A5085994708- Viral Infectious Diseases and Gene Expression in Insects
- Insect Resistance and Genetics
- Plant Virus Research Studies
- Virus-based gene therapy research
- Animal Virus Infections Studies
- Transgenic Plants and Applications
- Entomopathogenic Microorganisms in Pest Control
- SARS-CoV-2 and COVID-19 Research
- Monoclonal and Polyclonal Antibodies Research
- Protein purification and stability
- Viral gastroenteritis research and epidemiology
- Insect and Pesticide Research
- Influenza Virus Research Studies
- Graphene and Nanomaterials Applications
- Invertebrate Immune Response Mechanisms
- Plant Disease Resistance and Genetics
- Respiratory viral infections research
- 3D Printing in Biomedical Research
- Analytical Chemistry and Sensors
- Vector-Borne Animal Diseases
- Electrochemical sensors and biosensors
- Plant and Fungal Interactions Research
- Autophagy in Disease and Therapy
- Advanced Biosensing Techniques and Applications
- Long-Term Effects of COVID-19
University of Oxford
2024
Institute of Molecular Biology, Academia Sinica
2012-2023
National Chung Hsing University
2009-2023
National Taiwan University
1994-2023
National Defense Medical Center
2008-2021
Academia Sinica
2004-2021
National Kaohsiung University of Applied Sciences
2015-2017
Soochow University
2016
Tung Fang Design Institute
2015
National Quemoy University
2015
Single-walled carbon nanotubes (SWNTs) with various unique properties have attracted great attention in cancer theranostics. Herein, SWNTs are coated a shell of polydopamine (PDA), which is further modified by polyethylene glycol (PEG). The PDA the obtained SWNT@PDA-PEG could chelate Mn(2+), together metallic nanoparticulate impurities anchored on offer enhanced both T1 and T2 contrasts under magnetic resonance (MR) imaging. Meanwhile, also utilizing shell, radionuclide (131)I be easily...
Abstract In plants, sugars are required to sustain growth and regulate gene expression. A large set of genes either up- or down-regulated by sugars; however, whether there is a common mechanism signal transduction pathway for differential coordinated sugar regulation remain unclear. the present study, rice (Oryza sativa cv Tainan 5) cell culture was used as model system address this question. Sucrose glucose both played dual functions in exemplified up-regulation growth-related...
Titer determination is a prerequisite for the study of viruses. However, current available methods are tedious and time-consuming. To improve efficiency titer determination, we have developed rapid simple method routine detection baculovirus titers using quantitative real-time PCR. This based on amplification approximately 150-bp fragments located in coding regions selected genes. The PCR was found to be range 103 109 virus particles per 200 μL supernatant, results were closely correlated...
Abstract The inflammatory response and the intracellular signaling pathway induced by severe acute respiratory syndrome (SARS)-coronavirus (CoV) were studied in lung epithelial cells fibroblasts. SARS-CoV spike (S) protein-encoding plasmid activations of IL-8 promoter AP-1, but not NF-κB these cells. Mutation κB site, abolished S protein-induced activity. release was effectively vAtEpGS688, a baculovirus exhibiting aa 17–688 fragment protein, this induction attenuated angiotensin-converting...
Rationale: Cisplatin (CDDP) is a broad-spectrum anticancer drug but chemoresistance to CDDP impedes its wide use for cancer therapy. Autophagy an event occurring in the cytoplasm and cytoplasmic LC3 puncta formation hallmark of autophagy. Graphene oxide (GO) nanomaterial that provokes autophagy CT26 colon cells confers antitumor effects. Here we aimed evaluate whether combined GO with (GO/CDDP) overcomes different uncover underlying mechanism. Methods: We treated GO/CDDP evaluated...
Monkeypox (mpox), a zoonotic disease, has rapidly spread globally, prompting the WHO to declare it public health emergency. The long incubation period, early symptoms resembling respiratory infections, and diagnostic challenges hinder timely epidemic control accurate clinical diagnosis. monkeypox virus (MPXV) encodes A29L protein, which binds cellular heparan sulfate facilitate infection serves as target for treatment diagnostics. Thus, developing effective tools treatments is critically...
E C Stephenson, Y Chao, and J D Fackenthal Department of Biology, University Rochester, New York 14627.
Cellulases from Bacillus and Geobacillus bacteria are potentially useful in the biofuel animal feed industries. One of unique characteristics these enzymes is that they usually quite thermostable. We previously identified a cellulase, GsCelA, thermophilic sp. 70PC53, which much more thermostable than its homolog, BsCel5A. Thus, two cellulases provide pair structures ideal for investigating mechanism regarding how can retain activity at high temperature. In present study, we applied SCHEMA...
