A5043271142- Cardiac electrophysiology and arrhythmias
- Ion channel regulation and function
- ECG Monitoring and Analysis
- Cardiac Arrhythmias and Treatments
- Neuroscience and Neural Engineering
- Atrial Fibrillation Management and Outcomes
- Cardiovascular Function and Risk Factors
- Cardiomyopathy and Myosin Studies
- Receptor Mechanisms and Signaling
- Cardiac pacing and defibrillation studies
- EEG and Brain-Computer Interfaces
- Advanced MRI Techniques and Applications
- Heart Rate Variability and Autonomic Control
- Cardiac Imaging and Diagnostics
- Medical Image Segmentation Techniques
- Phonocardiography and Auscultation Techniques
- Cardiac Valve Diseases and Treatments
- Advanced Neural Network Applications
- Cardiovascular Effects of Exercise
- Electrochemical Analysis and Applications
- Non-Invasive Vital Sign Monitoring
- Advanced X-ray and CT Imaging
- stochastic dynamics and bifurcation
- Neurological disorders and treatments
- Circadian rhythm and melatonin
University of Manchester
2015-2024
Southwest Medical University
2017-2024
Beijing Academy of Artificial Intelligence
2023-2024
Guangdong Academy of Sciences
2024
Peng Cheng Laboratory
2019-2023
Southern Medical University
2023
Ocean University of China
2022
Qingdao National Laboratory for Marine Science and Technology
2019-2022
UK Astronomy Technology Centre
2022
Harbin Institute of Technology
2012-2021
Manganese superoxide dismutase (SOD2) converts to oxygen plus hydrogen peroxide and serves as the primary defense against mitochondrial superoxide. Impaired SOD2 activity in humans has been associated with several chronic diseases, including ovarian cancer type I diabetes, overexpression appears suppress malignancy cultured cells. We have produced a line of knockout mice (SOD2m1BCM/SOD2m1BCM) that survive up 3 weeks age exhibit novel pathologic phenotypes severe anemia, degeneration neurons...
Heart rate (HR) variability (HRV; beat-to-beat changes in the R-wave to interval) has attracted considerable attention during past 30+ years (PubMed currently lists >17 000 publications). Clinically, a decrease HRV is correlated higher morbidity and mortality diverse conditions, from heart disease fetal distress. It usually attributed fluctuation cardiac autonomic nerve activity. We calculated parameters variety of preparations (including humans, living animals, Langendorff-perfused heart,...
Cardiac arrhythmia is associated with abnormal electrical activities of the heart, which can be reflected by altered characteristics electrocardiogram (ECG). Due to simplicity and non-invasive nature, ECG has been widely used for detecting arrhythmias there an urgent need automatic detection. Up date, some algorithms have proposed classification cardiac based on features ECG; however, their stratification rate still poor due unreliable signal or limited generalization capability classifier,...
Treatment of HeLa cells with a DNA topoisomerase I-specific inhibitor, camptothecin, results in rapid cessation the synthesis 45S rRNA precursor. The inhibition is reversible following drug removal and correlates presence camptothecin-trapped I-DNA abortive complexes, which can be detected as I-linked breaks upon lysis sodium dodecyl sulfate. These were found to concentrated within transcribed region human genes. No such sites inactive genes mouse-human hybrid cells, suggesting preferential...
Investigating the mechanisms underlying genesis and conduction of electrical excitation in atria at physiological pathological states is great importance. To provide knowledge concerning excitation, we constructed a biophysical detailed anatomically accurate computer model human that incorporates both structural electrophysiological heterogeneities. The three-dimensional geometry was extracted from visible female dataset. sinoatrial node (SAN) atrium, including crista terminalis (CT),...
A chloramphenicol resistance gene was cloned into a plasmid-borne dut gene, producing an insertion mutation that then transferred to the chromosome by allelic exchange. The could not be acquired haploid strains through substitutive recombination, even when two flanking markers were simultaneously transduced. easily transferred, via generalized transduction, chromosomal region of harboring lambda + transducing phage; however, resulting mutant/lambda merodiploid cured prophage. This apparent...
Early development of ionic models for cardiac myocytes, from the pioneering modification Hodgkin–Huxley giant squid axon model by Noble to iconic DiFrancesco–Noble integrating voltage-gated currents, ion pumps and exchangers, Ca 2+ sequestration -induced release, provided a general description mammalian Purkinje fibre (PF) framework modern models. In past two decades, has focused on tissue-specific with an emphasis sino-atrial (SA) node, atria ventricles, while PFs have largely been...
Chronic atrial fibrillation (AF) is associated with structural and electrical remodelling in the atria, which are a high recurrence of AF. Through biophysically detailed computer modelling, this study investigated mechanisms by AF-induced promotes perpetuates A family Courtemanche-Ramirez-Nattel variant models human cell action potentials (APs), taking into account intrinsic electrophysiological properties, was modified to incorporate various experimental data sets on changes major ionic...
