A5030188411- Bioinformatics and Genomic Networks
- Chronic Kidney Disease and Diabetes
- Genomics and Phylogenetic Studies
- RNA modifications and cancer
- Microbial infections and disease research
- Bacterial biofilms and quorum sensing
- Viral Infectious Diseases and Gene Expression in Insects
- Molecular Biology Techniques and Applications
- Gene expression and cancer classification
- Microbial Metabolic Engineering and Bioproduction
- Gene Regulatory Network Analysis
- Microbial Community Ecology and Physiology
- Gut microbiota and health
- CRISPR and Genetic Engineering
- DNA Repair Mechanisms
- Animal Genetics and Reproduction
- Acute Kidney Injury Research
- Gastrointestinal Tumor Research and Treatment
- Protein purification and stability
- Turtle Biology and Conservation
- Machine Learning in Bioinformatics
- Veterinary Oncology Research
- Nanoparticles: synthesis and applications
- Chronic Disease Management Strategies
- Viral-associated cancers and disorders
North Carolina Agricultural and Technical State University
2014-2024
Michigan State University
1998-2020
Carolinas Medical Center
2017
Carolinas Healthcare System
2017
Wayne State University
2010
Andover Eye Associates
2008-2010
Center for Systems Biology
2008
Indiana University – Purdue University Indianapolis
2008
Clark Atlanta University
1998
The University of Queensland
1995
More detailed sequence standards that keep up with revolutionary sequencing technologies will aid the research community in evaluating data.
The recent exponential increase in the use of engineered nanoparticles (eNPs) means both greater intentional and unintentional exposure eNPs to microbes. Intentional includes as biocides. Unintentional results from fact that are included a variety commercial products (paints, sunscreens, cosmetics). Many these composed heavy metals or metal oxides such silver, gold, zinc, titanium dioxide, zinc oxide. It is thought since metallic/metallic oxide NPs impact so many aspects bacterial physiology...
Although pooled-population sequencing has become a widely used approach for estimating allele frequencies, most work proceeded in the absence of proper statistical framework. We introduce self-sufficient, closed-form, maximum-likelihood estimator frequencies that accounts errors associated with sequencing, and likelihood-ratio test statistic provides simple means evaluating null hypothesis monomorphism. Unbiased estimates (where N is number individuals sampled) appear to be unachievable,...
Using the 3T3-F442A preadipocyte line as a model of GH-dependent differentiation, early changes in DNA-binding affinity transcription factors response to GH addition were investigated. Addition 50 ng/ml human cells chemically defined medium led rapid increase binding activity activator protein 1 (AP-1) and CCAAT enhancer-binding (C/EBP), which was significant at 30 min reached maximal induction by 2 h (3-fold for AP-1, 2.5-fold C/EBP). Induction AP-1 DNA correlates with concomitant...
This study evaluated neoplasia in fish using medical records from zoos, aquariums, and exotic animal veterinarians. The parameters included geographic location, habitat type, signalment, anatomic location of neoplasia, type as confirmed with histologic examination, survival time, treatments provided for each patient. These data were entered into the Exotic Species Cancer Research Alliance (ESCRA) database. Out 455 cases across United States England, most animals submitted zoologic parks or...
The modeling of genetic interactions within a cell is crucial for basic understanding physiology and applied areas such as drug design. Interactions in gene regulatory networks (GRNs) include effects transcription factors, repressors, small metabolites, microRNA species. In addition, the are not always simultaneous, but can occur after finite time delay, or combined outcome simultaneous delayed interactions. Powerful biotechnologies have been rapidly successfully measuring levels expression...
Using an adaptive strategy, Chinese hamster ovary (CHO) cell lines were developed that are capable of robust growth in serum-free suspension culture. These preadapted derivatives the commonly used strain CHO cells (CHO DUKX), termed PA-DUKX, for introduction and stable expression several heterologous human genes. A significant advantage recombinant PA-DUKX was their ability to readily resume culture after transfection amplification Expression proteins quantitatively similar lineages...
Cells without intact mitochondrial DNA have been shown to lack the bystander effect, which is an energy-dependent process. We hypothesized that cells harboring mutations in genes responsible for ATP synthesis would show a decreased effect compared normal cells. Radiation-induced effects were analyzed two and four mutant human lymphoblastoid Medium from previously irradiated (conditioned medium) was transferred unirradiated respective cell lines evaluated using cytokinesis-block micronucleus...
Metallic antimicrobial materials are of growing interest due to their potential control pathogenic and multidrug-resistant bacteria. Yet we do not know if utilizing these can lead genetic adaptations that produce even more dangerous bacterial varieties.Here utilize experimental evolution strains Escherichia coli K-12 MG1655 resistant to, the iron analog, gallium nitrate (Ga(NO3)3). Whole genome sequencing was utilized determine genomic changes associated with resistance. Computational...
