A5054407917- Cardiac electrophysiology and arrhythmias
- Cardiac Arrhythmias and Treatments
- Cardiac Ischemia and Reperfusion
- Electrostatic Discharge in Electronics
- Ion channel regulation and function
- Cardiac Arrest and Resuscitation
- Microbial Inactivation Methods
- Neuroscience and Neural Engineering
- Receptor Mechanisms and Signaling
- Cardiac pacing and defibrillation studies
- Cardiomyopathy and Myosin Studies
- Neuropeptides and Animal Physiology
- Anesthesia and Neurotoxicity Research
- Thermal Regulation in Medicine
- Atrial Fibrillation Management and Outcomes
- Laser-induced spectroscopy and plasma
- Viral Infections and Immunology Research
- Musculoskeletal pain and rehabilitation
- Respiratory Support and Mechanisms
- Anesthesia and Pain Management
- Cardiovascular Function and Risk Factors
- Pain Mechanisms and Treatments
- Microfluidic and Bio-sensing Technologies
- Connexins and lens biology
- Pluripotent Stem Cells Research
Medtronic (Ireland)
2006-2024
Medtronic (United States)
2005-2024
FocusStart (United States)
2015-2018
University of Minnesota
2000-2016
Integrative Medicine Institute
2011
Queensland University of Technology
2009
The University of Queensland
2009
University of Nottingham
2009
University of Milan
2009
Medtronic (Italy)
2009
Although we know much about the molecular makeup of sinus node (SN) in small mammals, little is known it humans. The aims present study were to investigate expression ion channels human SN and use data predict electrical activity.Quantitative polymerase chain reaction, situ hybridization, immunofluorescence used analyze 6 tissue samples. Messenger RNA (mRNA) for 120 (and some related proteins) was measured SN, a novel paranodal area, right atrium (RA). results showed, example, that compared...
Contact force has been used to titrate lesion formation for radiofrequency ablation. Pulsed field ablation (PFA) is a field-based technology which limited evidence on the impact of contact size available.Porcine hearts (n = 6) were perfused using modified Langendorff set-up. A prototype focal PFA catheter attached gauge was held perpendicular epicardium and lowered until made. recorded during each delivery. Matured lesions cross-sectioned, stained, dimensions measured.A total 82 evaluated...
Pulsed field ablation (PFA) is a novel energy modality for treatment of cardiac arrhythmias. The impact electrode-tissue proximity on lesion formation by PFA has not been conclusively assessed. objective this investigation was to evaluate the effects with biphasic, bipolar system.PFA delivered ventricular epicardial surface in an isolated porcine heart model (n=8) via 4-electrode prototype catheter. An offset tool designed control distance between electrodes and target tissue; deliveries...
Phrenic nerve palsy is a well-known complication of cardiac ablation, resulting from the application direct thermal energy. Emerging pulsed field ablation (PFA) may reduce risk phrenic injury but has not been well characterized.Accelerometers and continuous pacing were used during PFA deliveries in porcine model. Acute dose response was established first experimental phase with ascending intensity delivered to (n=12). In second phase, nerves targeted single level observe effect repetitive...
Abstract We have previously shown in rabbit that the pacemaker of heart (the sinus node) is widespread and matches wide distribution leading site within right atrium. There is, however, uncertainty about precise location human heart, its spatial relationships with surrounding atrial muscle. Therefore, aim current study was to investigate node tissue a series healthy hearts and, for one construct computer three‐dimensional anatomical model node, including likely orientation myocytes. A...
Cardiac gene therapy has emerged as a promising option to treat advanced heart failure (HF). Advances in molecular biology and targeting approaches are offering further novel options for genetic manipulation of the cardiovascular system. The aim this study was improve cardiac function chronic HF by overexpressing constitutively active inhibitor-1 (I-1c) using cardiotropic vector generated capsid reengineering adeno-associated virus (BNP116). One month after large anterior myocardial...
Pulsed field ablation is an emerging modality for catheter-based cardiac ablation. The main mechanism of action irreversible electroporation (IRE), a threshold-based phenomenon in which cells die after exposure to intense pulsed electric fields. Lethal threshold IRE tissue property that determines treatment feasibility and enables the development new devices therapeutic applications, but it greatly dependent on number pulses their duration.In study, lesions were generated by applying porcine...
Basic and clinical evidence suggests that omega-3 (n-3) polyunsaturated fatty acids (PUFAs) decrease fatal arrhythmias infarct sizes. This study investigated if pericardial delivery of n-3 PUFAs would protect the myocardium from ischemic damages arrhythmias. Acute myocardial infarctions were induced in 23 pigs with either 45 min balloon inflations or clamp occlusions left anterior descending coronary arteries 180 reperfusion. Docosahexaenoic acid (C22:6n-3, DHA, mg), one main fish oil, was...
