A5101765042- Reproductive System and Pregnancy
- Circular RNAs in diseases
- Genetic and phenotypic traits in livestock
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Endometriosis Research and Treatment
- Reproductive Physiology in Livestock
- Reproductive Biology and Fertility
- RNA Research and Splicing
- Microbial Metabolism and Applications
- Mercury impact and mitigation studies
- RNA modifications and cancer
- Hypothalamic control of reproductive hormones
- Antimicrobial Peptides and Activities
- Biochemical and Structural Characterization
- Silkworms and Sericulture Research
- Selenium in Biological Systems
- Cytokine Signaling Pathways and Interactions
- Salivary Gland Disorders and Functions
- Wnt/β-catenin signaling in development and cancer
- Cyclone Separators and Fluid Dynamics
- Growth Hormone and Insulin-like Growth Factors
- Gynecological conditions and treatments
- Iron Metabolism and Disorders
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
Northwest A&F University
2010-2022
Agriculture and Forestry University
2021
Chinese Academy of Agricultural Sciences
2017
Institute of Animal Sciences
2017
Yangzhou University
2013
Hainan Provincial Academy of Agricultural Sciences
2009
Changes in endometrial cell morphology and function are absolutely necessary for successful embryo implantation. In this study, miR‐26a was widely expressed dairy goats, found to be regulated by β‐estradiol (E2) progesterone (P4) epithelium cells (EECs) as well stromal (ESCs). Furthermore, played a role the regulation of proliferation apoptosis directly regulating PTEN indirectly PI3K/AKT pathway EECs but not ESCs goats vitro. addition, expression osteopontin (OPN), vascular endothelial...
Abstract Endometrium receptivity is essential for successful embryo implantation in mammals. However, the lack of genetic information remains an obstacle to understanding mechanisms underlying development a receptive endometrium from pre-receptive phase dairy goats. In this study, more than 4 billion high-quality reads were generated and de novo assembled into 102,441 unigenes; these unigenes annotated using published databases. A total 3,255 that differentially expressed (DEGs) between PE...
Circular RNAs (circRNAs) have been found to play important functional roles in epigenetic regulation under certain physiological and pathological conditions. However, knowledge of circRNAs during the development receptive endometrium (RE) from pre-RE is limited. In RE dairy goats, higher circRNA-9119 levels, with lower miR-26a prostaglandin-endoperoxide synthase 2 (PTGS2) were detected. Further study showed that decreased levels by acting as a microRNA sponge, downregulated expression PTGS2...
Recent studies have revealed that noncoding RNAs play important regulatory roles in the formation of endometrial receptivity. Circular (circRNAs) are a universally expressed RNA species been recently proposed to act as miRNA sponges directly regulate expression target genes or parental genes.
Development of the receptive endometrium (RE) from pre-receptive (PE) is essential for embryo implantation, but its molecular mechanisms have not been fully understood. In this study, lncRNA-miRNA-mRNA and circRNA-miRNA-mRNA networks were constructed to explore functions potential competing endogenous RNAs (ceRNA) during development RE in dairy goats. We observed that circRNA8073 (ciR8073) decreased levels miR-181a by acting as a miRNA sponge. This effect indirectly increased expression...
Abstract microRNAs (miRNAs) and circular RNAs (circRNAs) are important for endometrial receptivity establishment embryo implantation in mammals. miR‐34a miR‐34c highly expressed caprine receptive endometrium (RE). Herein, the functions mechanisms of miR‐34a/c epithelial cell (CEEC) apoptosis RE were investigated. downregulated expression level centrosomal protein 55 ( CEP55 ) sponged by circRNA8073 (circ‐8073), thereby exhibiting a negative interaction CEEC. induced CEEC targeting...
The development of the udder and milk yield are closely related to number vitality mammary epithelial cells. Many previous studies have proved that non-coding RNAs (ncRNAs) widely involved in gland physiological activities lactation. Our laboratory sequencing data revealed miR-574-5p was differentially expressed during colostrum peak lactation stages, while molecular mechanism regulatory effect on goat cells (GMECs) is unclear. In this study, targeting relationship detected between or...