Abstract Background New systems biology studies require researchers to understand how interplay among myriads of biomolecular entities is orchestrated in order achieve high-level cellular and physiological functions. Many software tools have been developed the past decade help visually navigate large networks interactions with built-in template-based query capabilities. To further advance researchers' ability interrogate global states cells through multi-scale visual network explorations,...
With the introduction of increasingly powerful mass spectrometry (MS) techniques for clinical research, several recent large-scale MS proteomics studies have sought to characterize entire human plasma proteome with a general objective identifying thousands proteins leaked from tissues in circulating blood. Understanding basic constituents, diversity, and variability is essential development sensitive molecular diagnosis treatment monitoring solutions future biomedical applications....
Tuberculosis (TB) is a serious health concern, particularly in developing countries. Various delays, such as patient delay (PD) and healthcare system (HSD) the TB process, are exacerbating disease burden increasing rates of transmission mortality various global communities. Therefore, aim this study to identify risk factors associated with PD HSD patients Tabriz, Iran. A cross-sectional was conducted on 173 Iran from 2012 2014. Patients were interviewed semi-structured questionnaire....
Neoplasia due to environmental contaminants has been previously reported in wild amphibians; however, minimal research done investigate individual cases of amphibian neoplasia independent contamination or viral etiologies. This study reviewed published literature for amphibians order supplement the Exotic Species Cancer Research Alliance (ESCRA) database. Through searches three scientific databases (PubMed, CAB Abstracts, and Web Science Zoological Record), 50 were found 36 articles between...
Abstract Background There has been an increased usage of metallic antimicrobial materials to control pathogenic and multi-drug resistant bacteria. Yet, there is a corresponding need know if this leads genetic adaptations that could produce more harmful strains. Methodology Experimental evolution was used adapt Escherichia coli K-12 MG1655 excess iron (II) with subsequent genomic analysis. Phenotypic assays gene expression studies were conducted demonstrate pleiotropic effects associated...
Background: Toxoplasma gondii (T. gondii) is a ubiquitous obligatory intracellular parasite which infects over 40 million Americans and causes toxoplasmosis. Inside the human body, T. can damage tissues invade vital organs. Methods: This study evaluated association of infection liver disease using data from National Health Nutrition Examination Survey (NHANES) 2009–2010, with sample size 3371 participants (age 20–80 years). was determined by level IgG antibody in serum samples. Liver...
Gene regulatory networks represent an abstract mapping of gene regulations in living cells. They aim to capture dependencies among molecular entities such as transcription factors, proteins and metabolites. In most applications, the network structure is unknown, has be reverse engineered from experimental data consisting expression levels genes usually measured messenger RNA concentrations microarray experiments. Steady-state are obtained measurements variations activity following...
This multi-institutional collaborative study of neoplasia in snakes reviewed medical records at each facility to determine species prevalence, survival, and methods treatment. Complete numbers were also collected facility. In total, 65 species, 133 snakes, 149 unique neoplasias included this study. Affected age, sex, their tumor type location, metastasis, treatment, survival data are reported. The highest species-specific prevalence was Common or Northern Watersnakes (Nerodia sipedon)...
Abstract A central focus of clinical proteomics is to search for biomarkers in plasma diagnostic and therapeutic use. We studied a set proteins accessed from the Healthy Human Individual's Integrated Plasma Proteome (HIP 2 ) database, larger curated human proteins, subset inflammatory overlap with sets known protein biomarkers, drug targets, secreted proteins. Most were found occur plasma, over three times level their interacting neighbors compared Percentage overlaps Gene Ontology terms...
A majority of antibiotic-resistant bacterial infections in the United States are associated with biofilms. Nanoscale biophysical measures increasingly revealing that adhesive and viscoelastic properties bacteria play essential roles across multiple stages biofilm development. Atomic Force Microscopy (AFM) applied to strains variation antimicrobial resistance enables new opportunities for investigating function forces (stickiness) formation. AFM force spectroscopy analysis a field strain...
African Americans are disproportionately burdened by diabetic kidney disease (DKD). However, little is known about the cellular and molecular mechanisms underlying onset progression of DKD in this population. The goal current study was to determine association between specific inflammation markers injury American men. To end, we recruited patients either with (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mi>n</mml:mi><mml:mo>=</mml:mo><mml:mn>20</mml:mn></mml:math>)...
Wildlife Safari, a zoo located in Winston, Oregon, has fed donated carcass meat as diet to carnivores for over 30 yr. Carcass is an alternative commercially prepared meat. Donated arrives at Safari entire animal. Cattle (Bos taurus), horse (Equus caballus), deer (Odocoileus hemionus), and elk (Cervus elaphus roosevelti) have been donated. Bacterial testing was performed on site with the use of Neogen Reveal immunosorbent assays. Testing focused Salmonella spp., Listeria Escherichia coli...