A5089711092- Glycosylation and Glycoproteins Research
- Pancreatic and Hepatic Oncology Research
- Galectins and Cancer Biology
- Spaceflight effects on biology
- Cancer Research and Treatments
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Monoclonal and Polyclonal Antibodies Research
- Bone health and osteoporosis research
- Signaling Pathways in Disease
- Radiopharmaceutical Chemistry and Applications
- Proteoglycans and glycosaminoglycans research
- Pancreatitis Pathology and Treatment
- Platelet Disorders and Treatments
- Hematopoietic Stem Cell Transplantation
- Carbohydrate Chemistry and Synthesis
- Prostate Cancer Treatment and Research
- Cancer, Hypoxia, and Metabolism
- Cancer Genomics and Diagnostics
- Space Exploration and Technology
- Epigenetics and DNA Methylation
- Medical Imaging Techniques and Applications
- Caveolin-1 and cellular processes
- MicroRNA in disease regulation
- Fibroblast Growth Factor Research
- Gene expression and cancer classification
Michigan State University
2024-2025
Molecular Theranostics (United States)
2025
Grand Rapids Women's Health
2024
Van Andel Institute
2011-2022
Indiana University School of Medicine
2017-2022
Indiana University – Purdue University Indianapolis
2017-2022
Indiana University
2019-2021
Background The ability to selectively detect and target cancer cells that have undergone an epithelial-mesenchymal transition (EMT) may lead improved methods treat cancers such as pancreatic cancer. remodeling of cellular glycosylation previously has been associated with cell differentiation represent a valuable class molecular targets for EMT. Methodology/Principal Findings As first step toward investigating the nature alterations in EMT, we characterized expression glycan-related genes...
Glycan-binding proteins are commonly used as analytical reagents to detect the levels of specific glycan structures in biological samples. A detailed knowledge specificities glycan-binding is required for properly interpreting their binding data. powerful technology characterizing specificity array. However, interpretation glycan-array data can be difficult due complex fine certain proteins. We developed a systematic approach, called outlier-motif analysis, extracting fine-specificity...
The CA 19-9 assay detects a carbohydrate antigen on multiple protein carriers, some of which may be preferential carriers the in cancer. We tested hypothesis that measurement individual proteins could improve performance over standard assay. used antibody arrays to measure levels serum or plasma samples from patients with pancreatic adenocarcinoma pancreatitis. Sample sets three different institutions were examined, comprising 531 samples. any did not upon (82% sensitivity at 75% specificity...
The current best serum marker for pancreatic cancer, CA 19-9, detects a carbohydrate antigen on multiple protein carriers. Better knowledge of the carriers 19-9 in various disease states may lead to improved diagnostic tests. To identify proteins that carry antigen, we immunoprecipitated from pooled sera and identified associated using MS. Among high-confidence identifications, confirmed presence Apolipoprotein B-100 by antibody arrays Western blot kininogen, ARVCF, E arrays. We...
Nonagenarians with metastatic castration-resistant prostate cancer (mCRPC) are often underrepresented in research due to their limited numbers among treated populations. For this group, traditional chemotherapy carries significant side effects that can severely impact quality of life. Targeted therapy designed selectively bind cells expressing the prostate-specific membrane antigen (PSMA) presents a promising alternative, particularly for those widespread disease and contraindications...
The CA 19-9 antigen is currently the best individual marker for detection of pancreatic cancer. In order to optimize assay and develop approaches further improve cancer detection, it important understand specificity differences between antibodies consequential affect on biomarker performance. Antibody arrays enabled multiplexed comparisons five different used in analysis plasma samples from patients controls. Major were observed their particular patient samples. Glycan array revealed that...
Abstract Gravity has an important role in both the development and maintenance of bone mass. This is most evident rapid intense loss observed humans animals exposed to extended periods microgravity spaceflight. Here, cohabitating 9-week-old male C57BL/6 mice resided spaceflight for ~4 weeks. A skeletal survey these was compared habitat matched ground controls determine effects baseline samples order maturation on resulting phenotype. We hypothesized that weight-bearing bones would experience...
Abstract Spaceflight results in reduced mechanical loading of the skeleton, which leads to dramatic bone loss. Low mass is associated with increased fracture risk, and this combination may compromise future, long-term, spaceflight missions. Here, we examined systemic effects surgery/healing on several non-injured bones within axial appendicular skeleton. Forty C57BL/6, male mice were randomized into following groups: (1) Sham surgery housed earth (Ground + Sham); (2) Femoral segmental defect...
