (ST38) city Brno. Forty-two ST38 E. isolates harbored bla NDM-5 gene on chromosome. Virulence factors confirmed persistence these through biofilm formation. Single Nucleotide Polymorphisms (SNPs)-based phylogeny and CRISPR assay typing showed minimal variations, implying clonally driven outbreak. Results...
The molecular mechanisms linking obstructive sleep apnea syndrome (OSA) to obesity and the development of metabolic diseases are still poorly understood. role hypoxia (a characteristic feature OSA) in excessive fat accumulation has been proposed. present study investigated possible effects (4% oxygen) on de novo lipogenesis by tracking major carbon sources differentiating 3T3-L1 adipocytes. Gas-permeable cultuware was employed cultivate adipocytes (4%) for 7 or 14 days differentiation. We...
Musculoskeletal infections remain a major complication in orthopedic surgery. The local delivery of antibiotics provides the high levels required to treat an infection without systemic toxicity. However, toxicity antibiotic carriers mesenchymal stem cells, as result both peak concentrations and type carrier, may be significant.To address this concern, elution kinetics vancomycin gentamicin from several commercially available impregnated by surgeon (10 ml each sterile carrier were manually...
Abstract Background Health problems of premature infants can affect both general and oral health. The enamel defects, poor dietary hygiene habits may predispose these children to dental caries. This study was conducted assess the impact very low birth weight prematurity on caries risk in early childhood. Methods cohort consists 189 one year old infants. Anamnestic data were obtained from hospital records, feeding practice, bed-time sugar drinks onset questionnaires. Saliva samples their...
Abstract We report the case of isolation Bordetella trematum from respiratory tract a patient with lung carcinoma. This gram-negative, opportunistic rod was firstly described in 1996. To date, only several strains have been isolated and reported, mostly skin soft tissue infections. The admitted to ICU Pulmonary Department incipient septic shock failure. Intravenous fluid resuscitation non-invasive ventilation were administered immediately. A broad spectrum antibiotic piperacillin/tazobactam...
Infections caused by Mycobacterium setense or Clostridium celerecrescens are extremely rare. In this report, for the first time a dual infection with these two pathogens is described. An 18-year-old female suffered multiple injuries, including an open comminuted fracture of right humeral diaphysis after falling from fifth-floor balcony in January 2019. Five months accident, fistula appeared scar, reaching bone tissue. M. and C. were cultured sinus swabs subsequently perioperative samples....
PURPOSE OF THE STUDYA very serious complication following joint replacement surgery is periprosthetic infection that can be caused by a urinary tract infection.Insertion of an indwelling catheter constitutes risk factor may result in infections.The aim this prospective randomised study was to compare the occurrence significant bacteriuria and symptomatic infections during antibiotic prophylaxis at time removal cotrimoxazole two doses with no administration antibiotics.We also monitored...
Although Francisella (F.) tularensis is a well-described and understood zoonotic pathogen, its importance in Central Europe relatively minor and, as such, tularaemia may be missed the differential diagnosis. The annual incidence of Czech Republic stable with up to 100 reported cases per year, except epidemic years 1998 1999 225 222 cases, respectively. It is, however, higher comparison neighbouring countries. common route transmission handling infected animals. Tularaemia not commonly...
Mycobacterium chelonae, a rapidly growing nontuberculous mycobacterium, is usually described as causative agent of soft tissue infections (postsurgical, posttraumatic, posttransplantation, postinjection, catheter infection, etc.), but only rarely cause osteomyelitis. The authors describe case report 72-year-old man with osteomyelitis the talus. Initially, infection was assessed without any osteolytic changes on X-ray. After cultivation subsequent targeted molecular typing rpoB gene, M....
Mycobacterioses are less frequently occurring but serious diseases. In recent years, at a global level, the incidence of mycobacterioses induced by rapidly growing species Mycobacterium abscessus (M. a.), which is considered to be most resistant antibiotics and difficult treat, has been on rise. Correct identification level subspecies a. abscessus, M. massiliense, bolletii) determination its sensitivity macrolides, basis combination therapy, principal importance for management disease. We...
Introduction: Nontuberculous mycobacterial (NTM) infection of lymph nodes in young children is a rare disease. The increasing incidence has been observed after termination vacciation Czech Republic November 2010. Aim the study: To provide an overview clinical picture, dia gnostic options and therapeutic approach with neck aff ected by non-tuberculous mycobacteria. Methods: Retrospective cohort study patients confi rmed NTM treated at Clinic Otorhinolaryngology Head Neck Surgery Faculty...