Atrial fibrillation (AF) is the most common cardiac arrhythmias causing morbidity and mortality. AF may appear as episodes of very short (i.e., proximal AF) or sustained duration persistent AF), either form which causes irregular ventricular excitations that affect global function heart. It an unmet challenge for early automatic detection AF, limiting efficient treatment strategies AF. In this study, we developed a new method based on continuous wavelet transform 2D convolutional neural...
Abstract Cardiac arrhythmias and conduction disturbances are accompanied by structural remodelling of the specialised cardiomyocytes known collectively as cardiac system. Here, using contrast enhanced micro-computed tomography, we present, in attitudinally appropriate fashion, first 3-dimensional representations system within intact human heart. We show that cardiomyocyte orientation can be extracted from these datasets at spatial resolutions approaching single cell. These data commonly...
Micro-computed tomography (micro-CT) has been widely used to generate high-resolution 3-D tissue images from small animals nondestructively, especially for mineralized skeletal tissues. However, its application the analysis of soft cardiovascular tissues limited by poor inter-tissue contrast. Recent ex vivo studies have shown that contrast between muscular and connective in micro-CT can be enhanced staining with iodine. In present study, we apply this novel technique imaging structures...
Abstract The sinus node is a collection of highly specialised cells constituting the heart’s pacemaker. molecular underpinnings its pacemaking abilities are debated. Using high-resolution mass spectrometry, we here quantify >7,000 proteins from and neighbouring atrial muscle. Abundances 575 differ between two tissues. By performing single-nucleus RNA sequencing biopsies, attribute measured protein abundances to specific cell types. data reveal significant differences in ion channels...
The dynamical fluctuations in the rhythms of biological systems provide valuable information about underlying functioning these systems. During past few decades analysis cardiac function based on heart rate variability (HRV; variation R wave to intervals) has attracted great attention, resulting more than 17000-publications (PubMed list). However, it is still controversial underling mechanisms HRV. In this study, we performed both linear (time domain and frequency domain) nonlinear HRV data...
Abstract It is highly debated how cyclic adenosine monophosphate-dependent regulation (CDR) of the major pacemaker channel HCN4 in sinoatrial node (SAN) involved heart rate by autonomic nervous system. We addressed this question using a knockin mouse line expressing monophosphate-insensitive channels. This displayed complex cardiac phenotype characterized sinus dysrhythmia, severe bradycardia, pauses and chronotropic incompetence. Furthermore, absence CDR leads to inappropriately enhanced...
BackgroundHeart rate follows a diurnal variation, and slow heart rhythms occur primarily at night.ObjectiveThe lower during sleep is assumed to be neural in origin, but here we tested whether day-night difference intrinsic pacemaking involved.MethodsIn vivo vitro electrocardiographic recordings, vagotomy, transgenics, quantitative polymerase chain reaction, Western blotting, immunohistochemistry, patch clamp, reporter bioluminescence chromatin immunoprecipitation were used.ResultsThe the...
We have examined sino-atrial node (SAN) function in hearts from adult mice with heterozygous targeted disruption of the Scn5a gene to clarify role Scn5a-encoded cardiac Na+ channels normal SAN and mechanism(s) by which reduced channel might cause sinus dysfunction. Scn5a+/- showed depressed heart rates occasional (SA) block. Their isolated peripheral pacemaker cells a expression slowed intrinsic rates. Wild-type (WT) preparations exhibited similar activation patterns but significantly slower...
Because of its complexity, the atrioventricular node (AVN), remains 1 least understood regions heart. The aim study was to construct a detailed anatomic model AVN and relate it function. electric activity rabbit preparation imaged using voltage-dependent dye. then fixed sectioned. Sixty-five sections at 60- 340-microm intervals were stained for histology immunolabeled neurofilament (marker nodal tissue) connexin43 (gap junction protein). This revealed multiple structures within around AVN,...
Abstract We have previously shown in rabbit that the pacemaker of heart (the sinus node) is widespread and matches wide distribution leading site within right atrium. There is, however, uncertainty about precise location human heart, its spatial relationships with surrounding atrial muscle. Therefore, aim current study was to investigate node tissue a series healthy hearts and, for one construct computer three‐dimensional anatomical model node, including likely orientation myocytes. A...
Background— Computer models that capture key features of the heterogeneous myofiber architecture right and left atria interatrial septum provide a means investigating mechanisms responsible for atrial arrhythmia. The data necessary to implement such have not previously been available. aims this study were characterize surface geometry throughout chambers investigate effects structure on activation. Methods Results— Atrial orientations reconstructed in 3D at 50×50×50-μm 3 resolution from...
Mutations in the cardiac Na(+) channel gene (SCN5A) can adversely affect electric function heart, but effects be age dependent. We explored interacting of Scn5a disruption and aging on pathogenesis sinus node dysfunction a heterozygous knockout (Scn5a(+/-)) mouse model.We compared functional, histological, molecular features young (3 to 4 month) old (1 year) wild type Scn5a(+/-) mice. Both were associated with decreased heart rate variability, reduced sinoatrial automaticity, slowed...