A5080816340- Aquaculture disease management and microbiota
- Antifungal resistance and susceptibility
- Colorectal and Anal Carcinomas
- Colorectal Cancer Treatments and Studies
- Gastric Cancer Management and Outcomes
- Colorectal Cancer Surgical Treatments
- Microbial infections and disease research
- Antibiotic Resistance in Bacteria
- Biopolymer Synthesis and Applications
- Vibrio bacteria research studies
- Cytokine Signaling Pathways and Interactions
- Neuroblastoma Research and Treatments
- Cervical Cancer and HPV Research
- Animal Virus Infections Studies
- Urinary and Genital Oncology Studies
- Genomics and Phylogenetic Studies
- Melanoma and MAPK Pathways
- Neuroendocrine Tumor Research Advances
- Parasitic infections in humans and animals
- Fungal Infections and Studies
- Analytical Chemistry and Chromatography
- Breast Cancer Treatment Studies
- Ovarian cancer diagnosis and treatment
- Parasitic Infections and Diagnostics
- Genetic factors in colorectal cancer
Public Health Institute Ostrava
2006-2024
Institute of Public Health Zadar
2024
University of Ostrava
2018-2023
Národný Onkologický Ústav
2021
Masaryk University
2015-2021
Public Health Institute
2021
Masaryk Memorial Cancer Institute
2003-2015
Palacký University Olomouc
2011
Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures
2006
The multiple forms of pulmonary aspergillosis caused by Aspergillus species are the most common respiratory mycoses. Although invasive, analysis diagnostic biomarkers in bronchoalveolar lavage fluid (BALF) is a clinical standard for diagnosing these conditions. BALF samples from 22 patients with proven or probable were assayed human pentraxin 3 (Ptx3), fungal ferricrocin (Fc), and triacetylfusarinine C (TafC) retrospective study. infected group included invasive (IPA) chronic (CPA). data...
In acutely ill patients, particularly in intensive care units or mixed infections, time to a microbe-specific diagnosis is critical successful outcome of therapy. We report the application evolving technologies involving mass spectrometry diagnose and monitor patient’s course. As proof this concept, we studied five patients used two rat models mono-infection coinfection. noninvasive combined monitoring Aspergillus fumigatus Pseudomonas aeruginosa infection. The invasive coinfection was...
ABSTRACT A 2-year national genomic screening in the Czech Republic identified a notable prevalence of New Delhi metallo-β-lactamase 5 (NDM-5)-producing Escherichia coli sequence type 38 (ST38) city Brno. Forty-two ST38 E. isolates harbored bla NDM-5 gene on chromosome. Virulence factors confirmed persistence these through biofilm formation. Single Nucleotide Polymorphisms (SNPs)-based phylogeny and CRISPR assay typing showed minimal variations, implying clonally driven outbreak. Results...
Invasive pulmonary aspergillosis (IPA) may be a rare cause of granulomatous pneumonia in horses. The mortality IPA is almost 100%; direct diagnostic tools horses are needed. Bronchoalveolar lavage fluid (BALF) and serum samples were collected from 18 horses, including individuals suffering (n = 1), equine asthma (EA, n 12), 5 healthy controls. Serum another 6 Samples BALF 18) analyzed for Aspergillus spp. DNA, fungal galactomannan (GM), ferricrocin (Fc), triacetylfusarinin C (TafC),...
In 2001, a Gram-variable, facultatively anaerobic, endospore-forming bacterium isolated from biodeteriorated mural paintings in the Servilia tomb of Roman necropolis Carmona was deposited as Paenibacillus strain LMG 19508. Subsequently, characterized detail using phenotypic and molecular methods. The 16S rRNA gene sequence confirmed that belongs to genus indicated its relationship mendelii CCM 4839(T) (96.7 % similarity). predominant menaquinone MK-7. cell wall contained meso-diaminopimelic...
ABSTRACT Using the established commercial system Sherlock (MIDI, Inc.), cellular fatty acid methyl ester analysis for differentiation among Burkholderia cepacia complex species was proven. The identification key based on diagnostic acids is able to discern phenotypically related Ralstonia pickettii and Pandoraea spp. further distinguish pyrrocinia , ambifaria vietnamiensis .
The proliferative potential of six primary ovarian carcinoids with different clinical outcome and histogenetic origin was examined immunohistochemically. results showed that two cases extremely high level activity were associated metastatic spread. In the remaining tumors, factor found to be at low comparable excellent prognosis typical in other locations. preliminary a possibility prediction according typing into categories, i.e., tumors intermediate malignancy. Topoisomerase II-alpha Ki-67...
In some patients, granulomatous reactions around foreign-bodies with FDG-PET positivity may be misinterpreted as malignancy. We report a case of 31-year-old man diagnosed testicular germinal tumor retroperitoneal and left supraclavicular metastases. The diagnosis was based on lymph node biopsy. Restaging examinations after orchiectomy chemotherapy showed normalization markers significant regression the infiltrate, however, residual positive lesion in fossa. etiology behind this infiltration...
New biomarker panel was developed and validated on 165 critically ill adult patients to enable a more accurate invasive candidiasis (IC) diagnosis. Serum levels of the panfungal (1,3)-β-D-glucan (BDG) inflammatory biomarkers C-reactive protein, presepsin (PSEP), procalcitonin (PCT) were correlated with culture-confirmed candidemia or bacteremia in 58 107 patients, respectively. The diagnostic utility evaluated sensitivity, specificity, positive predictive value (PPV), negative (NPV). BDG...
The resistance to interferons (IFNs) limits their anticancer therapeutic efficacy. Here we studied the antiproliferative effect of interferon gamma in relation SOCS3 expression a panel breast cancer cell lines and normal mammary epithelial cells. Compared cells most (7/8) were highly resistant IFN-gamma. Using Northern blot real time RT-PCR investigated transcription genes. All (4/4) showed induction (2-14 fold) while did not or weakly activated after treatment. Among lines, MDA-MB-468...
A retrospective analysis of consecutive patients (183 in total, which 105 were males and 78 females) with gastrointestinal stromal tumour (GIST) was performed.The mean age 61 years, median 64 years.The most frequent localization the stomach 74 (40.4 %) small intestine 46 (25.1 %).Two or more different synchronous metachronous cancers occurred 34 (18.6 histologically confi rmed GIST.Ninety-six treated imatinib mesylate palliative setting during course their disease.The therapy fi nished 60 36...
In breast cancer (BC), several cytogenetic markers are routinely investigated (Her2, progesterone and estrogen receptors) that distribute patients into different diagnostic groups, determine prognosis predict effectiveness of therapy. Additional genetic can improve prediction or in BC patients. C-MYC CCND1 gene aberrations frequently found were chosen for this study.