Abstract The growth of endometrial stromal cells (ESCs) at implantation sites may be a potential factor affecting the success rate embryo implantation. Incremental proofs demonstrated that ncRNAs (e.g. miRNAs, lncRNAs and circRNAs) were involved in various biological procedures, including proliferation apoptosis. In this study, role miR‐100‐5p on apoptosis goat ESCs vitro vivo was determined. mRNA expression significantly inhibited receptive phase (RE) rather than pre‐receptive (PE)....
Increasing evidence has shown that miRNAs play important roles in endometrium development during the menstrual cycle humans and many other animals. Our previous data indicated miR-182 levels increase 15.55-fold pleiotrophin (PTN) decrease 20.97-fold receptive (RE, D15) compared with pre-receptive (PE, D5) dairy goats. The present study shows is widely expressed different tissues of goats its expression are regulated by E2 P4 endometrial epithelium cells (EECs). We confirmed PTN a target...
In this study, an RT-qPCR analysis showed that the expression levels of miR-449a in pre-receptive endometrium were lower compared to receptive endometrium, which is consistent with previous sequencing data (previous investigations). To detect role endometrial receptivity, we transfected caprine stromal cells (ESCs) cultured vitro mimics. The results revealed decreased mRNA and protein LGR4 by targeting its 3'-untranslated region. mimics significantly reduced G1 cell population from 52.56%...
We investigated the polymorphisms of PRLR and FOLR1 genes in Xinong Saanen, Guanzhong, Boer goat breeds by DNA sequencing PCR-RFLP.Two novel SNPs were identified: KC109741: g.62130C>T 3ꞌ-UTR gene PRLR, KC136296: g.7884A>C exon 3 FOLR1.In three breeds, polymorphism information content was 0.20-0.27at locus.At locus, it 0.36 goats.The Hardy-Weinberg disequilibrium at locus.The SNP found to be significantly associated with milk production traits Saanen Guanzhong breeds.These results are...
The endometrium undergoes a series of complex changes to form receptive (RE) that allows the embryo be implanted. inability establish endometrial receptivity livestock causes implantation failure and considerable losses animal husbandry. MicroRNAs (miRNAs) are class noncoding RNAs. Studies have found miRNAs can regulate many critical physiological processes, including establishment RE during implantation. miR-184 is highly expressed in period dairy goats. This study aimed explore effect on...
Circular RNAs (circRNAs), which are considered a large class of endogenous noncoding RNAs, function as regulators in various biological procedures. In this study, the and molecular mechanisms circRNA8220 goat mammary epithelial cells (GMECs) were explored. CircRNA8220 could spong miR-8516 block by binding to target site negative feedback relationship existed between miR-8516. Stanniocalcin 2 (STC2) was gene up-regulate expression STC2 sponging GMECs. circRNA8220/miR-8516/STC2 promote...
Abstract Despite the fact that long noncoding RNAs (lncRNAs) play roles in almost all biological processes, little is known about their function endometrium during formation of endometrial receptivity. In this study, a comprehensive analysis lncRNAs goat tissues on Day 5 (prereceptive endometrium, PE) and 15 (receptive RE) pregnancy was performed by using RNA‐Seq. As result, 668 differentially expressed (DELs) were found between PE RE. Further study showed lncRNA882, regulated estrogen (E2)...
Polymorphisms of DGAT1 and STAT5A genes in Xinong Saanen Guanzhong goat breeds were investigated. PCR-RFLP, SSCP, DNA sequencing used to identify three SNPs: DQ380250:g.407_408insC the gene, AJ237937:g.6798C>T g.6852C>T gene. In g.407_408insC locus, frequencies C- allele 0.79-0.85, C+ 0.21-0.15. At C 0.70-0.72, T 0.30-0.28. Compared with goats C-C-, those C-C+ genotype had greater milk fat (P < 0.05). The CT yield than CC results showed that does C-C-CT C-C+CT yielded more C-C-CC(P addition,...
Circular RNAs (circRNAs) in various tissues and cell types from mammalian sources have been studied. However, present knowledge on circRNAs the development of pre‐receptive endometrium (PE) dairy goats is limited. In goats, higher circRNA3175 (ciR3175) levels, lower miR‐182 levels Testin ( TES ) were detected. And ciR3175 could decreased by acting as a miRNA sponge, down‐regulated expression level via predicted target site endometrial epithelial cells (EECs) vitro. Via this way, functioned...