A5008074896- Trace Elements in Health
- RNA Research and Splicing
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- RNA and protein synthesis mechanisms
- Genomics and Chromatin Dynamics
- Fungal and yeast genetics research
- Rabbits: Nutrition, Reproduction, Health
- Animal Nutrition and Physiology
- Erythrocyte Function and Pathophysiology
- Microbial bioremediation and biosurfactants
- Digestive system and related health
- Aluminum toxicity and tolerance in plants and animals
- Ruminant Nutrition and Digestive Physiology
- COVID-19 Clinical Research Studies
- Respiratory viral infections research
- Vibrio bacteria research studies
- Mitochondrial Function and Pathology
- Microscopic Colitis
- Celiac Disease Research and Management
- Clostridium difficile and Clostridium perfringens research
- Genetic Neurodegenerative Diseases
- Monoclonal and Polyclonal Antibodies Research
- Biosensors and Analytical Detection
- Alkaline Phosphatase Research Studies
Clemson University
2022-2025
Illumina (United States)
2024
King's College London
2023
The Ohio State University
2016-2020
Laboratory of Molecular Genetics
2017
National Institute of Technology Calicut
2016
University of Southern California
1989
Background Higher viral loads in SARS-CoV-2 infections may be linked to more rapid spread of emerging variants concern (VOC). Rapid detection and isolation cases with highest loads, even pre- or asymptomatic individuals, is essential for the mitigation community outbreaks. Methods findings In this study, we analyze Ct values from 1297 positive patient saliva samples collected at Clemson University testing lab upstate South Carolina. Samples were identified as using RT-qPCR, clade information...
Background The prevalence and severity of duodenal injury in coeliac disease patients controlled on a gluten-free diet is unclear. Aims To use quantitative histology to assess treated disease. Methods Quantitative pre-treatment biopsies collected clinical trials assessing an investigational immunotherapy for was analysed. Morphometric readings were converted Marsh classifications. Results Ninety-three had biopsies. For well-oriented sections second part biopsies, six (6%) classified as 0 or...
Glycolysis and the pentose phosphate pathway both play a central role in degradation of glucose all domains life. Another metabolic route that can facilitate breakdown is gluconate shunt. In this shunt dehydrogenase kinase catalyze two-step conversion into intermediate 6-phosphogluconate. Despite presence these enzymes many organisms, their only established production 6-phosphogluconate for Entner-Doudoroff pathway. report we performed profiling on strain Schizosaccharomyces pombe lacking...
Abstract Background Higher viral loads in SARS-CoV-2 infections may be linked to more rapid spread of emerging variants concern (VOC). Rapid detection and isolation cases with highest loads, even pre- or asymptomatic individuals, is essential for the mitigation community outbreaks. Methods Findings In this study, we analyze Ct values from 1297 positive patient saliva samples collected at Clemson University testing lab upstate South Carolina. Samples were identified as using RT-qPCR, clade...
A 28-day battery cage study was conducted to test the efficacy of tannin-free grain sorghum varieties fed Cobb 500 male broiler chickens (n = 512) and challenged with Eimeria maxima (EM) Clostridium perfringens (CP). Birds were 1 8 treatments (corn, red/bronze, white/tan, or U.S. No. 2 sorghum) grouped by challenge method (challenged EM/CP unchallenged). On d 14, birds in group orally inoculated ∼5,000 oocysts EM, on 19, 20, 21, given a broth culture CP ∼108 CFU/mL once daily. three scored...
The transcription in vitro of eucaryotic tRNA genes by RNA polymerase III requires two factors, designated TFIIIB and TFIIIC. One the critical functions TFIIIC is that it interacts directly specifically with internal promoter elements these genes. We have partially purified Saccharomyces cerevisiae chromatography on Bio-Rex 70, DEAE-cellulose, phosphocellulose resins. A 150-kilodalton (kDa) DNA-binding polypeptide copurified activity. This 150-kDa protein coeluted activity after...
Infection with pathogenic free-living amoebae, including Naegleria fowleri,
Summary In Schizosaccharomyces pombe, the expression of zrt1 zinc uptake gene is tightly regulated by status. When intracellular levels are low, highly expressed. However, when high, transcription blocked in a manner that dependent upon factor Loz1. To gain additional insight into mechanism which Loz1 inhibits high zinc, we used RNA‐seq to identify Loz1‐regulated genes, and ChIP‐seq analyze recruitment target promoters. We find recruited promoters 27 genes also repressed Loz1‐dependent...
University spring break carries a two-pronged SARS-CoV-2 variant transmission risk. Circulating variants from universities can spread to destinations, and destinations surrounding communities. Therefore, it is critical implement surveillance testing strategies limit community before after mitigate virus facilitate safely returning in-person teaching.
A unique tRNA(Ser)-tRNA(Met) tandem gene arrangement was characterized previously from Schizosaccharomyces pombe. Three alleles exist in which a tRNA(Ser) is separated by 7 base pairs an initiator tRNA(Met) gene. Promotion of transcription occurs only within the gene, yielding dimeric precursor transcript. Using nuclease protection and gel retardation assays, we have analyzed how Saccharomyces cerevisiae RNA polymerase III factor C (TFIIIC) interacts with this template. The primary...
Abstract Testing was paramount in the management of COVID-19 pandemic. Our university established KCL TEST: a SARS-CoV-2 asymptomatic testing programme that enabled sensitive and accessible PCR RNA saliva. We performed 158,277 PCRs for our staff, students, their household contacts, free charge. average turnaround time 16h 37 mins from user registration to result delivery. TEST combined open-source automation house non-commercial reagents, which allows rapid deployment. Here we provide...