A5062597107- Food composition and properties
- Enzyme Production and Characterization
- Microbial Metabolites in Food Biotechnology
- Plant tissue culture and regeneration
- Plant Genetic and Mutation Studies
- Legume Nitrogen Fixing Symbiosis
- Chromosomal and Genetic Variations
- Wheat and Barley Genetics and Pathology
- Plant pathogens and resistance mechanisms
- Plant-Microbe Interactions and Immunity
- Transgenic Plants and Applications
- Biofuel production and bioconversion
- Microbial Community Ecology and Physiology
- Soil Mechanics and Vehicle Dynamics
- Phytase and its Applications
- Plant Parasitism and Resistance
- Genomics and Phylogenetic Studies
- Photosynthetic Processes and Mechanisms
- Agricultural Engineering and Mechanization
Carlsberg Laboratory
2016-2022
Aarhus University
2010-2020
University of Copenhagen
2014
Abstract Background Starch is stored in higher plants as granules composed of semi-crystalline amylopectin and amorphous amylose. provide energy for the plant during dark periods germination seeds tubers. Dietary starch also a highly glycemic carbohydrate being degraded to glucose rapidly absorbed small intestine. But portion dietary starch, termed “resistant starch” (RS) escapes digestion reaches large intestine, where it fermented by colonic bacteria producing short chain fatty acids...
Cereal grain germination is central for plant early development, and efficient has a major role in crop propagation malting. Endosperm starch the prime energy reserve seedling establishment. In this study, it was hypothesized that optimized granule structure, not only endosperm content per se, important For purpose, wild-type (WT), specifically engineered degradable hyperphosphorylated (HP) more resistant amylose-only (AO) barley lines were used. The transgenics showed no severe phenotypes...
Improved agricultural and industrial production organisms are required to meet the future global food demands minimize effects of climate change. A new resource for crop microbe improvement, designated FIND-IT (Fast Identification Nucleotide variants by droplet DigITal PCR), provides ultrafast identification isolation predetermined, targeted genetic in a screening cycle less than 10 days. Using large-scale sample pooling combination with digital PCR (ddPCR) greatly increases size...
ABSTRACT The importance of cereal starch production worldwide cannot be overrated. However, the qualities and resulting values existing raw processed do not fully meet future demands for environmentally friendly renewable, advanced biomaterials, functional foods, biomedical additives. New approaches bioengineering are needed. In this review, we discuss from a combined universal bioresource point view. combination new biotechniques clean technology methods can implemented to replace, example,...
Amylose synthesis is strictly associated with activity of granule-bound starch synthase (GBSS) enzymes. Among several crops there are cultivars containing types either little or no amylose known as near-waxy waxy. This (near) amylose-free phenotype a single locus (waxy) which has been mapped to GBSS-type genes in different crops. Most waxy varieties result low expression GBSS gene. However, some where the enzymes expressed normally. For these types, nucleotide polymorphisms have hypothesized...
Starch is the most important source of calories for human nutrition and majority it produced by cereal farming. also used as a renewable raw material in range industrial sectors. It can be chemically modified to introduce new physicochemical properties. In this way starch adapted variety specific end-uses. Recombinant DNA technologies offers an alternative processing. The plant biosynthetic pathway manipulated design starches with novel structure improved technological future may reduce or...
Cloning of gene casettes and other DNA sequences into the conventional vectors for biolistic or Agrobacterium-mediated transformation is hampered by a limited amount unique restriction sites difficulties often encountered when ligating small single strand overhangs. These problems are obviated "The Uracil Specific Excision Reagent (USER™)" technology (New England Biolabs) which thus offers new very time-efficient method engineering big complex plasmids. By application USER™ system, we...
Abstract Nonhost resistance, a resistance of plant species against all nonadapted pathogens, is considered the most durable and efficient immune system in plants. To increase our understanding response barley plants to infection by powdery mildew, Blumeria graminis f. sp. tritici , we used quantitative proteomic analysis (LC-MS/MS). We compared two genotypes cultivar Golden Promise, wild type (WT) with overexpression phytoglobin (previously hemoglobin) class 1 (HO), which has previously been...
Novel crop improvement methodologies, including the exploitation of natural genetic variation, are urgently required to feed our rapidly growing human population in context global climate change. Here we describe a ‘Fast Identification Nucleotide variants by DigITal PCR’ (FIND-IT) method for rapid identification pre-targeted or rare alleles large genomic populations. Libraries 500,000 individuals can be screened and desired isolated within two weeks. FIND-IT is widely applicable mining...
This study showed how barley plants can be shifted in their response to isolates of the mildew pathogen Blumeria graminis with different host adaptation by overexpression phytoglobin gene HvHb1 . At early infection stages, overexpressing ( GPH b1) less papilla formation and more hypersensitive against both virulent avirulent compared wildtype WT ) plants. The shift was most pronounced a wheat‐adapted isolate B. f. sp. tritici ). later b1 infected (A6) leaf chlorosis plants, indicating...