A5088692433- Seed Germination and Physiology
- Plant Stress Responses and Tolerance
- Protein Tyrosine Phosphatases
- Plant Molecular Biology Research
- Advanced Proteomics Techniques and Applications
- Soybean genetics and cultivation
- Plant tissue culture and regeneration
- Plant responses to water stress
- Photosynthetic Processes and Mechanisms
- Genetic Mapping and Diversity in Plants and Animals
- Galectins and Cancer Biology
- Plant Genetic and Mutation Studies
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Plant biochemistry and biosynthesis
- Diabetes Management and Research
- Protein Kinase Regulation and GTPase Signaling
- Allelopathy and phytotoxic interactions
- Integrated Energy Systems Optimization
- Diabetes Management and Education
- Retinal Diseases and Treatments
- Plant and animal studies
- Hybrid Renewable Energy Systems
- Artificial Intelligence in Healthcare
- Pancreatic function and diabetes
- Genetically Modified Organisms Research
Shanghai Jiao Tong University
2010-2025
Ruijin Hospital
2024-2025
Institute of Botany
2014-2024
Chinese Academy of Sciences
2014-2024
Beijing Botanical Garden
2014-2024
Xinjiang University
2024
China National Botanical Garden
2024
University of Chinese Academy of Sciences
2009-2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2023
Shenyang Pharmaceutical University
2021-2023
IMPORTANCE Noncommunicable chronic diseases have become the leading causes of mortality and disease burden worldwide.OBJECTIVE To investigate prevalence diabetes glycemic control in Chinese adult population.DESIGN, SETTING, AND PARTICIPANTS Using a complex, multistage, probability sampling design, we conducted cross-sectional survey nationally representative sample 98 658 adults 2010.MAIN OUTCOMES MEASURES Plasma glucose hemoglobin A 1c levels were measured after at least 10-hour overnight...
Phosphatase of regenerating liver 3 (PRL3) is overexpressed in a variety tumors, and high levels PRL3 expression are associated with tumorigenesis metastasis. Consistent an oncogenic role for PRL3, we show that ectopic promotes cell proliferation invasion. However, little known about the molecular basis function. Obtaining this knowledge vital understanding PRL3-mediated disease processes development novel anticancer therapies targeted to PRL3. Here report up-regulation activates Src kinase,...
Abstract For grain crops such as rice (Oryza sativa), size substantially affects yield. The histone acetyltransferase GRAIN WEIGHT 6a (GW6a) determines and yield in rice. However, the gene regulatory network underlying GW6a-mediated regulation of has remained elusive. In this study, we show that GW6a interacts with HOMOLOG OF DA1 ON RICE CHROMOSOME 3 (HDR3), a ubiquitin-interacting motif-containing ubiquitin receptor. Transgenic plants overexpressing HDR3 produced larger grains, whereas...
Seed vigor in crops is important terms of improving grain quality and germplasm conservation; however, little known about its regulatory mechanisms through the encoded proteome gene network. Comparative analyses transcriptome (RNA sequencing [RNA-seq]) broadly targeted metabolic profiling two subspecific rice cultivars with distinct seed during accelerated aging revealed various biological pathways processes as key influences explaining trait differences. RNA-seq coexpression network...
To compare the probability of achieving diabetes remission in individuals with different phenotypes insulin sensitivity, secretion, and beta cell function further detect effects diet, exercise, lifestyle education intervention on these indexes. Three-hundred one participants who had glycated haemoglobin (HbA1c) data at baseline after were included for this post hoc analysis. We used multi-way analysis variance to assess differences between non-remission groups or changes indexes function....
Protein tyrosine phosphatases (PTPs) are involved in the regulation of many aspects cellular activity including proliferation, differentiation, metabolism, migration, and survival. Given large number complexity PTPs cell signaling, new strategies needed for integrated analysis whole proteome. Unfortunately, activities tightly regulated by posttranslational mechanisms, limiting utility standard genomics proteomics methods functional characterization these enzymes. To facilitate global PTPs,...
Grain size is a key component of grain yield and quality in crops. Several core players auxin signaling have been revealed to modulate size; however, date, few genetically defined pathways reported, whether phosphorylation could boost degradation Aux/IAA proteins uncertain. Here, we show that TGW3 (also called OsGSK5) interacts with phosphorylates OsIAA10. Phosphorylation OsIAA10 facilitates its interaction OsTIR1 subsequent destabilization, but this modification hinders OsARF4. Our genetic...
The phosphatase of regenerating liver (PRL) phosphatases are implicated in a number tumorigenesis and metastasis processes. PRLs unique among protein-tyrosine that they have extremely low activity, high propensity for trimer formation, polybasic region precedes the C-terminal prenylation motif. To investigate functional significance these distinctive biochemical structural features, we established cell-based system which ectopic PRL1 expression increased cell proliferation migration, whereas...
