A5091417138- Enzyme Production and Characterization
- Polysaccharides and Plant Cell Walls
- Plant tissue culture and regeneration
- Biofuel production and bioconversion
- Plant-Microbe Interactions and Immunity
- Transgenic Plants and Applications
- Plant Pathogenic Bacteria Studies
- Enzyme Structure and Function
- Fungal and yeast genetics research
- Aldose Reductase and Taurine
- Animal Genetics and Reproduction
- Legume Nitrogen Fixing Symbiosis
- RNA and protein synthesis mechanisms
- Polyamine Metabolism and Applications
- Cassava research and cyanide
- Enzyme Catalysis and Immobilization
- Microbial Metabolites in Food Biotechnology
- CRISPR and Genetic Engineering
- Mycotoxins in Agriculture and Food
- Studies on Chitinases and Chitosanases
- Plant Gene Expression Analysis
- Phytase and its Applications
- GABA and Rice Research
- Catalysis for Biomass Conversion
- Plant Pathogens and Fungal Diseases
Carlsberg Laboratory
1996-2022
Norwegian Cancer Society
2005
The codon usage of a hybrid bacterial gene encoding thermostable (1,3-1,4)-beta-glucanase was modified to match that the barley isoenzyme EII gene. Both and unmodified genes were fused DNA segment high-pI alpha-amylase signal peptide downstream promoter. When introduced into aleurone protoplasts, with adapted directed synthesis heat stable (1,3-1,4)-beta-glucanase, whereas activity heterologous enzyme not detectable when protoplasts transfected In different expression plasmid, cloned...
Improved agricultural and industrial production organisms are required to meet the future global food demands minimize effects of climate change. A new resource for crop microbe improvement, designated FIND-IT (Fast Identification Nucleotide variants by droplet DigITal PCR), provides ultrafast identification isolation predetermined, targeted genetic in a screening cycle less than 10 days. Using large-scale sample pooling combination with digital PCR (ddPCR) greatly increases size...
In β-glucans those β-1,4 glycosidic bonds which are adjacent to β-1,3 cleaved by endo-1,3-1,4-β-glucanases (β-glucanases). Here, the relationship between structure and activity of β-glucanase Bacillus macerans is studied x-ray crystallography site-directed mutagenesis active site residues. Crystal analysis at 2.3-Å resolution reveals a jellyroll protein with deep channel harboring amino acid residues Trp101, Glu103, Asp105, Glu107 as in hybrid H(A16-M) (Keitel, T., Simon, O., Borriss, R.,...
The molecular basis for the absence of anthocyanins and proanthocyanidins in four independent sodium azide-induced ant18 mutants barley was examined by sequencing gene encoding dihydroflavonol 4-reductase these mutants. Sodium azide generated 21 base substitutions, which corresponds to 0.17% 12,704 nucleotides sequenced. Of 86% were nucleotide transitions, 14% transversions. A.T-->G.C pair transitions about 3 times more frequent than G.C-->A.T transitions. No deletions or mutation hot...
The relationship between enzyme stability and glycosylation was examined for two different Bacillus (1,3-1,4)-β-glucanases following expression of the corresponding genes in Escherichia coli Saccharomyces cerevisiae. Both secreted from yeast cells were glycosylated a pronounced difference type extent observed. Thermostability analysis enzymes their unglycosylated counterparts synthesized by E. disclosed substantially higher thermotolerance enzymes. At 70 °C half-life form B. macerans...
A small cysteine-rich protein, the function of which remains elusive, was discovered in exudate a Penicillium species. Crystal diffraction experiments conducted using in-house Cu Kalpha radiation and an R-AXIS IV++ imaging-plate detector yielded high-quality data to 1.4 A, with distinguishable anomalous signal from sulfur (DeltaF/F = 0.031). This used phase solve structure single set; 64-residue amino-acid sequence unambiguously determined electron density. It revealed globular all-beta...
Phytopathogenic Erwinia bacteria cause tissue maceration by secretion of pectinolytic enzymes such as pectate lyase (PL). Sequencing overlapping genomic fragments from carotovora subsp. atroseptica established the organization a 7.5 kbp region encoding PL isoenzymes. Two intergenic regions 656 and 645 bp separate three enzyme coding 1125 exhibiting approximately 80% positional identity. The promoters each genes contain segment with high homology to binding sequence E. chrysanthemi KdgR...
Hybrid (1–3,1–4)‐β‐glucanases which contain an N‐terminal region derived from the Bacillus amyloliquefaciens enzyme and a C‐terminal of closely related B, macerans may exhibit thermostability superior to both parental enzymes. A systematic series hybrid enzymes were constructed in order delineate amino acid residues that affect protein stability. with between one four for mature (1–3,1–4)‐β‐glucanase no significant changes biochemical characteristics as compared enzyme. However,...
Components of garlic (Allium sativum) can cause disruption microtubules, cell cycle arrest, and apoptosis in cancer cells. We show here that a water-soluble extract arrested MDA-MB-435 cells mitosis caused apoptosis. The proapoptotic BH3-only, bcl-2 family protein BimEL, which healthy be tightly sequestered to the microtubule-associated dynein motor complex, was modified after treatment. main effect on BimEL considerable increase phosphorylated form protein. This phosphorylation(s), probably...
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