Irreversible electroporation is an energy form utilizing high-voltage pulsed electric field, leading to cellular homeostasis disruption and cell death. Recently, irreversible has shown promising results for the treatment of cardiac arrhythmias. However, reversible effects field on myocytes remain poorly understood. Here, we evaluated influence a monophasic single pulse (EP) contractility, Ca2+ recovery myocytes.Isolated rat left ventricular were electroporated using EP different durations...
Opioids are involved in cardiac ischemic preconditioning. Important species differences cellular signaling mechanisms, antiarrhythmic, and antistunning effects have been described. The role of the delta-opioid receptor activation swine remains unknown. Forty minutes before a 45-min occlusion 180-min reperfusion left anterior descending coronary artery, open-chest, pentobarbital-anesthetized received either 1) saline (controls); 2) [D-Ala(2),D-Leu(5)]enkephalin (DADLE); 3)...
Glucagon-like peptide-1 (GLP-1) is a hormone predominately synthesized and secreted by intestinal L-cells. GLP-1 modulates multiple cellular functions its receptor agonists are now used clinically for diabetic treatment. Interestingly, preclinical clinical evidence suggests that produce beneficial effects on dysfunctional hearts via acting myocardial receptors. As the of myocyte electrophysiology largely unknown, this study was to assess if could affect cardiac voltage-gated L-type Ca2+...
Electroporation is a biophysical phenomenon involving an increase in cell membrane permeability to molecules after high-pulsed electric field applied the tissue. Currently, electroporation being developed for non-thermal ablation of cardiac tissue treat arrhythmias. Cardiomyocytes have been shown be more affected by when oriented with their long axis parallel field. However, recent studies demonstrate that preferentially orientation depends on pulse parameters. To gain better insight into...
The goal of our study was to determine the importance electric field orientation in an anisotropic muscle tissue for extent irreversible electroporation damage by means experimentally validated mathematical model.Electrical pulses were delivered porcine skeletal vivo inserting needle electrodes so that applied direction either parallel or perpendicular fibres. Triphenyl tetrazolium chloride staining used shape lesions. Next, we a single cell model cell-level conductivity during...
Electroporation is used in medicine for drug and gene delivery, as a nonthermal ablation method tumor treatment cardiac ablation. involves delivering high-voltage electric pulses to target tissue; however, this can cause effects beyond the intended tissue like nerve stimulation, muscle contractions pain, requiring use of sedatives or anesthetics. It was previously shown that adjusting pulse parameters may mitigate some these effects, but not how adjustments would affect electroporation’s...
Delivery of electrical energy for sensing or therapeutic purposes often involves electrochemical phenomena at the electrode-electrolyte solution interface. Release gaseous bubbles that accompanies delivery pulsed electric fields to tissues in applications such as electrochemotherapy tumours and irreversible electroporation field ablation cardiac electrophysiology needs be understood characterized. We present an vitro study using into saline, employing multiple different treatment protocols,...
Pharmacological preconditioning with κ-opioid receptor agonists is proarrhythmic and exerts antipreconditioning effects in rats. In swine, it unknown whether stimulation plays a role pharmacological preconditioning. Swine were preconditioned 1) saline (controls), 2) [d-Ala 2 ,d-Leu 5 ]enkephalin (DADLE), 3) morphine, 4) pentazocine, 5) norbinaltorphimine (nor-BNI), 6) DADLE + nor-BNI, 7) morphine or 8) pentazocine nor-BNI before occlusion (45 min) reperfusion (180 of the left anterior...
When sinus node or atrioventricular (AV) cells are damaged by disease, the implantation of an artificial cardiac pacemaker becomes necessary. In search for a biological alternative, objective this study was to demonstrate whether in vivo adenoviral gene transfer Adenylate-Cyclase type VI (AC-VI) can create activity porcine AV block model. Genetic therapy arrhythmic disorders heart has been subject extensive studies. Cyclic AMP is generated response Beta-adrenergic receptor stimulation and...
A decrease of 1–2°C core temperature provides protection against cerebral ischemia. However, shivering usually prevents reduction in unanesthetized patients. Therefore, it was tested whether facial and airway heating increases the threshold enables cooling Nine trials were performed on seven healthy male volunteers. Each subject positioned supine a circulating-water mattress (8–15°C) with convective-air coverlet (15–18°C) extending from neck to feet. dynamic study protocol governed by...
Ischemic preconditioning has been utilized to protect the heart from ischemia prior onset, whereas postconditioning is employed minimize consequences of at onset reperfusion. The underlying mechanisms and pathways ischemic pre- continue be investigated as therapeutic targets. We evaluated administration a delta opioid agonist or cariporide on various parameters associated with myocardial reperfusion injury upon isolated porcine hearts. hearts were reperfused in vitro Krebs buffer containing...