Seed germination is a critical phase in the plant life cycle, but specific events associated with seed are still not fully understood. In this study, we used two‐dimensional gel electrophoresis followed by mass spectrometry to investigate changes proteome during imbibition of Oryza sativa seeds at optimal temperature or without abscisic acid ( ABA ) and high (germination thermoinhibition) further identify quantify key proteins required for germination. A total 121 protein spots showed...
Abstract The chromatin modifier GRAIN WEIGHT 6a (GW6a) enhances rice grain size and yield. However, little is known about its gene network determining size. Here, we report that MITOGEN-ACTIVED PROTEIN KINASE 6 (OsMAPK6) E3 ligase CHANG LI GENG 1 (CLG1) interact with target GW6a for phosphorylation ubiquitylation, respectively. Unexpectedly, however, in vitro vivo assays reveal both of the two post-translational modifications stabilize GW6a. Furthermore, uncover major sites (serine142...
The PRL (phosphatase of regenerating liver) phosphatases constitute a novel class small, prenylated that are implicated in promoting cell growth, differentiation, and tumor invasion, represent attractive targets for anticancer therapy. Here we describe the crystal structures native PRL-1 as well catalytically inactive mutant PRL-1/C104S complex with sulfate. exists trimer crystalline state, burying 1140 A2 accessible surface area at each dimer interface. Trimerization creates large,...
Seed vigor is a complex property that determines the seed's potential for rapid uniform emergence and subsequent growth. However, mechanism change in seed poorly understood. The seeds of poplar (Populus × Canadensis Moench), which are short-lived, were stored at 30°C 75±5% relative humidity different periods time (0–90 days) to obtain (from 95 0% germination). With decreasing vigor, temperature range germination became narrower; respiration rate decreased markedly, while electrolyte leakage...
Sample preparation is a critical process for proteomic studies. Many efficient and reproducible sample methods have been developed mass spectrometry-based analysis of human animal tissues or cells, but no attempt has made to evaluate these protocols plants. We here present an LC–MS/MS-based proteomics study barley leaf aimed at optimization achieve unbiased trypsin digestion proteins prior LC–MS/MS based sequencing quantification peptides. evaluated two spin filter-aided using either sodium...
We have studied the role(s) of maturation drying in acquisition germinability, seedling vigor and pathogen resistance by comparing proteome changes maize embryo endosperm during mature prematurely imposed drying. Prematurely dried seeds at 40 days after pollination (DAP) germinated almost as well (at 65 DAP), but their growth was slower they were seriously infected fungi. A total 80 114 proteins identified to change least two-fold (p < 0.05) abundance endosperm, respectively. Fewer (48 59...
Seed ageing is a process that results in delayed germination, decreased germination percentage, and finally total loss of seed viability. However, the mechanism poorly understood. In present study, Yliangyou 2 hybrid rice (Oryza sativa L.) seeds were artificially aged at 100% relative humidity 40°C, effect artificial on time course change protein profiles embryo endosperm was studied to understand molecular behind ageing. With an increasing duration ageing, percentage rate decreased. By...
Viscum coloratum (Kom.) Nakai is a well-known medicinal hemiparasite widely distributed in Asia. The synthesis and accumulation of its metabolites are affected by both environmental factors the host plants, while latter which usually overlooked. purpose this study was to comprehensively evaluate effects habitat on V. through multiple chemical biological approaches. metabolite profile harvested from three different plants two habitats were determined methods including high-performance liquid...
Inositol-5-phosphatases are important enzymes involved in the regulation of diverse cellular processes from synaptic vesicle recycling to insulin signaling. We describe a comparative study two representative inositol-5-phosphatases, Schizosaccharomyces pombe synaptojanin (SPsynaptojanin) and human SH2 domain-containing inositol-5-phosphatase SHIP2. show that addition Mg2+, transition metals such as Mn2+, Co2+, Ni2+ also effective activators SPsynaptojanin. In contrast, Ca2+ Cu2+ inhibitory....
Mitochondrial repair is of fundamental importance for seed germination. When mature orthodox seeds are imbibed and germinated, they lose their desiccation tolerance in parallel. To gain a better understanding this process, we studied the recovery mitochondrial structure function pea (Pisum sativum cv. Jizhuang) with different to desiccation. Mitochondria were isolated purified from embryo axes control imbibed-dehydrated after (re-)imbibition various times. Recovery occurred both axes, but at...
Germination and thermoinhibition in lettuce (Lactuca sativa 'Jianyexianfeng No. 1') seeds were investigated by a proteomic comparison among dry seeds, germinated at 15°C, 15°C after imbibition 25°C for 48 h, or KNO3 (all sampled individually germination), ungerminated 25°C, thermoinhibitory temperature. Before two-dimensional gel electrophoresis analysis, storage proteins (greater than 50% of total extractable protein) removed polyethylene glycol precipitation, which significantly improved...