Specific protein glycoforms may be uniquely informative about the pathological state of a cyst and serve as accurate biomarkers. Here we tested that hypothesis using antibody-lectin sandwich arrays in broad screens targeted studies candidate markers. We profiled 16 different proteins captured by 72 antibodies fluid from mucinous nonmucinous cysts (n = 22), then three-marker panel 22 addition samples blinded samples. Glycan alterations were not widespread among mainly confined to MUC5AC...
In academia, authorship is considered a currency and important for career advancement. As the Journal of Bone Mineral Research (JBMR) highest-ranked journal in field bone, muscle, mineral metabolism official publication American Society Research, we sought to examine changes over JBMR's 30-year history. Two bibliometric methods were used collect data. The "decade method" included all published manuscripts throughout 1 year each decade past 30 years starting with inaugural year, yielding 746...
Schistosome parasites follow a complex migration path through various tissues, changing their antigenic profile as they develop. A thorough understanding of the antibody response in each tissue region could help unravel immunology these developing and aid vaccine design. Here we used novel strategy for analysing local responses induced by Schistosoma japonicum infection at site infection. Cells from rat lymph nodes draining sites larval (the skin lungs), liver‐lymph where adults reside...
Emerging evidence demonstrates that megakaryocytes (MK) play key roles in regulating skeletal homeostasis and hematopoiesis. To test if the loss of MK negatively impacts osteoblastogenesis hematopoiesis, we generated conditional knockout mice where Mpl, receptor for main growth factor, thrombopoietin, was deleted specifically (Mplf/f;PF4cre). Unexpectedly, at 12 weeks age, these exhibited a 10-fold increase platelets, significant expansion hematopoietic/mesenchymal precursors, remarkable...
Imbalances between bone formation and resorption, which can occur due to aging or sex hormone deprivation, result in decreased mass an increased risk of fracture. Previous studies have suggested that the β-galactoside binding lectin, galectin-3, is involved remodeling. We compared parameters mice having null alleles galectin-3 gene (Lgals3-KO) with those their wild-type littermates. Lgals3 deficiency cortical expansion at 36 weeks (wk) preserved enhanced both male female mutant mice. In...
Abstract With increased human presence in space, bone loss and fractures will occur. Thrombopoietin (TPO) is a recently patented healing agent. Here, we investigated the systemic effects of TPO on mice subjected to spaceflight sustaining fracture. Forty, 9-week-old, male, C57BL/6 J were divided into 4 groups: (1) Saline+Earth; (2) + Earth; (3) Saline+Flight; (4) Flight ( n = 10/group). Saline- TPO-treated underwent femoral defect surgery, 20 housed space (“Flight”) Earth for approximately...
Megakaryocytes (MKs) support bone formation by stimulating osteoblasts (OBs) and inhibiting osteoclasts (OCs).Aging results in higher resorption, leading to loss.Whereas previous studies showed the effects of aging on MK-mediated formation, OC is poorly understood.Here we examined effect thrombopoietin (TPO) MK-derived conditioned media (CM) from young (3-4 months) aged (22-25 mice precursors.Our findings that significantly increased vitro.Moreover, expression TPO receptor, Mpl, circulating...
The development of accurate clinical biomarkers has been challenging in part due to the diversity between patients and diseases. One approach account for is use multiple markers classify patients, based on concept that each individual marker contributes information from its respective subclass patients. Here we present a new strategy developing biomarker panels accounts completely distinct patient subclasses. Marker State Space (MSS) defines "marker states" all possible patterns high low...
Significant efforts are underway to develop new biomarkers from pancreatic cyst fluid. Previous research has made use of fluid collected surgically removed cysts, but the clinical implementation would by endoscopic ultrasound-guided, fine-needle aspiration (EUS-FNA). The purpose this study was investigate applicability obtained using surgical specimens. Matched pairs operating-room (OR) and EUS-FNA samples 12 patients were evaluated for levels three previously described biomarkers, CA 19-9,...
γ-Glutamyl transpeptidase 1 (GGT1) is an N-glycosylated membrane protein that catabolizes extracellular glutathione and other γ-glutamyl-containing substrates. In a variety of disease states, including tumor formation, the enzyme shed from surface cell can be detected in serum. The structures N-glycans on human GGT1 (hGGT1) have been shown to tissue-specific. Tumor-specific changes glycans also observed, suggesting hGGT1 would important biomarker for detecting tumors monitoring their...
The sixth annual Origins of Cancer Symposium was held July 10, 2015, at the Van Andel Research Institute in Grand Rapids, MI.Its theme Beyond Genome, with talks focused on various influences posttranslational modifications malignant transformation and spread cancer.This event organized by senior Ph.D. students as part their professional development training Graduate School is a successor to original Oncogene meetings established Foundation for Advanced Studies 1980s.The symposium featured...