A new variant of the porcine epidemic diarrhea virus (PEDV) is an emerging swine disease, killing considerable numbers neonatal piglets in North America and Asia recent years. To generate immunogens mimicking complex spike (S) protein folding with proper posttranslational modification to mount a robust immune response against highly virulent PEDV, two baculoviruses displaying full-length S (S-Bac) S1 (S1-Bac) Taiwan genotype 2b (G2b) PEDV Pintung 52 (PEDV-PT) strain were constructed....
Abstract Purpose The aim of this study was to elucidate the factors associated with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that may initiate cytokine cascades and correlate clinical characteristics patients disease 2019 (COVID-19) their serum profiles. Methods Recombinant baculoviruses displaying SARS-CoV-2 spike or nucleocapsid protein were constructed transfected into A549 cells THP-1-derived macrophages, determine which release. SARS-CoV-2-specific antibody titers...
Heliothis zea nudivirus-1 (HzNV-1) is an insect virus previously known as Hz-1 baculovirus. One of its major early genes, hhi1, responsible for the establishment productive viral infection; another gene, pag1, which expresses a non-coding RNA, only transcript detectable during latency. Here we showed that this RNA was further processed into at least two distinct miRNAs, targeted and degraded hhi1 transcript. This result strikingly similar to recent report herpes simplex produces...
ABSTRACT Persistent/latent viral infections of insect cells are a prominent though poorly understood phenomenon. In this study, the long-term association between Hz-1 virus and host cells, conventionally referred to as persistent infection, is described. With aid newly developed fluorescent cell-labeling system, we found that productive replication occurs by spontaneous reactivation in fewer than 0.2% persistently infected cell lines over 5-day period. Once takes place, dies. The contain...
The 5' regulatory region and putative signal sequence of a rice alpha-amylase gene, alpha Amy8, was fused to bacterial gene encoding beta-glucuronidase (GUS) introduced into rice, tobacco, potato via Agrobacterium-mediated transformation systems. Expression this chimeric in suspension cells transgenic plants suppressed by the presence sucrose medium induced its absence. Induction or suppression GUS expression could be reversed deprivation replenishment, respectively, medium. expressed fusion...
The severe acute respiratory syndrome-associated coronavirus (SARS-CoV) uses dendritic cell-specific ICAM-3 grabbing nonintegrin (DC-SIGN) to facilitate cell entry via cellular receptor-angiotensin-converting enzyme 2. For this project, we used recombinant baculoviruses expressing different lengths of SARS-CoV spike (S) protein in a capture assay deduce the minimal DC-SIGN binding region. Our results identified region location between amino acid (aa) residues 324 386 S protein. We then...
Efficient gene delivery technologies play an essential role in the functional analyses that are necessary for basic and applied researches. Mosquitoes ubiquitous insects, responsible transmitting many deadly arboviruses causing millions of human deaths every year. The lack efficient flexible strategies mosquitoes among major hurdles study mosquito biology mosquito-pathogen interactions. We found Autographa californica multiple nucleopolyhedrovirus (AcMNPV), type baculovirus species, can...
COVID-19 in humans is caused by Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) that belongs to the beta family of coronaviruses. SARS-CoV-2 causes severe illness 10–15% infected individuals and mortality 2–3%. Vaccines are urgently needed prevent infection contain viral spread. Although several mRNA- adenovirus-based vaccines highly effective, their dependence on “cold chain” transportation makes global vaccination a difficult task. In this context, stable lyophilized vaccine...
A DNA sequence upstream from the polyhedrin gene of baculovirus Autographa californicanucleopolyhedrovirus (AcMNPV) was found to activate strongly expression full or minimal promoters derived AcMNPV and other sources. Promoters tested included CMV (CMVm) promoter human cytomegalovirus, heat shock 70 fromDrosophila, p35 baculovirus. Deletion mutagenesis analyses showed that this functional (pu) activator contains three open reading frames (ORFs), ORF4, ORF5, andlef2. In plasmid transfection...
Autographa californica multiple nucleopolyhedrovirus (AcMNPV), the type species of family Baculoviridae, is an insect-specific virus that can enter a variety mammalian cells. The potential this versatile for protein expression or gene therapy in cells has become focus many studies. In most cells, transduced AcMNPV genes are either not expressed at extremely low level. Here, we studied effects two major trans-activators, IE1 and IE2, on activation genome Vero E6 Microarray analysis showed...